scholarly journals UPLC-QTOF-MS-guided isolation of anti-COPD ginsenosides from wild ginseng

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38658-38668
Author(s):  
Hailin Zhu ◽  
Junli Liu ◽  
Hongqiang Lin ◽  
Ying Zhang ◽  
Na Yang ◽  
...  
Keyword(s):  
Fractionation Procedure ◽  
Wild Ginseng

Four previously undescribed ginsenosides, along with five known analogues were isolated from wild ginseng by a UPLC-QTOF-MS-guided fractionation procedure.

An Evaluation of a Plasma Fractionation System

1960 ◽  
Vol 4 (01) ◽  
pp. 031-044
Author(s):  
George Y. Shinowara ◽  
E. Mary Ruth
Keyword(s):  
Biological Activity ◽  
Ionic Strength ◽  
Plasma Proteins ◽  
High Concentration ◽  
Significant Evidence ◽  
Native Proteins ◽  
Mobility Data ◽  
Fractionation Procedure ◽  
The Individual ◽  
Low Ionic Strength

SummaryFour primary fractions comprising at least 97 per cent of the plasma proteins have been critically appraised for evidence of denaturation arising from a low temperature—low ionic strength fractionation system. The results in addition to those referable to the recovery of mass and biological activity include the following: The high solubilities of these fractions at pH 7.3 and low ionic strengths; the compatibility of the electrophoretic and ultracentrifugal data of the individual fractions with those of the original plasma; and the recovery of hemoglobin, not hematin, in fraction III obtained from specimens contaminated with this pigment. However, the most significant evidence for minimum alterations of native proteins was that the S20, w and the electrophoretic mobility data on the physically recombined fractions were identical to those found on whole plasma.The fractionation procedure examined here quantitatively isolates fibrinogen, prothrombin and antithrombin in primary fractions. Results have been obtained demonstrating its significance in other biological systems. These include the following: The finding of 5 S20, w classes in the 4 primary fractions; the occurrence of more than 90 per cent of the plasma gamma globulins in fraction III; the 98 per cent pure albumin in fraction IV; and, finally, the high concentration of beta lipoproteins in fraction II.

scholarly journals 177 Saponins, Including 11 New Compounds in Wild Ginseng Tentatively Identified via HPLC-IT-TOF-MSn, and Differences among Wild Ginseng, Ginseng under Forest, and Cultivated Ginseng

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3371
Author(s):  
Chao-Qun Wang ◽  
Li-Wei Yi ◽  
Lin Zhao ◽  
Yu-Zhen Zhou ◽  
Fang Guo ◽  
...  
Keyword(s):  
Natural Environment ◽  
Mountain Forest ◽  
Characteristic Peak ◽  
Chromatographic Fingerprints ◽  
New Compounds ◽  
First Time ◽  
Peak Pattern ◽  
Wild Ginseng

Wild ginseng (W-GS), ginseng under forest (F-GS, planted in mountain forest and growing in natural environment), and cultivated ginseng (C-GS) were compared via HPLC-DAD and HPLC-IT-TOF-MSn. A total of 199 saponins, including 16 potential new compounds, were tentatively identified from 100 mg W-GS (177 saponins in W-GS with 11 new compounds), F-GS (56 saponins with 1 new compound), and C-GS (60 saponins with 6 new compounds). There were 21 saponins detected from all the W-GS, F-GS, and C-GS. Fifty saponins were only detected from W-GS, including 23 saponins found in ginseng for the first time. Contents of ginsenosides Re (12.36–13.91 mg/g), Rh1 (7.46–7.65 mg/g), Rd (12.94–12.98 mg/g), and the total contents (50.52–55.51 mg/g) of Rg1, Re, Rf, Rb1, Rg2, Rh1, and Rd in W-GS were remarkably higher than those in F-GS (Re 1.22–3.50 mg/g, Rh1 0.15–1.49 mg/g, Rd 0.19–1.49 mg/g, total 5.69–18.74 mg/g), and C-GS (Re 0.30–3.45 mg/g, Rh1 0.05–3.42 mg/g, Rd 0.17–1.68 mg/g, total 2.99–19.55 mg/g). Contents of Re and Rf were significantly higher in F-GS than those in C-GS (p < 0.05). Using the contents of Re, Rf, or Rb1, approximately a half number of cultivated ginseng samples could be identified from ginseng under forest. Contents of Rg1, Re, Rg2, Rh1, as well as the total contents of the seven ginsenosides were highest in ginseng older than 15 years, middle–high in ginseng between 10 to 15 years old, and lowest in ginseng younger than 10 years. Contents of Rg1, Re, Rf, Rb1, Rg2, and the total of seven ginsenosides were significantly related to the growing ages of ginseng (p < 0.10). Similarities of chromatographic fingerprints to W-GS were significantly higher (p < 0.05) for F-GS (median: 0.824) than C-GS (median: 0.745). A characteristic peak pattern in fingerprint was also discovered for distinguishing three types of ginseng. Conclusively, wild ginseng was remarkably superior to ginseng under forest and cultivated ginseng, with ginseng under forest slightly closer to wild ginseng than cultivated ginseng. The differences among wild ginseng, ginseng under forest, and cultivated ginseng in saponin compositions and contents of ginsenosides were mainly attributed to their growing ages.

The potent protective effect of wild ginseng (Panax ginseng C.A. Meyer) against benzo[α]pyrene-induced toxicity through metabolic regulation of CYP1A1 and GSTs

2007 ◽  
Vol 112 (3) ◽  
pp. 568-576 ◽  
Author(s):  
Sang Il Gum ◽  
Sung Jun Jo ◽  
Sang Hyun Ahn ◽  
Sang Geon Kim ◽  
Jin-Taek Kim ◽  
...  
Keyword(s):  
Protective Effect ◽  
Panax Ginseng ◽  
Metabolic Regulation ◽  
Wild Ginseng

CHANGES WITH TIME IN THE FORM AND AVAILABILITY OF RESIDUAL FERTILIZER PHOSPHORUS ON CHERNOZEMIC SOILS

1986 ◽  
Vol 66 (1) ◽  
pp. 105-119 ◽  
Author(s):  
B. I. WAGAR ◽  
J. W. B. STEWART ◽  
J. O. MOIR
Keyword(s):  
Key Words ◽  
Organic P ◽  
Clay Loam ◽  
P Fractionation ◽  
Residual P ◽  
Fractionation Procedure ◽  
P Fertilizer ◽  
Labile P ◽  
P Transformations ◽  
Stable Forms

A sequential phosphorus (P) fractionation procedure was used to measure the changes in the labile and stable forms of inorganic and organic P following single broadcast P applications to Canadian Chernozemic soils under cereal cropping. Approximately half of the fertilizer residues remained in plant-available forms (resin, NaHCO3). In a Black Waskada clay loam 8 yr after the application of 200 and 400 kg P ha−1, residual fertilizer P consisted of resin-P, 30–40%; HCl-P, 25–30%; residue-P, 10–15%; NaOH-P, 10–15%, NaHCO3-P, 10%; and aggregate protected P, 3%. The residues in a Dark Brown Sutherland clay 5 yr after the application of 160 kg P ha−1 were: resin-P, 35%; NaOH-P, 30–40%; NaHCO3-P, 15%; HCl-P, 0–5%; H2SO4-P, 5%; and aggregate protected P, 5%. The soils differed in the quantity of fertilizer recovered in inorganic HCl-extractable forms. In the Sutherland soil the change from wheat-fallow to continuous wheat cropping produced a build-up of organic P which occurred with and without the addition of P fertilizer. Key words: Residual P, P transformations, Labile Pi; labile Po, stable Pi stable Po

Relationship between phosphorus fractions and properties of highly calcareous soils

Soil Research ◽  
2007 ◽  
Vol 45 (4) ◽  
pp. 255 ◽  
Author(s):  
Ebrahim Adhami ◽  
Hamid Reza Memarian ◽  
Farzad Rassaei ◽  
Ehsan Mahdavi ◽  
Manouchehr Maftoun ◽  
...  
Keyword(s):  
Calcareous Soils ◽  
Hydroxylamine Hydrochloride ◽  
Inorganic Phosphorus ◽  
Influential Factor ◽  
Sequential Fractionation ◽  
Inorganic P ◽  
Soil P ◽  
Fractionation Procedure ◽  
Extractable P ◽  
P Content

Inorganic phosphorus (P) sequential fractionation schemes are applicable techniques to interpret soil P status. The present study was initiated to determine the origin of various P fractions in highly calcareous soils. Inorganic P forms were determined by a sequential fractionation procedure extracting with NaOH (NaOH-P), Na citrate-bicarbonate (CB-P), Na citrate 2 times (C1-P and C2-P), Na citrate-ascorbate (CAs-P), Na citrate-bicarbonate-dithionite (CBD-P), Na acetate (NaAc-P), and HCl (HCl-P). Results showed that NaOH-P was negatively correlated with active iron oxides. CB-P was positively correlated with silt content and negatively related to citrate-bicarbonate-dithionite extractable Fe (Fed). This result illustrates the weathering effect on Ca-P, with Ca-P content declining as a consequence of weathering. A negative correlation was observed between C1-P and citrate ascorbate extractable Fe (FeCAs). Second citrate extractable P (C2-P) was negatively related to calcium carbonate equivalent and positively related to hydroxylamine-hydrochloride and neutral ammonium acetate-hydroquinone extractable Mn (Mnh and Mnq). Fine silt (Fsilt) was the most influential factor affecting CAs-P. It seemed citrate-dithionite-bicarbonate extractable Al (Ald), Mnh, and Mnq have been sinks for CBD-P, while free iron oxide compounds (Feo, Fec, and FeCAs) were a major contributing factor for the formation of NaAc-P. Stable P compounds (HCl-P) of highly calcareous soils originated from coarse silt (Csilt) and hydroxylamine-hydrochloride extractable Mn (Mnh).

scholarly journals Halobacterium cutirubrum ribosomes. Properties of the ribosomal proteins and ribonucleic acid

1972 ◽  
Vol 130 (1) ◽  
pp. 103-110 ◽  
Author(s):  
L. P. Visentin ◽  
C. Chow ◽  
A. T. Matheson ◽  
M. Yaguchi ◽  
F. Rollin
Keyword(s):  
Ribosomal Protein ◽  
Ribosomal Proteins ◽  
Soluble Fraction ◽  
Ribosomal Subunit ◽  
23S Rrna ◽  
Core Particle ◽  
Fractionation Procedure ◽  
Additional Protein ◽  
70S Ribosome ◽  
Low Ionic Strength

1. The 30S ribosomal subunit of the extreme halophile Halobacterium cutirubrum is unstable and loses 75% of its ribosomal protein when the 70S ribosome is dissociated into the two subunits. A stable 30S subunit is obtained if the dissociation of the 70S particle is carried out in the presence of the soluble fraction. 2. A fractionation procedure was developed for the selective removal of groups of proteins from the 30S and 50S subunits. When the ribosomes, which are stable in 4m-K+ and 0.1m-Mg2+, were extracted with low-ionic-strength buffer 75–80% of the 30S proteins and 60–65% of the 50S proteins as well as the 5S rRNA were released. The proteins in this fraction are the most acidic of the H. cutirubrum ribosomal proteins. Further extraction with Li+–EDTA releases additional protein, leaving a core particle containing either 16S rRNA or 23S rRNA and about 5% of the total ribosomal protein. The amino acid composition, mobility on polyacrylamide gels at pH4.5 and 8.7, and the molecular-weight distribution of the various protein fractions were determined. 3. The s values of the rRNA are 5S, 16S and 23S. The C+G contents of the 16S and 23S rRNA were 56.1 and 58.8% respectively and these are higher than C+G contents of the corresponding Escherichia coli rRNA (53.8 and 54.1%).

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