A novel DNA tetrahedron–hairpin probe for in situ “off–on” fluorescence imaging of intracellular telomerase activity

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2474-2480 ◽  
Author(s):  
Qiu-Mei Feng ◽  
Meng-Jiao Zhu ◽  
Ting-Ting Zhang ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Fluorescence Imaging ◽  
Telomerase Activity ◽  
Recovery Efficiency ◽  
High Recovery ◽  
Hairpin Probe ◽  
Dna Tetrahedron

A DNA tetrahedron–hairpin probe with a high recovery efficiency is designed for in situ fluorescence imaging of intracellular telomerase activity.

scholarly journals Facile fabrication of ion-imprinted Fe3O4/carboxymethyl cellulose magnetic biosorbent: removal and recovery properties for trivalent La ions

RSC Advances ◽  
2021 ◽  
Vol 11 (41) ◽  
pp. 25258-25265
Author(s):  
Long Liu ◽  
Sheng Chang ◽  
Yan Wang ◽  
Hexiang Zhao ◽  
Shuteng Wang ◽  
...  
Keyword(s):  
Carboxymethyl Cellulose ◽  
Recovery Efficiency ◽  
High Recovery ◽  
Ion Imprinted ◽  
Facile Fabrication ◽  
Ion Imprinting ◽  
Removal And Recovery

An Fe3O4/carboxymethyl cellulose magnetic biosorbent was prepared by ion-imprinting technology, showing good adsorption and selectivity properties for La(iii) with a high recovery efficiency.

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

scholarly journals Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

2020 ◽  
Vol 8 (1) ◽  
pp. 53
Author(s):  
Thomas Oh ◽  
Jittiwat Sermsripong ◽  
Barry W. Hicks
Keyword(s):  
Fluorescence Imaging ◽  
Large Scale ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Florida Keys ◽  
Nir Fluorescence ◽  
Novel Method ◽  
Near Infrared Fluorescence Imaging

Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

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