In situ examination of a charged amino acid-induced structural change in lipid bilayers by sum frequency generation vibrational spectroscopy

2018 ◽  
Vol 20 (8) ◽  
pp. 5657-5665 ◽  
Author(s):  
Jiahui Zhang ◽  
Weilai Yang ◽  
Junjun Tan ◽  
Shuji Ye

Simultaneously capturing the structure perturbations of different lipid bilayer moiety allows us to discern the penetration depth of amino acid in cell membrane.

2015 ◽  
Vol 51 (1) ◽  
pp. 195-197 ◽  
Author(s):  
Khoi Tan Nguyen

Electronically enhanced chiral SFG spectroscopy was employed to study the lipid bound cyt c in situ. It was directly observed that upon interacting with anionic phospholipids, the amino acid residues around the heme adopted the β-sheet conformation. In addition, the orientation of this newly formed β-sheet structure was found to be sensitive to the bulk pH.


2009 ◽  
Vol 96 (2) ◽  
pp. 345-356 ◽  
Author(s):  
Orsolya Hakkel ◽  
Zoltán Pászti ◽  
Tamás Keszthelyi ◽  
Krisztina Frey ◽  
László Guczi

2019 ◽  
Vol 55 (4) ◽  
pp. 541-544 ◽  
Author(s):  
Shuji Ye ◽  
Junjun Tan ◽  
Kangzhen Tian ◽  
Chuanzhao Li ◽  
Jiahui Zhang ◽  
...  

Coherent degenerate infrared-infrared-visible sum frequency generation vibrational spectroscopy provides a powerful label-free probe for identifying the vibrational modes that are coupled through the electronic states in situ and in real time.


2016 ◽  
Vol 18 (3) ◽  
pp. 1411-1421 ◽  
Author(s):  
Heng-Liang Wu ◽  
Yujin Tong ◽  
Qiling Peng ◽  
Na Li ◽  
Shen Ye

The phase transition behaviors of a supported bilayer of dipalmitoylphosphatidyl-choline (DPPC) have been systematically evaluated by in situ sum frequency generation (SFG) vibrational spectroscopy and atomic force microscopy (AFM).


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