Highly efficient BODIPY-doped upconversion nanoparticles for deep-red luminescence bioimaging in vivo

2021 ◽  
Author(s):  
Ti Jia ◽  
Qiuhong Wang ◽  
Ming Xu ◽  
Wei Yuan ◽  
Wei Feng ◽  
...  
Keyword(s):  
High Efficiency ◽  
Upconversion Luminescence ◽  
Excitation Power ◽  
Water Soluble ◽  
Upconversion Nanoparticles ◽  
Highly Efficient ◽  
Red Luminescence

We demonstrate a 3,5-di(p-oxethyl)styryl conjugated BODIPY showing deep-red upconversion luminescence with a high efficiency of 16.6%. Furthermore, water-soluble BODIPY-doped upconversion nanoparticles with efficiency up to 6.9% under low excitation power...

scholarly journals Highly efficient RNA-synthesizing system that uses isolated human mitochondria: new initiation events and in vivo-like processing patterns.

1984 ◽  
Vol 4 (8) ◽  
pp. 1605-1617 ◽  
Author(s):  
G Gaines ◽  
G Attardi
Keyword(s):  
Transcription Initiation ◽  
Ribosomal Proteins ◽  
High Efficiency ◽  
Rrna Synthesis ◽  
Isolated Mitochondria ◽  
Highly Efficient ◽  
Light Strand

A highly efficient RNA-synthesizing system with isolated HeLa cell mitochondria has been developed and characterized regarding its requirements and its products. In this system, transcription is initiated and the transcripts are processed in a way which closely reproduces the in vivo patterns. Total RNA labeling in isolated mitochondria proceeds at a constant rate for about 30 min at 37 degrees C; the estimated rate of synthesis is at least 10 to 15% of the in vivo rate. Polyadenylation of the mRNAs is less extensive in this system than in vivo. Furthermore, compared with the in vivo situation, rRNA synthesis in vitro is less efficient than mRNA synthesis. This is apparently due to a decreased rate of transcription initiation at the rRNA promoter and probably a tendency also for premature termination of the nascent rRNA chains. The 5'-end processing of rRNA also appears to be slowed down, and it is very sensitive to the incubation conditions, in contrast to mRNA processing. It is suggested that the lower efficiency and the lability of rRNA synthesis and processing in isolated mitochondria may be due to cessation of import from the cytoplasm of ribosomal proteins that play a crucial role in these processes. The formation of the light-strand-coded RNA 18 (7S RNA) is affected by high pH or high ATP concentration differently from the overall light-strand transcription. The dissociation of the two processes may have important implications for the mechanism of formation and the functional role of this unusual RNA species. The high efficiency, initiation capacity, and processing fidelity of the in vitro RNA-synthesizing system described here make it a valuable tool for the analysis of the role of nucleocytoplasmic-mitochondrial interactions in organelle gene expression.

Upconversion-luminescent/magnetic dual-functional sub-20 nm core–shell SrF2:Yb,Tm@CaF2:Gd heteronanoparticles

2016 ◽  
Vol 45 (13) ◽  
pp. 5800-5807 ◽  
Author(s):  
Ai-Hua Li ◽  
Mengyun Lü ◽  
Jun Yang ◽  
Lin Chen ◽  
Xiaohong Cui ◽  
...  
Keyword(s):  
High Efficiency ◽  
Upconversion Luminescence ◽  
Water Soluble ◽  
Core Shell ◽  
Dual Functional ◽  
Magnetic Shell ◽  
20 Nm

Combination of a high-efficiency upconversion luminescence core with a biocompatible magnetic shell endows hydrothermal-synthesized water-soluble nanoparticles with improved dual-modal bioimaging capabilities.

scholarly journals Ultra-stable near-infrared Tm3+-doped upconversion nanoparticles for in vivo wide-field two-photon angiography with a low excitation intensity

2019 ◽  
Vol 12 (03) ◽  
pp. 1950013 ◽  
Author(s):  
Wen Liu ◽  
Runze Chen ◽  
Sailing He
Keyword(s):  
Power Density ◽  
Near Infrared ◽  
Multilayer Coating ◽  
Excitation Power ◽  
Whole Body ◽  
Upconversion Nanoparticles ◽  
Excitation Power Density ◽  
Two Photon ◽  
Coating Method

Two-photon luminescence with near-infrared (NIR) excitation of upconversion nanoparticles (NPs) is of great importance in biological imaging due to deep penetration in high-scattering tissues, low auto-luminescence and good sectioning ability. Unfortunately, common two-photon luminescence is in visible band with an extremely high exciation power density, which limits its application. Here, we synthesized NaYF4:Yb[Formula: see text]Tm@NaYF4 upconversion NPs with strong two-photon NIR emission and a low excitation power density. Furthermore, NaYF4:Yb[Formula: see text]Tm@NaYF4@SiO2@OTMS@F127 NPs with high chemical stability were obtained by a modified multilayer coating method which was applied to upconversion NPs for the first time. In addition, it is shown that the as-prepared hydrophillic upconversion NPs have great biocompatibility and kept stable for 6 hours during in vivo whole-body imaging. The vessels with two-photon luminescence were clear even under an excitation power density as low as 25[Formula: see text]mW[Formula: see text]cm2. Vivid visualizations of capillaries and vessels in a mouse brain were also obtained with low background and high contrast. Because of cheaper instruments and safer power density, the NIR two-photon luminescence of NaYF4:Yb[Formula: see text]Tm@NaYF4 upconversion NPs could promote wider application of two-photon technology. The modified multilayer coating method could be widely used for upconversion NPs to increase the stable time of the in vivo circulation. Our work possesses a great potential for deep imaging and imaging-guided treatment in the future.

scholarly journals Highly Doped Upconversion Nanoparticles for In Vivo Applications Under Mild Excitation Power

2020 ◽  
Vol 92 (16) ◽  
pp. 10913-10919
Author(s):  
Du Li ◽  
Shihui Wen ◽  
Mengya Kong ◽  
Yongtao Liu ◽  
Wei Hu ◽  
...  
Keyword(s):  
Excitation Power ◽  
Upconversion Nanoparticles

High-Efficiency in Vitro and in Vivo Detection of Zn2+ by Dye-Assembled Upconversion Nanoparticles

2015 ◽  
Vol 137 (6) ◽  
pp. 2336-2342 ◽  
Author(s):  
Juanjuan Peng ◽  
Wang Xu ◽  
Chai Lean Teoh ◽  
Sanyang Han ◽  
Beomsue Kim ◽  
...  
Keyword(s):  
High Efficiency ◽  
Upconversion Nanoparticles ◽  
In Vivo Detection

scholarly journals Chloroplast thioredoxin mutants without active-site cysteines facilitate the reduction of the regulatory disulphide bridge on the γ-subunit of chloroplast ATP synthase

1999 ◽  
Vol 341 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Michael T. STUMPP ◽  
Ken MOTOHASHI ◽  
Toru HISABORI
Keyword(s):  
Active Site ◽  
High Efficiency ◽  
Expression System ◽  
Water Soluble ◽  
Subsequent Reduction ◽  
Disulphide Bridge ◽  
Mutant Proteins ◽  
Apparent Affinity ◽  
Γ Subunit

The activity of the chloroplast H+-ATPase (CFoCF1) is regulated by the proton electrochemical membrane potential and the reduction or the formation of the disulphide bridge on the γ-subunit mediated by chloroplast thioredoxins (Trx). The latter regulation also applies to the water-soluble portion of CFoCF1 (CF1) and includes two successive steps, namely the binding of Trx to CF1 and the subsequent reduction or oxidation of CF1. To study this process thoroughly, a new expression system for spinach Trx-f and Trx-m was designed. In the presence of dithiothreitol (DTT) both forms of the expressed Trx could reduce the disulphide bridge on the γ-subunit of CF1 and thus activate the ATPase. Trx mutants deficient in the internal, or both, cysteines of the active site were designed to study the details of the interaction. The Trx mutant proteins could still activate CF1-ATPase in the presence of DTT and they also increased the apparent affinity of CF1 for DTT. This implies that the binding of Trx to the CF1 γ-subunit induces a conformational change facilitating the reduction of the disulphide bridge, and partially explains the high efficiency of Trx as a reductant in vivo.

scholarly journals Long-Term Tri-Modal In Vivo Tracking of Engrafted Cartilage-Derived Stem/Progenitor Cells Based on Upconversion Nanoparticles

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 958
Author(s):  
Chu-Hsin Chen ◽  
Na Tang ◽  
Ke Xue ◽  
Hui-Zhong Zhang ◽  
Ya-Hong Chen ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Upconversion Luminescence ◽  
Operating Costs ◽  
Upconversion Nanoparticles ◽  
Cell Numbers ◽  
Detectable Range ◽  
X Ray Computed ◽  
Tracing Techniques

Cartilage-derived stem/progenitor cells (CSPCs) are a potential choice for seed cells in osteal and chondral regeneration, and the outcomes of their survival and position distribution in vivo form the basis for the investigation of their mechanism. However, the current use of in vivo stem cell tracing techniques in laboratories is relatively limited, owing to their high operating costs and cytotoxicity. Herein, we performed tri-modal in vivo imaging of CSPCs during subcutaneous chondrogenesis using upconversion nanoparticles (UCNPs) for 28 days. Distinctive signals at accurate positions were acquired without signal noise from X-ray computed tomography, magnetic resonance imaging, and upconversion luminescence. The measured intensities were all significantly proportional to the cell numbers, thereby enabling real-time in vivo quantification of the implanted cells. However, limitations of the detectable range of cell numbers were also observed, owing to the imaging shortcomings of UCNPs, which requires further improvement of the nanoparticles. Our study explores the application value of upconversion nanomaterials in the tri-modal monitoring of implanted stem cells and provides new perspectives for future clinical translation.

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