A simplified protein purification method through nickel cleavage of the recombinant protein from the Escherichia coli cell surface

The Analyst ◽  
2020 ◽  
Vol 145 (19) ◽  
pp. 6227-6231
Author(s):  
Shanqing Huang ◽  
Tianbiao Wei ◽  
Wanxing Sha ◽  
Qingyuan Hu ◽  
Yingying Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Cell Surface ◽  
Protein Purification ◽  
Target Protein ◽  
Coli Cell ◽  
Purification Method

A remarkable simple protein purification method is developed through nickel cleaving the target protein off the Escherichia coli cell surface.

scholarly journals A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiao-Feng Zhou ◽  
Chen-Lu Zhang ◽  
Xue-Ping Gao ◽  
Wei-Long Wang ◽  
Zheng-Fen He ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Cell Surface ◽  
Protein Purification ◽  
Surface Display ◽  
Cell Surface Display ◽  
Purification Method ◽  
Ulp1 Protease

scholarly journals Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

2019 ◽  
Vol 116 (6) ◽  
pp. 1259-1268 ◽  
Author(s):  
Martin Lemmerer ◽  
Juergen Mairhofer ◽  
Alexander Lepak ◽  
Karin Longus ◽  
Rainer Hahn ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Functional Expression ◽  
Coli Cell

Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface

2011 ◽  
Vol 12 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Tomohiro Hiraishi ◽  
Koichi Yamashita ◽  
Masafumi Sakono ◽  
Jun Nakanishi ◽  
Liu-Tzea Tan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Coli Cell ◽  
Phb Depolymerase

scholarly journals Growth of the Escherichia coli cell surface.

1977 ◽  
Vol 129 (3) ◽  
pp. 1524-1536 ◽  
Author(s):  
K J Begg ◽  
W D Doanachie
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Coli Cell

Absence of periplasmic DsbA oxidoreductase facilitates export of cysteine-containing passenger proteins to the Escherichia coli cell surface via the Igaβ autotransporter pathway

Gene ◽  
1996 ◽  
Vol 178 (1-2) ◽  
pp. 107-110 ◽  
Author(s):  
Joachim Jose ◽  
Joachim Krämer ◽  
Thomas Klauser ◽  
Johannes Pohlner ◽  
Thomas F. Meyer
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Coli Cell ◽  
Passenger Proteins
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