Population genetics and conservation of the critically endangered Clematis acerifolia (Ranunculaceae)

2005 ◽  
Vol 83 (10) ◽  
pp. 1248-1256 ◽  
Author(s):  
J. López-Pujol ◽  
F.-M. Zhang ◽  
S. Ge
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Critically Endangered ◽  
Ex Situ ◽  
Genetic Structuring ◽  
Long Term Storage ◽  
Hardy Weinberg Equilibrium ◽  
Term Storage

Allozyme electrophoresis was used to evaluate the levels of genetic diversity and population genetic structure of the critically endangered Clematis acerifolia Maximowicz (Ranunculaceae), a narrow endemic species in China. On the basis of variation at 19 putative loci in nine populations covering the entire distribution of this species, low values of genetic diversity were detected (P = 20.5%, A = 1.27, and He = 0.072). A significant deficiency of heterozygotes was found in all populations. Most loci showed deviations from the Hardy–Weinberg equilibrium, probably as a result of population genetic structuring. The high genetic divergence among populations (FST = 0.273) can be interpreted as an effect of the extinction of local populations and genetic drift within extant populations, and has probably been enhanced by habitat fragmentation in recent decades. Threats to this species are mainly anthropogenic (road works, construction of holiday resorts, and extraction activities), although stochastic risks cannot be ignored. Therefore, to preserve extant genetic variation of C. acerifolia, in situ strategies, such as the preservation of its habitat or at least the most diverse populations, and ex situ measures, such as the collection and long-term storage of seeds, should be adopted.

scholarly journals Effects of Long-Term Storage on the Detection of Proteins, DNA, and mRNA in Tissue Microarray Slides

2011 ◽  
Vol 59 (12) ◽  
pp. 1113-1121 ◽  
Author(s):  
Christina Karlsson ◽  
Mats G. Karlsson
Keyword(s):  
Gene Copy Number ◽  
Tissue Microarrays ◽  
Storage Conditions ◽  
Gene Copy ◽  
Histological Techniques ◽  
Long Term Storage ◽  
Term Storage ◽  
Formalin Fixed

Storage of tissue slides has been claimed to induce dramatically reduced antigen detection particularly for immunohistochemistry (IHC). With tissue microarrays, the necessity to serially cut blocks in order to obtain as much material as possible is obvious. The presumed adverse effect of storage might hamper such an approach. The authors designed an experimental setting consisting of four different storage conditions with storage time of tissue slides of up to 1 year. Detection of proteins, DNA, and mRNA was performed using IHC and in situ hybridization techniques. Slight but significant changes in IHC occurred over time. The most important factor is the primary antibody used: four showed no significant changes, whereas limited decreases in 8 antibodies could be detected by image analysis. Whether the antigen was nuclear or cytoplasmic/membranous did not matter. No major differences between different storage conditions could be shown, but storage at 4C was overall the best procedure. Furthermore, gene copy number aberrations, chromosomal translocations, and the presence of mRNA could be detected on slides stored up to 1 year. In conclusion, in tissues optimally formalin fixed and using modern histological techniques, only minute changes in tissue antigenicity are induced by long-term storage.

scholarly journals Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Shidong Yue ◽  
Yu Zhang ◽  
Yi Zhou ◽  
Shaochun Xu ◽  
Shuai Xu ◽  
...  
Keyword(s):  
Seed Storage ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Ex Situ ◽  
Zostera Japonica ◽  
Storage Method ◽  
Seagrass Meadows ◽  
Long Term Storage ◽  
Term Storage

Abstract Background Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as – 5 °C. However, our results indicated that reduced storage temperature to 0 °C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 °C under a salinity of 40–60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 °C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 ± 16.8%. Conclusion Our experiments demonstrated that seeds stored at 0 °C under a salinity of 40–60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.

scholarly journals Assessing the efficiency of a “buried wall” barrier in the establishment of near-surface long-term storage and disposal facilities for RW

2021 ◽  
Vol 14 (1) ◽  
pp. 96-105
Author(s):  
V. V. Suskin ◽  
◽  
I. V. Kapyrin ◽  
F. V. Grigorev ◽  
◽  
...  
Keyword(s):  
Near Surface ◽  
Transfer Processes ◽  
Long Term Storage ◽  
Mass Transfer Processes ◽  
High Degree ◽  
The Impact ◽  
Term Storage

The article evaluates the impact of a “buried wall” barrier on the long-term safety during the long-term storage1 or in-situ disposal of nuclear legacy facilities, in particular, industrial reservoirs, as well as during the development of near-surface disposal facilities for radioactive waste (RWDF). For assessment purposes, filtration and mass transfer processes have been numerically modelled in the GeRa code based on a case study of a reference near-surface facility. The study explores in which way the available covering screen affects the dynamics of contaminant spread. It evaluates the sensitivity of the results to the dispersion parameter commonly characterized by a high degree of uncertainty.

Critical review of threatened species collections in the New South Wales Seedbank: implications for ex situ conservation of biodiversity

2004 ◽  
Vol 10 (4) ◽  
pp. 221 ◽  
Author(s):  
C. A. Offord ◽  
M. L. McKensy ◽  
P. V. Cuneo
Keyword(s):  
Moisture Content ◽  
Threatened Species ◽  
New South ◽  
Ex Situ ◽  
Seed Moisture Content ◽  
Long Term Storage ◽  
South Wales ◽  
Seed Moisture ◽  
Term Storage

This article reviews the germinability and viability of seeds of threatened species collections in the New South Wales (NSW) Seedbank with the manifold aims of: ensuring that existing storage treatments and conditions provide effective ex situ storage of threatened species seed; providing baseline viability and seed storage life data on threatened species; and, identifying research gaps in seed germination and storage protocols for threatened species and communities. The germinability and viability of a range of seed accessions, of various ages and stored under different (although mainly identifiable) conditions in the NSW Seedbank, was determined through germination and cut-tests. The results indicated that many of the Fabaceae, Myrtaceae and Proteaceae species tested are orthodox and can be stored at 5 to 10% moisture content at 5�C for up to ten years without significant loss of viability (short- to medium-term storage). The best results were obtained in the lower seed moisture content range (2 to 9%), which appeared to be especially critical for long-term storage of many Proteaceae accessions, reinforcing the need to attain the correct seed moisture content for long-term storage. Around 10% of accessions exhibited some degree of dormancy even after long storage periods. Storage of the widest range of species, for periods greater than ten years for long-term conservation purposes, is generally best conducted by storing at sub-zero temperatures. Freezing at -18�C had little effect on the germinability of a range of seeds tested and is recommended over storage at 5�C. Collection and seed banking procedures for the NSW Seedbank will be regularly reviewed and procedures modified in order to identify the best long-term storage conditions for species within this and other seedbanks. Seed collection strategies to maximize diversity and uses of seedbanks in conservation are discussed.

scholarly journals RISE microscopy for evaluation of compounded disinfection products

2018 ◽  
Vol 2018 (6) ◽  
pp. 30-33
Author(s):  
Олег Татарчук ◽  
Oleg Tatarchuk
Keyword(s):  
Emergency Preparedness ◽  
Comprehensive Evaluation ◽  
Chemical Characteristics ◽  
Pharmaceutical Samples ◽  
Long Term Storage ◽  
In Situ Generation ◽  
Non Destructive ◽  
Term Storage

Multicomponent powdered disinfection products with in situ generation of biocidal substances are stable in long-term storage and therefore well suited for emergency preparedness stockpiling to use in outbreaks of infectious diseases. Certain structural and chemical characteristics of the powdered biocidal mixtures might alter their efficacy, safety, or handling properties. The RISE microscopy is a new non-destructive method for comprehensive evaluation of pharmaceutical samples by combining confocal Raman imaging with scanning electron microscopy. The RISE microscopy can be used for evaluation of compounded disinfection products.

scholarly journals Recycling and Long-Term Storage of Fluorescence In Situ Hybridization Slides

2014 ◽  
Vol 141 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Susumu Wakai ◽  
Yasuo Shibuki ◽  
Karin Yokozawa ◽  
Shoko Nakamura ◽  
Yuko Adegawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Long Term Storage ◽  
Term Storage

Long‐term storage of forage legumes greatly alters the hard seed breakdown pattern in situ

2020 ◽  
Author(s):  
Robert J. Harrison ◽  
John G. Howieson ◽  
Ron J. Yates ◽  
Bradley J. Nutt
Keyword(s):  
Forage Legumes ◽  
Hard Seed ◽  
Long Term Storage ◽  
Term Storage

In situ synthesis of photoluminescence-quenching nanopaper for rapid and robust detection of pathogens and proteins

2019 ◽  
Vol 55 (18) ◽  
pp. 2660-2663 ◽  
Author(s):  
Yucheng Liu ◽  
Guobin Mao ◽  
Wang Wang ◽  
Songbai Tian ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
In Situ Synthesis ◽  
Photoluminescence Quenching ◽  
Robust Detection ◽  
Transfer Protein ◽  
Long Term Storage ◽  
Electron Transfer Protein ◽  
Rapid Reaction ◽  
Term Storage

In this work, the electron transfer protein cytochrome c is innovatively embedded into cellulose paper to prepare photoluminescence-quenching nanopaper with a highly-efficient quenching ability, rapid reaction time and long-term storage.

ALASKA CLEAN SEAS: MEETING RESPONSE NEEDS FOR 1987 AND BEYOND

1987 ◽  
Vol 1987 (1) ◽  
pp. 145-149
Author(s):  
Richard V. Shafer
Keyword(s):  
Bering Sea ◽  
Petroleum Exploration ◽  
Long Term Storage ◽  
Countermeasures Research ◽  
Reduced Activity ◽  
Term Storage ◽  
Exploration Activity ◽  
The Bering Sea

ABSTRACT The Alaska Clean Seas organization's spill countermeasures research program continues, with recently completed or ongoing projects addressing spill response chemicals, shoreline habitat protection, bubble barriers, water jet barriers, in situ combustion equipment, and under-ice oil detection. Lack of petroleum exploration activity in the Bering Sea has brought about a decision to place response equipment dedicated for that area in long-term storage. Training schedules have also been adjusted because of reduced activity, but program quality is being maintained. Participating companies continue to enjoy an excellent spill safety record in Alaska, and Alaska Clean Seas has not been required to take part in any actual spill cleanup operations.

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