Sustainability assessment of succinic acid production technologies from biomass using metabolic engineering

2016 ◽  
Vol 9 (9) ◽  
pp. 2794-2805 ◽  
Author(s):  
Merten Morales ◽  
Meriç Ataman ◽  
Sara Badr ◽  
Sven Linster ◽  
Ioannis Kourlimpinis ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Environmental Impact ◽  
Succinic Acid ◽  
Acid Production ◽  
Sustainability Assessment ◽  
State Of The Art ◽  
Process Modelling ◽  
Economic Feasibility ◽  
Production Technologies ◽  
Succinic Acid Production

Innovative pathways for bio-succinic acid production from biomass are investigated regarding their environmental impact, economic feasibility and process hazard by including state of the art metabolic engineering in process modelling.

scholarly journals Metabolic Engineering of Actinobacillus succinogenes Provides Insights into Succinic Acid Biosynthesis

2017 ◽  
Vol 83 (17) ◽  
Author(s):  
Michael T. Guarnieri ◽  
Yat-Chen Chou ◽  
Davinia Salvachúa ◽  
Ali Mohagheghi ◽  
Peter C. St. John ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Succinic Acid ◽  
Acid Production ◽  
Tca Cycle ◽  
Economic Feasibility ◽  
High Yield ◽  
Actinobacillus Succinogenes ◽  
Acid Biosynthesis ◽  
Content Type ◽  
Succinic Acid Production

ABSTRACT Actinobacillus succinogenes, a Gram-negative facultative anaerobe, exhibits the native capacity to convert pentose and hexose sugars to succinic acid (SA) with high yield as a tricarboxylic acid (TCA) cycle intermediate. In addition, A. succinogenes is capnophilic, incorporating CO2 into SA, making this organism an ideal candidate host for conversion of lignocellulosic sugars and CO2 to an emerging commodity bioproduct sourced from renewable feedstocks. In this work, we report the development of facile metabolic engineering capabilities in A. succinogenes, enabling examination of SA flux determinants via knockout of the primary competing pathways—namely, acetate and formate production—and overexpression of the key enzymes in the reductive branch of the TCA cycle leading to SA. Batch fermentation experiments with the wild-type and engineered strains using pentose-rich sugar streams demonstrate that the overexpression of the SA biosynthetic machinery (in particular, the enzyme malate dehydrogenase) enhances flux to SA. Additionally, removal of competitive carbon pathways leads to higher-purity SA but also triggers the generation of by-products not previously described from this organism (e.g., lactic acid). The resultant engineered strains also lend insight into energetic and redox balance and elucidate mechanisms governing organic acid biosynthesis in this important natural SA-producing microbe. IMPORTANCE Succinic acid production from lignocellulosic residues is a potential route for enhancing the economic feasibility of modern biorefineries. Here, we employ facile genetic tools to systematically manipulate competing acid production pathways and overexpress the succinic acid-producing machinery in Actinobacillus succinogenes. Furthermore, the resulting strains are evaluated via fermentation on relevant pentose-rich sugar streams representative of those from corn stover. Overall, this work demonstrates genetic modifications that can lead to succinic acid production improvements and identifies key flux determinants and new bottlenecks and energetic needs when removing by-product pathways in A. succinogenes metabolism.

Economic feasibility of a pilot-scale fermentative succinic acid production from bakery wastes

2014 ◽  
Vol 92 (3) ◽  
pp. 282-290 ◽  
Author(s):  
Koon Fung Lam ◽  
Cho Chark Joe Leung ◽  
Ho Man Lei ◽  
Carol Sze Ki Lin
Keyword(s):  
Succinic Acid ◽  
Acid Production ◽  
Pilot Scale ◽  
Economic Feasibility ◽  
Succinic Acid Production

scholarly journals Using genome-scale model to predict the metabolic engineering impact on Escherichia coli metabolism during succinic acid production optimization

2020 ◽  
Vol 25 (3) ◽  
pp. 1666-1676
Author(s):  
ZSOLT BODOR ◽  
◽  
ANDREA (IUHÁSZ) FAZAKAS ◽  
KATALIN BODOR ◽  
ERIKA KOVÁCS ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Succinic Acid ◽  
Acid Production ◽  
Production Optimization ◽  
Scale Model ◽  
Succinic Acid Production ◽  
Genome Scale ◽  
Genome Scale Model

scholarly journals Sustainability assessment of levulinic acid and succinic acid production from empty fruit bunch

2020 ◽  
Vol 778 ◽  
pp. 012140
Author(s):  
R H Hafyan ◽  
L Bhullar ◽  
Z A Putra ◽  
M R Bilad ◽  
M D H Wirzal ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Acid Production ◽  
Levulinic Acid ◽  
Sustainability Assessment ◽  
Empty Fruit Bunch ◽  
Succinic Acid Production

Bioprocess development using organic biowaste and sustainability assessment of succinic acid production with engineered Yarrowia lipolytica strain

2021 ◽  
pp. 108099
Author(s):  
Eleni Stylianou ◽  
Chrysanthi Pateraki ◽  
Dimitrios Ladakis ◽  
Christina Damala ◽  
Anestis Vlysidis ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Sustainability Assessment ◽  
Bioprocess Development ◽  
Lipolytica Strain ◽  
Succinic Acid Production
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