Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3871-3877 ◽  
Author(s):  
Shengda Qi ◽  
Huanhuan Zheng ◽  
Hongyan Qin ◽  
Honglin Zhai
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Alp Activity ◽  
Filter Effect ◽  
Sensitive Method

In this work, a simple and sensitive method based on the inner filter effect (IFE) of p-nitrophenol (PNP) on the fluorescence of gold nanoclusters (AuNCs) has been developed for detecting alkaline phosphatase (ALP) activity.

Water-soluble MoS2 quantum dots for facile and sensitive fluorescence sensing of alkaline phosphatase activity in serum and live cells based on the inner filter effect

Nanoscale ◽  
2018 ◽  
Vol 10 (45) ◽  
pp. 21298-21306 ◽  
Author(s):  
Yaping Zhong ◽  
Fengfeng Xue ◽  
Peng Wei ◽  
Ruohan Li ◽  
Chunyan Cao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Inner Filter Effect ◽  
Water Soluble ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Filter Effect ◽  
Sensitive Method

We report a facile and sensitive method for the detection of alkaline phosphatase (ALP) activity in serum and live cells using molybdenum disulfide quantum dots (MoS2 QDs) based on the Inner Filter Effect (IFE).

In Situ Formation of 2,3-Diaminophenazine for Evaluation of Alkaline Phosphatase Activity via the Inner Filter Effect

2020 ◽  
Vol 3 (9) ◽  
pp. 6394-6399
Author(s):  
Pengjuan Ni ◽  
Siyuan Liu ◽  
Yuanyuan Jiang ◽  
Chuanxia Chen ◽  
Bo Wang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Inner Filter Effect ◽  
Filter Effect ◽  
In Situ Formation

Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cell ◽  
Kinetic Assay ◽  
Human Osteosarcoma ◽  
Alp Activity ◽  
Human Osteosarcoma Cell ◽  
Skeletal Alkaline Phosphatase

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

A MnO2nanosheets–o-phenylenediamine oxidative system for the sensitive fluorescence determination of alkaline phosphatase activity

2018 ◽  
Vol 10 (44) ◽  
pp. 5341-5346 ◽  
Author(s):  
Xionghong Tan ◽  
Zheng Li ◽  
Yanlin Du ◽  
Aixian Zheng ◽  
Yongyi Zeng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alp Activity ◽  
Fluorescence Determination ◽  
Oxidative System

A MnO2nanosheets–o-phenylenediamine (OPDA) oxidative system was developed for detecting ALP activity selectively, sensitively and conveniently.

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