Single-molecule imaging of telomerase activity via linear plasmon rulers

2017 ◽  
Vol 53 (34) ◽  
pp. 4710-4713 ◽  
Author(s):  
Guang-Sheng Qian ◽  
Ting-Ting Zhang ◽  
Wei Zhao ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Telomerase Activity ◽  
Single Molecule Imaging ◽  
Real Time Monitoring ◽  
First Time ◽  
Plasmon Rulers

A strategy for real-time monitoring of the extension of the telomerase primer based on plasmon rulers was demonstrated for the first time.

A multicolor DNA tetrahedron nanoprobe for analyzing human telomerase in living cells

2021 ◽  
Author(s):  
Ruiyuan Zhang ◽  
Ruixue Zhang ◽  
Wei Jiang ◽  
Xiaowen Xu
Keyword(s):  
Real Time ◽  
Length Distribution ◽  
Telomerase Activity ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Dna Tetrahedron ◽  
Human Telomerase

A sequentially lighting-up multicolor DNA tetrahedron nanoprobe is constructed for imaging telomerase activity, real-time monitoring telomerase action and determining product length distribution in living cells.

scholarly journals Complete Genome Sequence of Mycobacterium avium , Isolated from Commercial Domestic Pekin Ducks ( Anas platyrhynchos domestica ), Determined Using PacBio Single-Molecule Real-Time Technology

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Xiao-Heng Song ◽  
Hong-Xi Chen ◽  
Wang-Shu Zhou ◽  
Jiang-Bo Wang ◽  
Ma-Feng Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Genome Sequence ◽  
Mycobacterium Avium ◽  
Single Molecule ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virulent Strain ◽  
Domestic Ducks ◽  
Pekin Ducks ◽  
First Time

Mycobacterium avium is an important pathogenic bacterium in birds and has never, to our knowledge, reported to be isolated from domestic ducks. We present here the complete genome sequence of a virulent strain of Mycobacterium avium , isolated from domestic Pekin ducks for the first time, which was determined by PacBio single-molecule real-time technology.

scholarly journals Single-Molecule Imaging of RNA Polymerase-DNA Interactions in Real Time

1999 ◽  
Vol 76 (2) ◽  
pp. 709-715 ◽  
Author(s):  
Yoshie Harada ◽  
Takashi Funatsu ◽  
Katsuhiko Murakami ◽  
Yoshikazu Nonoyama ◽  
Akira Ishihama ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Real Time ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Dna Interactions

scholarly journals Single molecule imaging reveals a slice of life

2020 ◽  
Vol 153 (1) ◽  
Author(s):  
Ben Short
Keyword(s):  
Membrane Proteins ◽  
Real Time ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Tissue Slices ◽  
The Real ◽  
Live Tissue

JGP study describes method to trace the real-time movements of individual membrane proteins in live tissue slices.

scholarly journals Erratum: Real-time single-molecule imaging of quantum interference

2012 ◽  
Vol 7 (8) ◽  
pp. 544-544
Author(s):  
Thomas Juffmann ◽  
Adriana Milic ◽  
Michael Müllneritsch ◽  
Peter Asenbaum ◽  
Alexander Tsukernik ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Quantum Interference ◽  
Single Molecule Imaging

scholarly journals Understanding the diversity of DNA methylation in Mycobacterium tuberculosis

2020 ◽  
Author(s):  
Victor Ndhlovu ◽  
Anmol Kiran ◽  
Derek Sloan ◽  
Wilson Mandala ◽  
Marriot Nliwasa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Single Molecule ◽  
Complete Loss ◽  
Phenotypic Heterogeneity ◽  
Epigenetic Variation ◽  
Smrt Sequencing ◽  
Underlying Mechanisms ◽  
First Time

AbstractAlthough Mycobacterium tuberculosis (Mtb) strains exhibit genomic homology of >99%, there is considerable variation in the phenotype. The underlying mechanisms of phenotypic heterogeneity in Mtb are not well understood but epigenetic variation is thought to contribute. At present the methylome of Mtb has not been completely characterized. We completed methylomes of 18 Mycobacterium tuberculosis (Mtb) clinical isolates from Malawi representing the largest number of Mtb genomes to be completed in a single study using Single Molecule Real Time (SMRT) sequencing to date. We replicate and confirm four methylation disrupting mutations in lineages of Mtb. For the first time we report complete loss of methylation courtesy of C758T (S253L) mutation in the MamB gene of Indo-oceanic lineage of Mtb. We also conducted a genomic and methylome comparison of the Malawian samples against a global sample. We confirm that methylation in Mtb is lineage specific although some unresolved issues still remain.

scholarly journals Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits

2017 ◽  
Vol 53 (23) ◽  
pp. 3307-3310 ◽  
Author(s):  
Seungyong You ◽  
James Froberg ◽  
Junru Yu ◽  
Manas Haldar ◽  
Abbas Sedigh ◽  
...  
Keyword(s):  
Real Time ◽  
Histone Deacetylase ◽  
Single Molecule ◽  
Catalytic Reaction ◽  
Conformational Transitions ◽  
Real Time Monitoring ◽  
Conformational Interconversion ◽  
Histone Deacetylase 8

A novel, electronic single-molecule approach captures the dynamic conformational interconversion of HDAC8 and various ligands during the catalytic reaction.

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