Rational design of a lysosome-targeting and near-infrared absorbing Ru(ii)–BODIPY conjugate for photodynamic therapy

2021 ◽  
Vol 57 (14) ◽  
pp. 1790-1793
Author(s):  
Liping Qiao ◽  
Jiangping Liu ◽  
Yunhong Han ◽  
Fangmian Wei ◽  
Xinxing Liao ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Malignant Melanoma ◽  
Rational Design ◽  
Near Infrared ◽  
Inhibitory Efficiency ◽  
A375 Cells

A lysosome-targeting and near-infrared absorbing Ru(ii)–BODIPY conjugate exhibits prominent tumor inhibitory efficiency in malignant melanoma A375 cells and in vivo experiments.

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Folic Acid Functionalized Chlorin e6-Superparamagnetic Iron Oxide Nanocarriers as a Theranostic Agent for MRI-Guided Photodynamic Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Iron Oxide ◽  
Near Infrared ◽  
Biological Fluids ◽  
Superparamagnetic Iron Oxide ◽  
Chlorin E6 ◽  
Theranostic Agent

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.

scholarly journals The Induction of Apoptosis in A375 Malignant Melanoma Cells bySutherlandia frutescens

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Nicola B. van der Walt ◽  
Zahra Zakeri ◽  
Marianne J. Cronjé
Keyword(s):  
Malignant Melanoma ◽  
Basic Mechanism ◽  
Melanoma Cells ◽  
Caspase Activation ◽  
Nuclear Condensation ◽  
Induced Apoptosis ◽  
Apoptosis Inducing Factor ◽  
A375 Melanoma Cell Line ◽  
A375 Cells

Sutherlandia frutescensis a medicinal plant indigenous to Southern Africa and is commonly known as the “cancer bush.” This plant has traditionally been used for the treatment of various ailments, although it is best known for its claims of activity against “internal” cancers. Here we report on its effect on melanoma cells. The aim of this study was to investigate whether an extract ofS. frutescenscould induce apoptosis in the A375 melanoma cell line and to outline the basic mechanism of action.S. frutescensextract induced apoptosis in A375 cells as evidenced by morphological features of apoptosis, phosphatidylserine exposure, nuclear condensation, caspase activation, and the release of cytochromecfrom the mitochondria. Studies in the presence of a pan-caspase inhibitor allude to caspase-independent cell death, which appeared to be mediated by the apoptosis inducing factor. Taken together, the results of this study show thatS. frutescensextract is effective in inducing apoptosis in malignant melanoma cells and indicates that furtherin vivomechanistic studies may be warranted.

scholarly journals A self-assembled Ru–Pt metallacage as a lysosome-targeting photosensitizer for 2-photon photodynamic therapy

2019 ◽  
Vol 116 (41) ◽  
pp. 20296-20302 ◽  
Author(s):  
Zhixuan Zhou ◽  
Jiangping Liu ◽  
Juanjuan Huang ◽  
Thomas W. Rees ◽  
Yiliang Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Building Blocks ◽  
Cell Uptake ◽  
Photon Absorption ◽  
In Vivo Studies ◽  
Infrared Light ◽  
Ros Generation ◽  
Multicellular Spheroids

Photodynamic therapy (PDT) is a treatment procedure that relies on cytotoxic reactive oxygen species (ROS) generated by the light activation of a photosensitizer. The photophysical and biological properties of photosensitizers are vital for the therapeutic outcome of PDT. In this work a 2D rhomboidal metallacycle and a 3D octahedral metallacage were designed and synthesized via the coordination-driven self-assembly of a Ru(II)-based photosensitizer and complementary Pt(II)-based building blocks. The metallacage showed deep-red luminescence, a large 2-photon absorption cross-section, and highly efficient ROS generation. The metallacage was encapsulated into an amphiphilic block copolymer to form nanoparticles to encourage cell uptake and localization. Upon internalization into cells, the nanoparticles selectively accumulate in the lysosomes, a favorable location for PDT. The nanoparticles are almost nontoxic in the dark, and can efficiently destroy tumor cells via the generation of ROS in the lysosomes under 2-photon near-infrared light irradiation. The superb PDT efficacy of the metallacage-containing nanoparticles was further validated by studies on 3D multicellular spheroids (MCS) and in vivo studies on A549 tumor-bearing mice.

Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy

2020 ◽  
Vol 63 (17) ◽  
pp. 9950-9964 ◽  
Author(s):  
Zhiliang Yu ◽  
Junliang Zhou ◽  
Xin Ji ◽  
Guangyu Lin ◽  
Shuang Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
In Vivo Evaluation

scholarly journals Rational design of a NIR-II fluorescent nano-system with maximized fluorescence performance and applications

2021 ◽  
Author(s):  
Haoli Yu ◽  
Yuesong Wang ◽  
Yan Chen ◽  
Min Ji
Keyword(s):  
Fluorescence Imaging ◽  
In Vivo Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Nir Fluorescence

Purpose: Near-infrared (NIR) fluorescence imaging (FI) become a research hotspot in the field of in vivo imaging. Here, we intend to synthesize a NIR-II fluorescence nano-system with an excellent fluorescence...

Photostable Iridium(III)–Cyanine Complex Nanoparticles for Photoacoustic Imaging Guided Near-Infrared Photodynamic Therapy in Vivo

2019 ◽  
Vol 11 (17) ◽  
pp. 15417-15425 ◽  
Author(s):  
Qi Yang ◽  
Hongyu Jin ◽  
Yucong Gao ◽  
Jiaomin Lin ◽  
Hong Yang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Photoacoustic Imaging

Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo

2012 ◽  
Vol 52 (4) ◽  
pp. 1175-1179 ◽  
Author(s):  
Ryosuke Kojima ◽  
Hideo Takakura ◽  
Takeaki Ozawa ◽  
Yukio Tada ◽  
Tetsuo Nagano ◽  
...  
Keyword(s):  
Rational Design ◽  
Near Infrared ◽  
Design And Development
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