The micelles of poly(styrene-b-3-(methacryloylamino)propyltrimethylammonium chloride-b-ethylene oxide) (PS-b-PMAPTAC-b-PEO) have been successfully prepared in aqueous solutions. The micelles have a PS core, cationic PMAPTAC shell, and PEO corona. Due to the short PS chain (degree of polymerization = 8), the formation of micelles is difficult at a low concentration, and the micelles are detected only at concentrations higher than 1 g L–1. The addition of anionic amphiphiles, such as sodium dodecyl sulfate (SDS) and poly(methacrylic acid) (PMAA), induces the formation of mixed micelles at a low concentration level of the polymer (~0.005 g L–1). This can be ascribed to insolubilization of the cationic PMAPTAC block due to charge neutralization by the anionic amphiphiles. The binding of SDS or PMAA to the PMAPTAC block is confirmed by zeta-potential measurements. The mixed micelles are characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), and fluorescence measurements. Based on DLS measurements, it is revealed that the hydrodynamic diameter of the mixed micelles falls in the range of 120–130 nm. SEM measurements provided clear pictures of mixed micelles with a spherical morphology. The kinetics of exchange of organic dyes between the micelle particles was investigated by fluorescence techniques. The result indicates that the exchange of the dyes between the micelle particles takes places within a time scale of seconds.