scholarly journals On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products

Lab on a Chip ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 162-170 ◽  
Author(s):  
Kadi L. Saar ◽  
Yingbo Zhang ◽  
Thomas Müller ◽  
Challa P. Kumar ◽  
Sean Devenish ◽  
...  
Keyword(s):  
Electric Fields ◽  
Single Layer ◽  
Microfluidic Devices ◽  
Protein Analysis ◽  
Label Free ◽  
Free Protein ◽  
On Chip ◽  
Electrolysis Products

Single-layer lithography microfluidic devices for applying high and stable electric fields on chip.

scholarly journals Single cell manipulation, analytics, and label-free protein detection in microfluidic devices for systems nanobiology

2005 ◽  
Vol 26 (19) ◽  
pp. 3689-3696 ◽  
Author(s):  
Wibke Hellmich ◽  
Christoph Pelargus ◽  
Kai Leffhalm ◽  
Alexandra Ros ◽  
Dario Anselmetti
Keyword(s):  
Single Cell ◽  
Protein Detection ◽  
Microfluidic Devices ◽  
Cell Manipulation ◽  
Label Free ◽  
Free Protein ◽  
Single Cell Manipulation

Label-Free Protein Analysis Using Liquid Chromatography with Gravimetric Detection

2021 ◽  
Vol 93 (5) ◽  
pp. 2848-2853
Author(s):  
Tadas Kartanas ◽  
Aviad Levin ◽  
Zenon Toprakcioglu ◽  
Tom Scheidt ◽  
Tuuli A. Hakala ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Protein Analysis ◽  
Label Free ◽  
Free Protein

On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity

2012 ◽  
Vol 174 ◽  
pp. 602-608 ◽  
Author(s):  
S.V. Pham ◽  
M. Dijkstra ◽  
A.J.F. Hollink ◽  
L.J. Kauppinen ◽  
R.M. de Ridder ◽  
...  
Keyword(s):  
Label Free ◽  
Protein Sensing ◽  
Free Protein ◽  
On Chip ◽  
Direct Label

Review of Microfluidic Devices for On-Chip Chemical Sensing

2016 ◽  
Vol 136 (6) ◽  
pp. 244-249
Author(s):  
Takahiro Watanabe ◽  
Fumihiro Sassa ◽  
Yoshitaka Yoshizumi ◽  
Hiroaki Suzuki
Keyword(s):  
Chemical Sensing ◽  
Microfluidic Devices ◽  
On Chip

scholarly journals Electrokinetic Formation of “Microbridges” for Protein Biomarkers as Sensors

2007 ◽  
Vol 12 (5) ◽  
pp. 311-317 ◽  
Author(s):  
Vindhya Kunduru ◽  
Shalini Prasad
Keyword(s):  
Electric Fields ◽  
Microelectrode Array ◽  
Protein Detection ◽  
High Specificity ◽  
Detection Methods ◽  
Label Free ◽  
Protein Biomarkers ◽  
Detection Scheme ◽  
Polystyrene Beads ◽  
Conducting Path

We demonstrate a technique to detect protein biomarkers contained in vulnerable coronary plaque using a platform-based microelectrode array (MEA). The detection scheme is based on the property of high specificity binding between antibody and antigen similar to most immunoassay techniques. Rapid clinical diagnosis can be achieved by detecting the amount of protein in blood by analyzing the protein's electrical signature. Polystyrene beads which act as transportation agents for the immobile proteins (antigen) are electrically aligned by application of homogenous electric fields. The principle of electrophoresis is used to produce calculated electrokinetic movement among the anti-C-reactive protein (CRP), or in other words antibody funtionalized polystyrene beads. The electrophoretic movement of antibody-functionalized polystyrene beads results in the formation of “Microbridges” between the two electrodes of interest which aid in the amplification of the antigen—antibody binding event. Sensitive electrical equipment is used for capturing the amplified signal from the “Microbridge” which essentially behaves as a conducting path between the two electrodes. The technique circumvents the disadvantages of conventional protein detection methods by being rapid, noninvasive, label-free, repeatable, and inexpensive. The same principle of detection can be applied for any receptor—ligand-based system because the technique is based only on the volume of the analyte of interest. Detection of the inflammatory coronary disease biomarker CRP is achieved at concentration levels spanning over the lower microgram/milliliter to higher order nanogram/milliliter ranges.

scholarly journals Comparative membrane proteomics: a technical advancement in the search of renal cell carcinoma biomarkers

2015 ◽  
Vol 11 (6) ◽  
pp. 1708-1716 ◽  
Author(s):  
Francesca Raimondo ◽  
Samuele Corbetta ◽  
Andrea Savoia ◽  
Clizia Chinello ◽  
Marta Cazzaniga ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Membrane Protein ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Protein Analysis ◽  
Comparative Proteomics ◽  
Label Free ◽  
Membrane Proteomics ◽  
Specific Protocol ◽  
Set Up

Set-up of a specific protocol for membrane protein analysis, applied to label free, comparative proteomics of renal cell carcinoma microdomains.

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