A silencing-mediated enhancement of osteogenic differentiation by supramolecular ternary siRNA polyplexes comprising biocleavable cationic polyrotaxanes and anionic fusogenic peptides

2018 ◽  
Vol 6 (2) ◽  
pp. 440-450 ◽  
Author(s):  
Takasuke Inada ◽  
Atsushi Tamura ◽  
Masahiko Terauchi ◽  
Satoshi Yamaguchi ◽  
Nobuhiko Yui
Keyword(s):  
Gene Silencing ◽  
Osteogenic Differentiation ◽  
Differentiation Efficiency ◽  
Fusogenic Peptides

The gene silencing of noggin by siRNA polyplexes composed of noggin-targeted siRNA, biocleavable cationic polyrotaxanes (DMAE-SS-PRX), and fusogenic GALA peptides was demonstrated to improve osteogenic differentiation efficiency.

scholarly journals Polyelectrolyte Complexes between Polycarboxylates and BMP-2 for Enhancing Osteogenic Differentiation: Effect of Chemical Structure of Polycarboxylates

Polymers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1327
Author(s):  
Masahiko Terauchi ◽  
Atsushi Tamura ◽  
Asato Tonegawa ◽  
Satoshi Yamaguchi ◽  
Tetsuya Yoda ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Chemical Structure ◽  
Early Stage ◽  
Anticoagulant Activity ◽  
Bone Morphogenetic Protein 2 ◽  
Clinical Use ◽  
Regenerative Therapy ◽  
Polyelectrolyte Complexes ◽  
Morphogenetic Protein ◽  
Differentiation Efficiency

Bone morphogenetic protein 2 (BMP-2) has received considerable attention because of its osteoinductivity, but its use is limited owing to its instability and adverse effects. To reduce the dose of BMP-2, complexation with heparin is a promising approach, because heparin enhances the osteoinductivity of BMP-2. However, the clinical use of heparin is restricted because of its anticoagulant activity. Herein, to explore alternative polymers that show heparin-like activity, four polycarboxylates, poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), poly(aspartic acid) (PAsp), and poly(glutamic acid) (PGlu), were selected and their capability to modulate the osteoinductivity of BMP-2 was evaluated. Dynamic light scattering indicated that these polycarboxylates formed polyelectrolyte complexes with BMP-2. The osteogenic differentiation efficiency of MC3T3-E1 cells treated with the polycarboxylate/BMP-2 complexes was investigated in comparison to that of the heparin/BMP-2 complex. As a result, PGlu/BMP-2 complex showed the highest activity of alkaline phosphatase, which is an early-stage marker of osteogenic differentiation, and rapid mineralization. Based on these observations, PGlu could serve as an alternative to heparin in the regenerative therapy of bone using BMP-2.

Gene Silencing of Chordin Improves BMP-2 Effects on Osteogenic Differentiation of Human Adipose Tissue-Derived Stromal Cells

2014 ◽  
Vol 20 (1-2) ◽  
pp. 335-345 ◽  
Author(s):  
Hellen Schneider ◽  
Bita Sedaghati ◽  
Andreas Naumann ◽  
Michael C. Hacker ◽  
Michaela Schulz-Siegmund
Keyword(s):  
Adipose Tissue ◽  
Gene Silencing ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Human Adipose Tissue

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure
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