scholarly journals Improved molecular level identification of organic compounds using comprehensive two-dimensional chromatography, dual ionization energies and high resolution mass spectrometry

The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2395-2403 ◽  
Author(s):  
David R. Worton ◽  
Monika Decker ◽  
Gabriel Isaacman-VanWertz ◽  
Arthur W. H. Chan ◽  
Kevin R. Wilson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Organic Compounds ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
Two Dimensional ◽  
Analytical Methodology ◽  
Complex Samples ◽  
Level Identification ◽  
Resolution Mass

A new analytical methodology has been developed to improve molecular level identification of organic compounds in complex samples.

scholarly journals A comparison of one-dimensional and microscale two-dimensional liquid chromatographic approaches coupled to high resolution mass spectrometry for the analysis of complex samples

2015 ◽  
Vol 7 (18) ◽  
pp. 7697-7706 ◽  
Author(s):  
Juri Leonhardt ◽  
Thorsten Teutenberg ◽  
Jochen Tuerk ◽  
Michael P. Schlüsener ◽  
Thomas A. Ternes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Two Dimensional ◽  
Complex Samples ◽  
One Dimensional ◽  
Open Questions ◽  
Liquid Chromatographic ◽  
Resolution Mass

The interest in two-dimensional liquid chromatography separations is growing every year together with the number of open questions on the benefits.

Molecular-level heavy petroleum hydrotreating modeling and comparison with high-resolution mass spectrometry

Fuel ◽  
2021 ◽  
Vol 297 ◽  
pp. 120792
Author(s):  
Dong Guan ◽  
Zhengyu Chen ◽  
Xiu Chen ◽  
Ying Zhang ◽  
Qiuyan Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
Heavy Petroleum ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals Ircinal E, a New Manzamine Derivative from the Indonesian Marine Sponge Acanthostrongylophora ingens

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Mousa AlTarabeen ◽  
Georgios Daletos ◽  
Weaam Ebrahim ◽  
Werner E. G. Müller ◽  
Rudolf Hartmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
High Resolution ◽  
Cell Line ◽  
Marine Sponge ◽  
High Resolution Mass Spectrometry ◽  
Two Dimensional ◽  
Meoh Extract ◽  
L5178y Cell ◽  
Resolution Mass

Chemical investigation of the MeOH extract of the sponge Acanthostrongylophora ingens afforded the new manzamine derivative ircinal E (1), in addition to six known metabolites (2–7). The structure of the new compound was unequivocally elucidated using one- and two-dimensional NMR spectroscopy, as well as high-resolution mass spectrometry. Compounds 1–6 exhibited strong to moderate cytotoxicity against the murine lymphoma L5178Y cell line with IC50 values ranging from 2.8 to 21.7 μM.

Probing the Mechanism of Action of Potential Anticancer Agents at a Molecular Level Using Electrospray Ionisation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

2009 ◽  
Vol 15 (5) ◽  
pp. 661-672 ◽  
Author(s):  
Lutz F. Tietze ◽  
Birgit Krewer ◽  
Holm Frauendorf
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Anticancer Agents ◽  
Mode Of Action ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
New Drugs ◽  
Duplex Dna ◽  
High Resolution Mass ◽  
Resolution Mass

Treating cancer without harming healthy tissue is an important goal in modern medicine. Our research group has developed a series of novel, relatively non-toxic glycosidic prodrugs that are activated to give the corresponding highly cytotoxic drugs selectively in the tumour tissue. Our first investigations have shown a high duplex DNA alkylation efficiency of the drugs, whereas the prodrugs showed almost no tendency for alkylation of duplex DNA. Herein we report on novel investigations of the mode of action of the anti-cancer drugs on a molecular level. Using high-resolution mass spectrometry, we determined the reactivity of these drugs as well as of other drugs of similar structure against different nucleophiles such as RNA and the tripeptide glutathione. In addition, the new drugs were also tested for their interaction with duplex DNA. All compounds show a high reactivity against duplex DNA, whereas the alkylation efficiency regarding RNA and glutathione is only poor. Furthermore, the alkylation of duplex DNA correlates qualitatively but not quantitatively with the cytotoxicity of the drugs. Consequently, other factors besides the alkylation efficiency such as the stability of the drugs seem to influence their biological activity. Altogether the results show that high-resolution mass spectrometry constitutes a powerful method for studying the mode of action of drugs on a molecular level.

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