A green liquid chromatography method for rapid determination of ergosterol in edible fungi based on matrix solid-phase dispersion extraction and a core–shell column

2020 ◽  
Vol 12 (26) ◽  
pp. 3337-3343 ◽  
Author(s):  
Zhengming Qian ◽  
Zi Wu ◽  
Chunhong Li ◽  
Guoying Tan ◽  
Hankun Hu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase ◽  
Rapid Determination ◽  
Edible Fungi ◽  
Chromatography Method ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Liquid Chromatography Method ◽  
Extraction Liquid

A green and rapid matrix solid-phase dispersion extraction-liquid chromatography analytical method for the determination of ergosterol in edible fungi was established.

Rapid Determination of 3 Components With Different Polarities in Medicinal Mushrooms by Multistep Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography Analysis

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098778
Author(s):  
Zhengming Qian ◽  
Zi Wu ◽  
Chunhong Li ◽  
ChangLiang Yao ◽  
Guoying Tan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Rapid Determination ◽  
Chromatography Method ◽  
Medicinal Mushrooms ◽  
Test Range ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion

In this study, a multistep matrix solid-phase dispersion (MSPD) combining with a high-performance liquid chromatography method was developed for assaying 3 components of different polarities (mannitol, adenosine, and ergosterol) from mushroom samples. MSPD extraction was carried out using 1 g octadecyl-bonded silica as the sorbent material, 9% methanol, 20% methanol, and 100% methanol as eluting solvents for the elution of mannitol, adenosine, and ergosterol, respectively. Mannitol was separated on an NH2P-50 4E column and detected using an evaporative light scattering detector. Adenosine and ergosterol were separated on a Poroshell 120 SB-C18 column and measured at 260 nm and 283 nm, respectively. The developed method showed good linearity ( R ≥ 0.9986) within the test range. The relative SD (RSD) of precisions were less than 1.4%, and the recoveries were 95.6%-97.0% (RSD ≤3.0%). Compared with the reported methods, the developed procedure could rapidly prepare components with different polarities (mannitol, adenosine, and ergosterol) from medical mushroom samples with less organic solvent and sample. The method is rapid and eco-friendly, which is helpful to improve the quality evaluation of medicinal mushrooms.

Sign in / Sign up

Export Citation Format

Share Document