Self-assembled nano-aggregates of fluorinases demonstrate enhanced enzymatic activity, thermostability and reusability

2020 ◽  
Vol 8 (2) ◽  
pp. 648-656
Author(s):  
Chunhao Tu ◽  
Jin Zhou ◽  
Lei Peng ◽  
Shuli Man ◽  
Long Ma

Three SAP (self-assembling peptide)-tagged fluorinases (FLAs) are successfully prepared. All three SAP-tagged FLAs bear enzymatic activity and they form nano-sized particles in aqueous solution. One of them displays improved enzyme activity, thermostability and reusability.

2021 ◽  
Author(s):  
Lifang Qi ◽  
Yao Le ◽  
Chao Wang ◽  
Rui Lei ◽  
Tian Wu

Self-assembling ultrathin active δ-MnO2 nanosheets and Mn3O4 octahedrons into hierarchical texture enhances room-temperature formaldehyde oxidation at a low-level of Pt.


Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Sebastian Dahle ◽  
John Meuthen ◽  
René Gustus ◽  
Alexandra Prowald ◽  
Wolfgang Viöl ◽  
...  

Self-assembling films typically used for colloidal lithography have been applied to pine wood substrates to change the surface wettability. Therefore, monodisperse polystyrene (PS) spheres have been deposited onto a rough pine wood substrate via dip coating. The resulting PS sphere film resembled a polycrystalline face centered cubic (FCC)-like structure with typical domain sizes of 5–15 single spheres. This self-assembled coating was further functionalized via an O2 plasma. This plasma treatment strongly influenced the particle sizes in the outermost layer, and hydroxyl as well as carbonyl groups were introduced to the PS spheres’ surfaces, thus generating a superhydrophilic behavior.


2003 ◽  
Vol 15 (12) ◽  
pp. 2469-2476 ◽  
Author(s):  
Y. Masuda ◽  
T. Sugiyama ◽  
W. S. Seo ◽  
K. Koumoto

2018 ◽  
Vol 20 (1) ◽  
pp. 422-434
Author(s):  
Kyle M. Koss ◽  
Christopher Tsui ◽  
Larry D. Unsworth

Genetics ◽  
1980 ◽  
Vol 94 (2) ◽  
pp. 327-339 ◽  
Author(s):  
Richard Snow

ABSTRACT The HIS1 and THR4 loci are the structural genes for phosphoribosyl-ATP pyrophosphorylase and threonine synthetase, respectively. The allele his1-IS has no enzyme activity at 30", but does have activity at 15" provided the cell contains the wild-type THR4 allele or a suppressing allele at another locus, designated SUP(his1-1S). Under these conditions, cells with the hisl-IS mutation are capable of growth on minimal medium at 15". Three kinds of reversions of a hisl-IS thr4 sup(his1-IS) strain to histidine prototrophy have been obtained: (1) his1-IS locus reversions to HIS1 that restore growth without added histidine at 30", (2)  thr4 reversions to THR4 that simultaneously eliminate the requirement for threonine and restore the low-temperature effect on the his1-IS allele, and (3)mutations from sup to SUP. The SUP allele is not an ochre suppressor, and it is not linked to either HISI, THR4 or a centromere. It may represent a missense suppressor. I t is proposed that the effect ofTHR4 is caused by aggregation of the wild-type threonine synthetase with defective his1-IS monomers, causing a favorable conformational change in the histidine protein that restores limited enzymatic activity. This can be regarded as a case of complementation between nonhomologous proteins.


2021 ◽  
Vol 6 (1) ◽  
pp. 33-42
Author(s):  
Xiaona Chen ◽  
Zhen Hu ◽  
Liqian Zhou ◽  
Fu Zhang ◽  
Jianqin Wan ◽  
...  

Self-assembled dasatinib nanoparticles exhibited aggregation-induced emission with high antitumor activity.


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