In vitro and in vivo evaluation of the effect of nano-sized collagen molecules and nicotinamide on mesenchymal stem cell differentiation

2016 ◽  
Vol 4 (22) ◽  
pp. 3892-3902 ◽  
Author(s):  
Chin-Tsu Ma ◽  
Yi-Jhen Wu ◽  
Han Hsiang Huang ◽  
Pei-Leun Kang ◽  
Kuan Yin Hsiao ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Stem Cell Differentiation ◽  
In Vivo Evaluation ◽  
Cell Replacement ◽  
Tissue Repair And Regeneration ◽  
Mesenchymal Stem Cell Differentiation ◽  
Collagen Molecules ◽  
Stromal Stem Cells

Advances and improvements in mesenchymal stromal/stem cells (MSCs) and cell replacement therapies have been promising approaches to treat diabetes mellitus (DM) since their potent capacities for differentiation into various functional cells match the demands of tissue repair and regeneration.

Current Status of Used Protocols for Mesenchymal Stem Cell Differentiation: A Focus on Insulin Producing, Osteoblast-Like and Neural Cells

2019 ◽  
Vol 14 (7) ◽  
pp. 570-578 ◽  
Author(s):  
Hadi Rajabi ◽  
Vahid Hosseini ◽  
Sevda Rahimzadeh ◽  
Nayer Seyfizadeh ◽  
Somayeh Aslani ◽  
...  
Keyword(s):  
High Capacity ◽  
Cell Lineage ◽  
Stem Cell Differentiation ◽  
Current Status ◽  
Neural Cells ◽  
Differentiated Cells ◽  
Mesenchymal Stem Cell Differentiation ◽  
Main Requirement

: Mesenchymal stem cells (MSCs) have attracted a great deal of interest in the field of regenerative medicine because of their ability to differentiate into mesodermal derivatives and even other germ layers. The main requirement for better differentiation of MSCs into desired cell lineage is relied on pure population of these cells. During the past years, significant progresses have been developed for the identification of MSCs by introducing new markers or different combination of markers. Currently, direct in vitro differentiation protocols using standard media supplemented with specific growth factors generating osteoblast, insulin producing and neuron cells from MSCs show some key characteristic in in vivo counterparts. However, these efforts should be continued to achieve high amount of fully differentiated cells which have high capacity to be used in cell based therapies and drug screening. This review focuses on common culture based differentiation strategies used for osteoblast, insulin producing cells and neural cells generation from MSCs highlighting important findings and trends in this exciting area.

A Porous Hydroxyapatite/Gelatin Nanocomposite Scaffold for Bone Tissue Repair: In Vitro and In Vivo Evaluation

2012 ◽  
Vol 23 (18) ◽  
pp. 2353-2368 ◽  
Author(s):  
Mahmoud Azami ◽  
Shima Tavakol ◽  
Ali Samadikuchaksaraei ◽  
Mehran Solati Hashjin ◽  
Nafiseh Baheiraei ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Repair ◽  
In Vivo Evaluation ◽  
Porous Hydroxyapatite ◽  
Nanocomposite Scaffold

scholarly journals The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Jianqun Ma ◽  
Kyle Holden ◽  
Jinhong Zhu ◽  
Haiying Pan ◽  
Yong Li
Keyword(s):  
Soft Tissue ◽  
Muscle Tissue ◽  
Tissue Repair ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Tissue Repair And Regeneration ◽  
Tissue Constructs ◽  
Defect Sites

Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graftin vitro. The 3D graft createdin vitrowas then utilized to investigate muscle tissue repairin vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graftin vitrothat can be employed for defect muscle tissue repairin vivo.

scholarly journals Modern concepts of the osteogenic potential of mesenchymal stem cells and the creation of bioengineering structures for bone tissue repair

2020 ◽  
Vol 17 (4) ◽  
pp. 500-508
Author(s):  
H. A. Zhernasechanka
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Tissue Repair ◽  
Stem Cell Differentiation ◽  
Osteogenic Potential ◽  
The Novel ◽  
Mesenchymal Stem Cell Differentiation ◽  
Engineered Tissue ◽  
The Creation

The following review summarizes the latest studies on in vitro osteogenic mesenchymal stem cell differentiation and selection of scaffolds that can maintain the viability and functional activity of these cells for bone tissue repair. In the last time, there have been investigated a lot of issues such as the stimulation and development osteogenic differentiation of MSCs, the growth factors – inducers of osteogenesis in MSCs, the creation of 3D constructions of cells in different scaffolds. A deeper understanding of the osteogenic differentiation mechanisms can result in the novel therapeutic opportunities of bone disease treatment. Special attention is given to materials for scaffold designs and template–cell interactions, which is of great importance for the structuring and functioning of an engineered tissue.

scholarly journals Effects of Silicon Compounds on Biomineralization, Osteogenesis, and Hard Tissue Formation

Pharmaceutics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 117 ◽  
Author(s):  
Werner Götz ◽  
Edda Tobiasch ◽  
Steffen Witzleben ◽  
Margit Schulze
Keyword(s):  
Stem Cell ◽  
Stem Cell Differentiation ◽  
In Vivo Studies ◽  
Special Focus ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Silicon Compounds ◽  
Mesenchymal Stem Cell Differentiation

Bioinspired stem cell-based hard tissue engineering includes numerous aspects: The synthesis and fabrication of appropriate scaffold materials, their analytical characterization, and guided osteogenesis using the sustained release of osteoinducing and/or osteoconducting drugs for mesenchymal stem cell differentiation, growth, and proliferation. Here, the effect of silicon- and silicate-containing materials on osteogenesis at the molecular level has been a particular focus within the last decade. This review summarizes recently published scientific results, including material developments and analysis, with a special focus on silicon hybrid bone composites. First, the sources, bioavailability, and functions of silicon on various tissues are discussed. The second focus is on the effects of calcium-silicate biomineralization and corresponding analytical methods in investigating osteogenesis and bone formation. Finally, recent developments in the manufacturing of Si-containing scaffolds are discussed, including in vitro and in vivo studies, as well as recently filed patents that focus on the influence of silicon on hard tissue formation.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

Fenofibrate Solid Dispersion for Improving Oral Bioavailability: Preparation, and Characterization and in vivo Evaluation

2020 ◽  
Vol 13 (5) ◽  
pp. 5102-5109
Author(s):  
Venu Madhav K ◽  
Somnath De ◽  
Chandra Shekar Bonagiri ◽  
Sridhar Babu Gummadi
Keyword(s):  
Oral Bioavailability ◽  
Oral Delivery ◽  
Solid Dispersions ◽  
In Vitro Release ◽  
Aqueous Solubility ◽  
Water Soluble ◽  
Scanning Calorimetry ◽  
In Vivo Evaluation

Fenofibrate (FN) is used in the treatment of hypercholesterolemia. It shows poor dissolution and poor oral bioavailability after oral administration due to high liphophilicity and low aqueous solubility. Hence, solid dispersions (SDs) of FN (FN-SDs) were develop that might enhance the dissolution and subsequently oral bioavailability. FN-SDs were prepared by solvent casting method using different carriers (PEG 4000, PEG 6000, β cyclodextrin and HP β cyclodextrin) in different proportions (0.25%, 0.5%, 0.75% and 1% w/v). FN-SDs were evaluated solubility, assay and in vitro release studies for the optimization of SD formulation. Differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) analysis was performed for crystalline and morphology analysis, respectively. Further, optimized FN-SD formulation evaluated for pharmacokinetic performance in Wistar rats, in vivo in comparison with FN suspension.  From the results, FN-SD3 and FN-SD6 have showed 102.9 ±1.3% and 105.5±3.1% drug release, respectively in 2 h. DSC and PXRD studies revealed that conversion of crystalline to amorphous nature of FN from FT-SD formulation. SEM studies revealed the change in the orientation of FN when incorporated in SDs. The oral bioavailability FN-SD3 and FN-SD6 formulations exhibited 2.5-folds and 3.1-folds improvement when compared to FN suspension as control. Overall, SD of FN could be considered as an alternative dosage form for the enhancement of oral delivery of poorly water-soluble FN.

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