In situ synthesis of photoluminescence-quenching nanopaper for rapid and robust detection of pathogens and proteins

2019 ◽  
Vol 55 (18) ◽  
pp. 2660-2663 ◽  
Author(s):  
Yucheng Liu ◽  
Guobin Mao ◽  
Wang Wang ◽  
Songbai Tian ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
In Situ Synthesis ◽  
Photoluminescence Quenching ◽  
Robust Detection ◽  
Transfer Protein ◽  
Long Term Storage ◽  
Electron Transfer Protein ◽  
Rapid Reaction ◽  
Term Storage

In this work, the electron transfer protein cytochrome c is innovatively embedded into cellulose paper to prepare photoluminescence-quenching nanopaper with a highly-efficient quenching ability, rapid reaction time and long-term storage.

scholarly journals Effects of Long-Term Storage on the Detection of Proteins, DNA, and mRNA in Tissue Microarray Slides

2011 ◽  
Vol 59 (12) ◽  
pp. 1113-1121 ◽  
Author(s):  
Christina Karlsson ◽  
Mats G. Karlsson
Keyword(s):  
Gene Copy Number ◽  
Tissue Microarrays ◽  
Storage Conditions ◽  
Gene Copy ◽  
Histological Techniques ◽  
Long Term Storage ◽  
Term Storage ◽  
Formalin Fixed

Storage of tissue slides has been claimed to induce dramatically reduced antigen detection particularly for immunohistochemistry (IHC). With tissue microarrays, the necessity to serially cut blocks in order to obtain as much material as possible is obvious. The presumed adverse effect of storage might hamper such an approach. The authors designed an experimental setting consisting of four different storage conditions with storage time of tissue slides of up to 1 year. Detection of proteins, DNA, and mRNA was performed using IHC and in situ hybridization techniques. Slight but significant changes in IHC occurred over time. The most important factor is the primary antibody used: four showed no significant changes, whereas limited decreases in 8 antibodies could be detected by image analysis. Whether the antigen was nuclear or cytoplasmic/membranous did not matter. No major differences between different storage conditions could be shown, but storage at 4C was overall the best procedure. Furthermore, gene copy number aberrations, chromosomal translocations, and the presence of mRNA could be detected on slides stored up to 1 year. In conclusion, in tissues optimally formalin fixed and using modern histological techniques, only minute changes in tissue antigenicity are induced by long-term storage.

Population genetics and conservation of the critically endangered Clematis acerifolia (Ranunculaceae)

2005 ◽  
Vol 83 (10) ◽  
pp. 1248-1256 ◽  
Author(s):  
J. López-Pujol ◽  
F.-M. Zhang ◽  
S. Ge
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Critically Endangered ◽  
Ex Situ ◽  
Genetic Structuring ◽  
Long Term Storage ◽  
Hardy Weinberg Equilibrium ◽  
Term Storage

Allozyme electrophoresis was used to evaluate the levels of genetic diversity and population genetic structure of the critically endangered Clematis acerifolia Maximowicz (Ranunculaceae), a narrow endemic species in China. On the basis of variation at 19 putative loci in nine populations covering the entire distribution of this species, low values of genetic diversity were detected (P = 20.5%, A = 1.27, and He = 0.072). A significant deficiency of heterozygotes was found in all populations. Most loci showed deviations from the Hardy–Weinberg equilibrium, probably as a result of population genetic structuring. The high genetic divergence among populations (FST = 0.273) can be interpreted as an effect of the extinction of local populations and genetic drift within extant populations, and has probably been enhanced by habitat fragmentation in recent decades. Threats to this species are mainly anthropogenic (road works, construction of holiday resorts, and extraction activities), although stochastic risks cannot be ignored. Therefore, to preserve extant genetic variation of C. acerifolia, in situ strategies, such as the preservation of its habitat or at least the most diverse populations, and ex situ measures, such as the collection and long-term storage of seeds, should be adopted.

scholarly journals Assessing the efficiency of a “buried wall” barrier in the establishment of near-surface long-term storage and disposal facilities for RW

2021 ◽  
Vol 14 (1) ◽  
pp. 96-105
Author(s):  
V. V. Suskin ◽  
◽  
I. V. Kapyrin ◽  
F. V. Grigorev ◽  
◽  
...  
Keyword(s):  
Near Surface ◽  
Transfer Processes ◽  
Long Term Storage ◽  
Mass Transfer Processes ◽  
High Degree ◽  
The Impact ◽  
Term Storage

The article evaluates the impact of a “buried wall” barrier on the long-term safety during the long-term storage1 or in-situ disposal of nuclear legacy facilities, in particular, industrial reservoirs, as well as during the development of near-surface disposal facilities for radioactive waste (RWDF). For assessment purposes, filtration and mass transfer processes have been numerically modelled in the GeRa code based on a case study of a reference near-surface facility. The study explores in which way the available covering screen affects the dynamics of contaminant spread. It evaluates the sensitivity of the results to the dispersion parameter commonly characterized by a high degree of uncertainty.

scholarly journals RISE microscopy for evaluation of compounded disinfection products

2018 ◽  
Vol 2018 (6) ◽  
pp. 30-33
Author(s):  
Олег Татарчук ◽  
Oleg Tatarchuk
Keyword(s):  
Emergency Preparedness ◽  
Comprehensive Evaluation ◽  
Chemical Characteristics ◽  
Pharmaceutical Samples ◽  
Long Term Storage ◽  
In Situ Generation ◽  
Non Destructive ◽  
Term Storage

Multicomponent powdered disinfection products with in situ generation of biocidal substances are stable in long-term storage and therefore well suited for emergency preparedness stockpiling to use in outbreaks of infectious diseases. Certain structural and chemical characteristics of the powdered biocidal mixtures might alter their efficacy, safety, or handling properties. The RISE microscopy is a new non-destructive method for comprehensive evaluation of pharmaceutical samples by combining confocal Raman imaging with scanning electron microscopy. The RISE microscopy can be used for evaluation of compounded disinfection products.

scholarly journals Recycling and Long-Term Storage of Fluorescence In Situ Hybridization Slides

2014 ◽  
Vol 141 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Susumu Wakai ◽  
Yasuo Shibuki ◽  
Karin Yokozawa ◽  
Shoko Nakamura ◽  
Yuko Adegawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Long Term Storage ◽  
Term Storage

Long‐term storage of forage legumes greatly alters the hard seed breakdown pattern in situ

2020 ◽  
Author(s):  
Robert J. Harrison ◽  
John G. Howieson ◽  
Ron J. Yates ◽  
Bradley J. Nutt
Keyword(s):  
Forage Legumes ◽  
Hard Seed ◽  
Long Term Storage ◽  
Term Storage

ALASKA CLEAN SEAS: MEETING RESPONSE NEEDS FOR 1987 AND BEYOND

1987 ◽  
Vol 1987 (1) ◽  
pp. 145-149
Author(s):  
Richard V. Shafer
Keyword(s):  
Bering Sea ◽  
Petroleum Exploration ◽  
Long Term Storage ◽  
Countermeasures Research ◽  
Reduced Activity ◽  
Term Storage ◽  
Exploration Activity ◽  
The Bering Sea

ABSTRACT The Alaska Clean Seas organization's spill countermeasures research program continues, with recently completed or ongoing projects addressing spill response chemicals, shoreline habitat protection, bubble barriers, water jet barriers, in situ combustion equipment, and under-ice oil detection. Lack of petroleum exploration activity in the Bering Sea has brought about a decision to place response equipment dedicated for that area in long-term storage. Training schedules have also been adjusted because of reduced activity, but program quality is being maintained. Participating companies continue to enjoy an excellent spill safety record in Alaska, and Alaska Clean Seas has not been required to take part in any actual spill cleanup operations.

Synchrotron Diffraction Study of the Isothermal Oxidation of Uranium Dioxide at 250°C

2003 ◽  
Vol 802 ◽  
Author(s):  
Gurvan Rousseau ◽  
Lionel Desgranges ◽  
Jean-Claude Nièpce ◽  
Jean-François Bérar ◽  
Gianguido Baldinozzi
Keyword(s):  
Spent Nuclear Fuel ◽  
Structural Evolution ◽  
Isothermal Oxidation ◽  
Cubic Phase ◽  
X Ray Diffraction ◽  
Long Term Storage ◽  
Instrumental Resolution ◽  
Term Storage

ABSTRACTThe structural evolution of UO2 during its oxidation to U3O8 at 250°C in air was studied by in-situ synchrotron X-ray diffraction on the D2AM-CRG beamline at ESRF. The aim of this study is to determine the phases that are likely to appear during the long-term storage of spent nuclear fuel. Our results are in disagreement with the literature in which the existence of an intermediate cubic phase is not reported. Instead, an α-U3O7 tetragonal phase (c/a < 1) was mentioned but not definitively observed. These previous interpretations may have been the result of poor instrumental resolution.

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