Simultaneous detection of superoxide anion radicals and determination of the superoxide scavenging activity of antioxidants using a N,N-dimethyl-p-phenylene diamine/Nafion colorimetric sensor

To address the problem of limited application of natural SOD, the development of SOD mimic enzymes is of great importance for bioantioxidation. Herein, we report on a new type of biopolymer antioxidant with excellent scavenging activity for O2•-, keratin-glycine metal complexes (FK-GlyM, M = Zn, Cu, Mn, Ni). They are prepared by feather keratin firstly combined with glycine and then metal ions. Using FT-IR, TG, CD, and SEM, the performance of the obtained complexes (FK-GlyM) for scavenging O2•- is analysed and investigated. Importantly, the scavenging activity of FK-GlyCu is excellent in all FK-GlyM, and FK-GlyCu60 has the most excellent anti-O2•- activity in all FK-GlyCux, of which EC50 and degree of simulation were, respectively, up to 4.5×10-3±0.0012 μmol/L and 911.1% compared with nature Cu, Zn-SOD. Finally, its mechanism was also discussed. In summary, this method about the simulation strategies will provide a novel idea for exploiting new-type biocompatible and highly reactive antioxidants.

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Scavenging activity of aqueous nanoparticle suspension of fullerene amino acids against superoxide anion radicals

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.07.969 ◽

2008 ◽

Vol 136 ◽

pp. S419 ◽

Cited By ~ 1

Author(s):

Jing Zhang ◽

Xinlin Yang

Keyword(s):

Amino Acids ◽

Superoxide Anion ◽

Scavenging Activity ◽

Nanoparticle Suspension ◽

Superoxide Anion Radicals ◽

Anion Radicals

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Inclusion Complexes of Lycopene and β-Cyclodextrin: Preparation, Characterization, Stability and Antioxidant Activity

Antioxidants ◽

10.3390/antiox8080314 ◽

2019 ◽

Vol 8 (8) ◽

pp. 314 ◽

Cited By ~ 8

Author(s):

Haixiang Wang ◽

Shaofeng Wang ◽

Hua Zhu ◽

Suilou Wang ◽

Jiudong Xing

Keyword(s):

Antioxidant Activity ◽

Inclusion Complexes ◽

Superoxide Anion ◽

Antioxidant Properties ◽

Phase Solubility ◽

Scavenging Activity ◽

Positive Effects ◽

Phase Solubility Study ◽

Superoxide Anion Radicals ◽

Anion Radicals

In this study, the inclusion complexes of lycopene with β-cyclodextrin (β-CD) were prepared by the precipitation method. Then the inclusion complexes were characterized by the scanning electron microscopy (SEM), ultraviolet-visible spectroscopy (UV), microscopic observation, liquid chromatography, differential scanning calorimetry (DSC) and phase-solubility study. Moreover, the stability and antioxidant activity were tested. The results showed that lycopene was embedded into the cavity of β-CD with a 1:1 stoichiometry. Moreover, the thermal and irradiant stabilities of lycopene were all significantly increased by the formation of lycopene/β-CD inclusion complexes. Antioxidant properties of lycopene and its inclusion complexes were evaluated on the basis of measuring the scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals. The results showed that the scavenging activity of DPPH radicals was obviously increased by the formation of the inclusion complex with β-cyclodextrin at concentrations of 5–30 μg/mL, however, some significant positive effects on the scavenging activity of hydroxyl and superoxide anion radicals were not observed and the reasons are worth further study.

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