Dual signal amplification by an “on-command” pure DNA hydrogel encapsulating HRP for colorimetric detection of ochratoxin A

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114500-114504 ◽  
Author(s):  
Lu Zhou ◽  
Na Sun ◽  
Lijun Xu ◽  
Xing Chen ◽  
Hui Cheng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Ochratoxin A ◽  
Signal Amplification ◽  
Colorimetric Detection ◽  
Dual Signal Amplification ◽  
Dna Hydrogel ◽  
Aptamer Domain

A pure DNA hydrogel, consisting of two kinds of Y-scaffold nucleic acid subunits and the aptamer domain of ochratoxin A, undergoes a switchable gel-to-sol transition in the presence of ochratoxin A.

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification

2019 ◽  
Vol 186 (4) ◽  
Author(s):  
Weidan Chang ◽  
Weipeng Liu ◽  
Ying Liu ◽  
Fangfang Zhan ◽  
Huifang Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Plant Pathogens ◽  
Colorimetric Detection ◽  
Nucleic Acid Sequences

scholarly journals Multiplex microRNA imaging in living cells using DNA-capped-Au assembled hydrogels

2018 ◽  
Vol 9 (37) ◽  
pp. 7419-7425 ◽  
Author(s):  
Xiangdan Meng ◽  
Kai Zhang ◽  
Wenhao Dai ◽  
Yu Cao ◽  
Fan Yang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Living Cells ◽  
Hydrogel Network ◽  
Dual Signal Amplification ◽  
Dna Hydrogel

We designed a porous 3D Au–DNA hydrogel network for simultaneously imaging multiplex miRNAs in living cells through dual-signal amplification.

Sensitive and selective colorimetric detection of Hg2+ by a Hg2+ induced dual signal amplification strategy based on cascade-type catalytic reactions

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2362-2366 ◽  
Author(s):  
Suping Li ◽  
Jianping Lai ◽  
Liming Qi ◽  
Muhammad Saqib ◽  
Saadat Majeed ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Catalytic Reactions ◽  
Selective Detection ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Cascade Type

A simple and fast colorimetric method is developed for the sensitive and selective detection of Hg2+ based on a dual signal amplification strategy.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

A label-free aptasensor for ochratoxin a detection with signal amplification strategies on ultrathin micron-sized 2D MOF sheets

2021 ◽  
Vol 334 ◽  
pp. 129682
Author(s):  
Xiujuan Qiao ◽  
Xin Ma ◽  
Xiaoyu Ma ◽  
Tianli Yue ◽  
Qinglin Sheng
Keyword(s):  
Ochratoxin A ◽  
Signal Amplification ◽  
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