Cobalt disulfide nanowires as an effective fluorescent sensing platform for DNA detection

2016 ◽  
Vol 4 (17) ◽  
pp. 2860-2863 ◽  
Author(s):  
Zhicai Xing ◽  
Lei Wang ◽  
Xiurong Yang

Cobalt disulfide nanowires are synthesized in solution using a facile two-step hydrothermal method for the first time and applied as an effective sensing platform for nucleic acid detection.

2017 ◽  
Vol 1 (6) ◽  
pp. 1130-1136 ◽  
Author(s):  
Ying Teng Yew ◽  
Zdeněk Sofer ◽  
Carmen C. Mayorga-Martinez ◽  
Martin Pumera

Black phosphorus nanoparticles were successfully employed as nanofluorophores in the development of a fluorescent sensing platform for DNA detection.


2015 ◽  
Vol 3 (36) ◽  
pp. 7173-7176 ◽  
Author(s):  
Zhicai Xing ◽  
Lei Wang ◽  
Xuping Sun ◽  
Yuquan He ◽  
Abdullah M. Asiri

Nanoporous Mo2C nanowires have been successfully demonstrated as an effective sensing platform for nucleic acid detection for the first time.


2011 ◽  
Vol 47 (21) ◽  
pp. 6099 ◽  
Author(s):  
Shuyun Zhu ◽  
Zhongyuan Liu ◽  
Wei Zhang ◽  
Shuang Han ◽  
Lianzhe Hu ◽  
...  

2010 ◽  
Vol 39 (6) ◽  
pp. e37-e37 ◽  
Author(s):  
Lei Wang ◽  
Yingwei Zhang ◽  
Jingqi Tian ◽  
Hailong Li ◽  
Xuping Sun

2011 ◽  
Vol 175 (3-4) ◽  
pp. 355-359 ◽  
Author(s):  
Wenbo Lu ◽  
Xiaoyun Qin ◽  
Yonglan Luo ◽  
Guohui Chang ◽  
Xuping Sun

2011 ◽  
Vol 47 (3) ◽  
pp. 961-963 ◽  
Author(s):  
Hailong Li ◽  
Yingwei Zhang ◽  
Lei Wang ◽  
Jingqi Tian ◽  
Xuping Sun

2011 ◽  
Vol 32 (12) ◽  
pp. 899-904 ◽  
Author(s):  
Hailong Li ◽  
Lei Wang ◽  
Junfeng Zhai ◽  
Yingwei Zhang ◽  
Jingqi Tian ◽  
...  

Author(s):  
Xinhui Xu ◽  
Tao Luo ◽  
Jinliang Gao ◽  
Na Lin ◽  
Weiwei Li ◽  
...  

AbstractNucleic acid detection techniques are always critical to diagnosis, especially in the background of the present COVID-19 pandemic. The simple and rapid detection techniques with high sensitivity and specificity are always urgently needed. However, the current nucleic acid detection techniques are still limited the traditional amplification and hybridization. To overcome the limitation, we here develop a CRISPR/Cas9-assisted DNA detection (CADD). In this detection, DNA sample is incubated with a pair of capture sgRNAs (sgRNAa and sgRNAb) specific to a target DNA, dCas9, a signal readout-related probe, and an oligo-coated solid support beads or microplate at room temperature for 15 min. During this incubation, the dCas9-sgRNA-DNA complex is formed and captured on solid support by the capture sequence of sgRNAa and the signal readout-related probe is captured by the capture sequence of sgRNAb. Finally the detection result is reported by a fluorescent or colorimetric signal readout. This detection was verified by detecting DNA of bacteria, cancer cell and virus. Especially, by designing a set of sgRNAs specific to 15 high-risk human papillomaviruses (HPVs), the HPV infection in 64 clinical cervical samples were successfully detected by the method. All detections can be finished in 30 minutes at room temperature. This detection holds promise for rapid on-the-spot detection or point-of-care testing (POCT).


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