Diagnosis of thymic epithelial tumor subtypes by a quantitative proteomic approach

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2491-2500 ◽  
Author(s):  
Ting Zhao ◽  
Jie Wu ◽  
Xiaohui Liu ◽  
Lei Zhang ◽  
Gang Chen ◽  
...  
Keyword(s):  
Proteomic Profiling ◽  
Epithelial Tumor ◽  
Tumor Subtypes ◽  
Thymic Epithelial Tumor ◽  
Proteomic Approach

This study shows the first depth proteomic profiling of all TET subtypes and six candidate biomarkers were identified and validated.

scholarly journals P1.17-019 B7-H3 Protein Expression in Thymic Epithelial Tumor Subtypes and Its Association with PD-L1 and Clinical Characteristics

2017 ◽  
Vol 12 (11) ◽  
pp. S2067-S2068
Author(s):  
S. Gennatas ◽  
A. Mandal ◽  
J. Robertus ◽  
A. Bowman ◽  
A. Nicholson ◽  
...  
Keyword(s):  
Protein Expression ◽  
Clinical Characteristics ◽  
Epithelial Tumor ◽  
Tumor Subtypes ◽  
Thymic Epithelial Tumor

scholarly journals Tumor immunity is related to 18 F‐FDG uptake in thymic epithelial tumor

2021 ◽  
Author(s):  
Hisao Imai ◽  
Kyoichi Kaira ◽  
Kosuke Hashimoto ◽  
Hiroyuki Nitanda ◽  
Ryo Taguchi ◽  
...  
Keyword(s):  
Tumor Immunity ◽  
Epithelial Tumor ◽  
Fdg Uptake ◽  
Thymic Epithelial Tumor

scholarly journals Proteomic profiling of mitochondrial-derived vesicles in brain reveals enrichment of respiratory complex sub-assemblies and small TIM chaperones

2020 ◽  
Author(s):  
Rosalind F. Roberts ◽  
Andrew N. Bayne ◽  
Thomas Goiran ◽  
Dominique Lévesque ◽  
François-Michel Boisvert ◽  
...  
Keyword(s):  
Protein Transport ◽  
Disease Genes ◽  
Proteomic Profiling ◽  
Cell Functions ◽  
Peroxisomal Biogenesis ◽  
Proteomic Approach ◽  
Significant Difference ◽  
Vital Cell ◽  
The Brain

ABSTRACTThe generation of mitochondrial-derived vesicles (MDVs) is implicated in a plethora of vital cell functions, from mitochondrial quality control to peroxisomal biogenesis. The discovery of distinct subtypes of MDVs has revealed the selective inclusion of mitochondrial cargo in response to varying stimuli. However, the true scope and variety of MDVs is currently unclear, and unbiased approaches have yet to be used to understand their biology. Furthermore, as mitochondrial dysfunction has been implicated in many neurodegenerative diseases, it is essential to understand MDV pathways in the nervous system. To address this, we sought to identify the cargo in brain MDVs. We used an in vitro budding assay and proteomic approach to identify proteins selectively enriched in MDVs. 72 proteins were identified as MDV enriched, of which 31% were OXPHOS proteins. Interestingly, the OXPHOS proteins localized to specific modules of the respiratory complexes, hinting at the inclusion of sub-assemblies in MDVs. Small TIM chaperones were also highly enriched in MDVs, linking mitochondrial chaperone-mediated protein transport to MDV formation. As the two Parkinson’s disease genes PINK1 and Parkin have been previously implicated in MDV biogenesis in response to oxidative stress, we compared the MDV proteomes from the brains of wild-type mice with those of PINK1-/- and Parkin-/- mice. No significant difference was found, suggesting that PINK1- and Parkin-dependent MDVs make up a small proportion of all MDVs in the brain. Our findings demonstrate a previously uncovered landscape of MDV complexity and provide a foundation from which to discover further novel MDV functions.

scholarly journals Comparison of Proteomic Responses as Global Approach to Antibiotic Mechanism of Action Elucidation

2020 ◽  
Vol 65 (1) ◽  
pp. e01373-20
Author(s):  
Christoph H. R. Senges ◽  
Jennifer J. Stepanek ◽  
Michaela Wenzel ◽  
Nadja Raatschen ◽  
Ümran Ay ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Mechanisms Of Action ◽  
Proteomic Profiling ◽  
Content Type ◽  
Bacterial Physiology ◽  
Proteomic Approach ◽  
Promising Target ◽  
New Antibiotics ◽  
Proteomic Responses ◽  
The Impact

ABSTRACTNew antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of the bottlenecks is the elucidation of targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for the rapid classification of compounds into those with precedented and unprecedented modes of action. We established a proteomic response library of Bacillus subtilis covering 91 antibiotics and comparator compounds, and a mathematical approach was developed to aid data analysis. Comparison of proteomic responses (CoPR) allows the rapid identification of antibiotics with dual mechanisms of action as shown for atypical tetracyclines. It also aids in generating hypotheses on mechanisms of action as presented for salvarsan (arsphenamine) and the antirheumatic agent auranofin, which is under consideration for repurposing. Proteomic profiling also provides insights into the impact of antibiotics on bacterial physiology through analysis of marker proteins indicative of the impairment of cellular processes and structures. As demonstrated for trans-translation, a promising target not yet exploited clinically, proteomic profiling supports chemical biology approaches to investigating bacterial physiology.

scholarly journals P1-125: Aberrant methylation of DAP-K, p-16, MGMT, RAR-β and HPP1 in thymic epithelial tumor

2007 ◽  
Vol 2 (8) ◽  
pp. S600
Author(s):  
Yukiko Hirose ◽  
Kazuya Kondo ◽  
Taeko Nagao ◽  
Hiroaki Toba ◽  
Takanori Miyoshi ◽  
...  
Keyword(s):  
Aberrant Methylation ◽  
Epithelial Tumor ◽  
Thymic Epithelial Tumor ◽  
P 16

Paraneoplastic myasthenia gravis: Detection of anti-MGT30 (titin) antibodies predicts thymic epithelial tumor

Neurology ◽  
1997 ◽  
Vol 49 (5) ◽  
pp. 1454-1457 ◽  
Author(s):  
R. D. Voltz ◽  
W. C. Albrich ◽  
A. Nägele ◽  
F. Schumm ◽  
M. Wick ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Predictive Value ◽  
Striated Muscle ◽  
Immunofluorescence Assay ◽  
Epithelial Tumor ◽  
Thymic Epithelial Tumors ◽  
Thymic Epithelial Tumor ◽  
Antibody Assays ◽  
Better Than ◽  
Titin Antibodies

It has been suggested that antibodies against nonacetylcholine receptor proteins of striated muscle are markers of the presence of a thymic epithelial tumor in patients with myasthenia gravis (MG). These antibodies may be measured using an immunofluorescence assay against striated muscle(anti-STR) or an ELISA with a recombinant 30-kd titin fragment (anti-MGT30). To directly compare anti-STR with anti-MGT30, we examined the sera of 276 consecutive patients with known or suspected MG. Definite diagnoses and thymic histology, if available, were correlated with the antibody assays. Of the 276 patients, 164 had MG. Thymic histology was obtained in 44 patients: 18 had lymphofollicular hyperplasia, 13 thymic epithelial tumors, 8 atrophy, and 5 were normal. When compared with anti-STR, anti-MGT30 showed a sensitivity of 69% (STR 77%), specificity of 100% (STR 56%, p = 0.026), negative predictive value of 82% (STR 77%), and positive predictive value of 100% (STR 56%, p = 0.003) for the identification of a thymic epithelial tumor versus thymic hyperplasia. We conclude that the anti-MGT30 ELISA is better than the anti-STR immunofluorescence assay for the diagnosis of paraneoplastic MG.

scholarly journals Perplexing Histologic Classification of Thymic Epithelial Tumor

Radiology ◽  
2015 ◽  
Vol 275 (3) ◽  
pp. 929-930 ◽  
Author(s):  
Qijun Shen ◽  
Wenchao Hu ◽  
Zhan Feng
Keyword(s):  
Epithelial Tumor ◽  
Histologic Classification ◽  
Thymic Epithelial Tumor
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