scholarly journals A FRACTIONATION PROCEDURE FOR THE ACID-SOLUBLE PHOSPHORUS COMPOUNDS OF LIVER

1949 ◽  
Vol 181 (2) ◽  
pp. 655-666 ◽  
Author(s):  
Jacob. Sacks
Keyword(s):  
Phosphorus Compounds ◽  
Fractionation Procedure ◽  
Soluble Phosphorus

Solid, slightly soluble phosphorus compounds as nutrient source in activated sludge treatment of forest industry wastewaters

1997 ◽  
Vol 35 (2-3) ◽  
pp. 131-138 ◽  
Author(s):  
Pirjo-Riitta Rantala ◽  
Hannu Wirola
Keyword(s):  
Activated Sludge ◽  
The Other ◽  
Phosphorus Compounds ◽  
Forest Industry ◽  
Phosphorus Concentration ◽  
Nutrient Source ◽  
Sludge Treatment ◽  
Grain Sizes ◽  
Soluble Phosphorus ◽  
The Mean

The aim of the study was to determine if solid, slightly soluble compounds can be used as nutrient source in activated sludge treatment plants instead of liquid phosphoric acid. Four different solid materials were tested in lab-scale solubility tests to find compounds which are least soluble. Two materials were chosen for further studies: apatite and raw phosphate. The use of apatite and raw phosphate as nutrient source was studied in lab-scale activated sludge reactors along with a control reactor where phosphorus was added in liquid form. The phosphorus dosage, measured as elementary phosphorus, was the same for all three reactors. The reactors were fed with pre-clarified chemi-thermomechanical pulp mill (CTMP) wastewater. There were no significant differences in the reductions of organic matter between the three reactors. The mean effluent concentration of total phosphorus was 3 mg P/l in the control reactor and less than 1 mg P/1 in the other two reactors. The soluble phosphorus concentration was more than 2 mg P/l in the control reactor and less than 0.5 mg P/l in the other two. Apatite was an even better nutrient source than raw phosphate. Further lab-scale tests were conducted using two different grain sizes of apatite. No significant differences were found between the studied grain sizes (<0.074 mm and 0.074 mm-0.125 mm). Apatite was then used in full-scale at a CTMP-mill two different times. The experiments showed that the mean concentrations of phosphorus can be reduced radically by using apatite as a nutrient source instead of liquid phosphorus. Solid phosphorus compounds are a viable alternative to reduce the phosphorus load from forest industry wastewater treatment plants.

scholarly journals The dynamics of acid-soluble phosphorus compounds in the course of winter and spring wheat germination under various thermic conditions. Part II. Labile phosphorus after hydrolysis of the acid-soluble fraction

2015 ◽  
Vol 24 (2) ◽  
pp. 297-308
Author(s):  
A. Barbaro
Keyword(s):  
Low Temperature ◽  
Phosphorus Content ◽  
Soluble Fraction ◽  
Phosphorus Compounds ◽  
Labile Phosphorus ◽  
Soluble Phosphorus ◽  
Germination Temperature ◽  
Wheat Germination ◽  
Labile P ◽  
Hydrolysis Of

The changes in labile phosphorus compounds content during germination of wheat were investigated. These compounds were determined in acid-soluble germ extracts separated into fractions according to the solubility of their barium salts. Low germination temperature was found to raise the labile phosphorus content in the fraction of insoluble barium salts. If we assume that labile P of this fraction consisted mainly of adenosinedi- and triphosphates, it would seem that the rise, in the ATP and ADP level under the influence of low temperature may be essential for initiating flowering in winter varieties.

Acid-Soluble Deoxynucleotides and DNA Synthesis in Growing Yeast after X-Irradiation, II. Synthesis of Deoxynucleoside Tri- and Monophosphates in Synchronized and Asynchronously Growing Cells

1974 ◽  
Vol 29 (5-6) ◽  
pp. 272-282 ◽  
Author(s):  
Hans Eckstein ◽  
Sybille Ahnefeld ◽  
Karin Albietz-Loges
Keyword(s):  
Dna Replication ◽  
Radiation Effects ◽  
Phosphorus Uptake ◽  
Specific Radioactivity ◽  
Phosphorus Compounds ◽  
Pronounced Increase ◽  
Precursor Pool ◽  
Soluble Phosphorus ◽  
X Irradiation ◽  
Quantitative Changes

The behaviour of acid-soluble DNA precursors in synchronized and asynchronously growing yeast after X-irradiation is investigated by labeling techniques with 32Pi and by enzymic estimation. In prelabeled synchronized growing cells, radioactivity associated with deoxynucleoside triphosphates increases to maximum values shortly before each DNA replication, followed by a drastic decrease during S-phase. Radioactivity associated with monophosphates fluctuates, too, but with an opposite rhythm. These fluctuations apparently reflect quantitative changes of the DNA precursor pool during a cell cycle, as judged from the following findings: 1. Acid-soluble phosphorus is augmented stepwise. 2. "Specific" radioactivity from acid-soluble phosphorus compounds decreases steadily, indicating a continuous dilution of the labeled phosphorus pool with "cold" phosphorus. 3. Radioactivity associated with ribonucleotides fluctuates, too, but with a divergent rhythm. In X-irradiated synchronice growing yeast, the fluctuations of the deoxynucleotide-associated 32P are disturbed only little. Maximum values appear nearly at the same time as in the control, they decrease to minor values even if DNA augmentation is delayed. This decrease is less drastic, however, than that during DNA replication in unirradiated yeast, yielding a slightly increased aver­ age label per generation time. At the same sime a rapid augmentation of monophosphate label is observed. A pronounced increase of deoxynucleotide-32P is seen with X-irradiated asynchronously growing yeast, pointing to distinct radiosensitivities of the DNA/DNA precursor system in different cell stages. Neither 32P-fluctuations nor 32P-accumulation during DNA delay can be explained by cor­ responding observations with acid-soluble phosphorus or with ribonucleotide pools. Studies on 32P-incorporation also exclude radiation effects on cellular phosphorus uptake. Enzymic estimations of the deoxynucleoside triphosphate pools from asynchronously growing yeast rather exhibit a considerable increase of these substances during the radiation-induced delay of DNA augmentation. This accumulation of DNA precursors probably is caused by undisturbed synthesis, but reduced incorporation into DNA. The possible role of DNA repair in this system is discussed.

Studies of acid-soluble phosphorus compounds in genetically pure lines of Avena fatua with different dormancy characteristics

1982 ◽  
Vol 60 (10) ◽  
pp. 2099-2104 ◽  
Author(s):  
J. C. Jain ◽  
W. A. Quick ◽  
A. I. Hsiao
Keyword(s):  
Inverse Correlation ◽  
Avena Fatua ◽  
Significant Decline ◽  
Phosphorus Compounds ◽  
Relative Depth ◽  
Uniform Field ◽  
Wild Oat ◽  
Dormancy Breaking ◽  
Soluble Phosphorus ◽  
Pure Lines

Seven genetically pure lines of wild oat selections of known dormancy characteristics were grown under uniform field conditions. Acid-soluble phosphorus components were determined in the dry seeds, in the imbibed seeds, and in imbibed seeds with dormancy-breaking treatments. No significant correlation between dormancy level and acid-soluble phosphorus composition was established for any component other than inorganic phosphorus (Pi). In dry caryopses Pi varied from 5 to 10% of the acid-soluble fraction. A highly significant inverse correlation was found between levels of Pi in the dry caryopsis and the relative depth of dormancy of the wild oat selection considered. The effect of imbibition on Pi level varied with depth of dormancy. Intact seeds of nondormant lines imbibed for 52 h showed a significant decline in Pi prior to coleorhiza protrusion from the dehulled caryopsis. More deeply dormant lines of Avena fatua (pure lines Anderson 51, Anderson 474, or Crop Science 40) required up to 10 days of incubation for significant drops in Pi to appear, while the most dormant lines (pure line Montana 73 and Idaho) did not evidence significant decline in Pi even after 10 days of incubation. The increase in Pi level associated with coleorhiza protrusion could be produced in the imbibed dormant lines by piercing them with a fine needle, by treating with gibberellic acid (GA3), or by a combination of the two treatments. The combination of piercing and GA3 treatment was much more effective than either treatment alone.

scholarly journals Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids

1981 ◽  
Vol 195 (1) ◽  
pp. 301-306 ◽  
Author(s):  
N G Clarke ◽  
R M C Dawson
Keyword(s):  
Water Soluble ◽  
Phosphorus Compounds ◽  
Complete Analysis ◽  
Catalytic Hydrolysis ◽  
Mammalian Tissue ◽  
Fatty Acid Amides ◽  
Phosphorus Containing ◽  
Soluble Phosphorus ◽  
Brain Phospholipids ◽  
Before And After

1. Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.

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