Organic fluorescent probes for monitoring autophagy in living cells

2021 ◽  
Author(s):  
Xuechen Li ◽  
Xing Liang ◽  
Junling Yin ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probes ◽  
Recent Progress ◽  
Living Cells ◽  
First Time

The recent progress in the development of organic fluorescent probes for monitoring the autophagy process in living cells has been reviewed for the first time.

scholarly journals Enhancing biocompatibility of rhodamine fluorescent probes by a neighbouring group effect

2020 ◽  
Author(s):  
Jonas Bucevičius ◽  
Georgij Kostiuk ◽  
Rūta Gerasimaitė ◽  
Tanja Gilat ◽  
Gražvydas Lukinavičius
Keyword(s):  
Fluorescent Probes ◽  
Staining Intensity ◽  
Living Cells ◽  
Amide Group ◽  
Cell Permeability ◽  
Cellular Structures ◽  
High Contrast ◽  
Group Effect ◽  
First Time

AbstractFluorescence microscopy is an essential tool for understanding dynamic processes in living cells and organisms. However, many fluorescent probes for labelling cellular structures suffer from unspecific interactions and low cell permeability. Herein, we demonstrate that the neighbouring group effect which results from positioning an amide group next to a carboxyl group in the benzene ring of rhodamines dramatically increases cell permeability of the rhodamine-based probes through stabilizing a fluorophore in a hydrophobic spirolactone state. Based on this principle, we create probes targeting tubulin, actin and DNA. Their superb staining intensity, tuned toxicity and specificity allows long-term 3D confocal and STED nanoscopy with sub-30 nm resolution. As a result, the real microtubule diameter of 23 nm was resolved inside a living cell for the first time. Due to their unrestricted cell permeability and efficient accumulation on tubulin, the new probes produce high contrast images at sub-nanomolar concentrations.

Recent progress on the organic and metal complex-based fluorescent probes for monitoring nitric oxide in living biological systems

2020 ◽  
Vol 18 (8) ◽  
pp. 1522-1549 ◽  
Author(s):  
Lizhen Wang ◽  
Juan Zhang ◽  
Xue An ◽  
Hongdong Duan
Keyword(s):  
Nitric Oxide ◽  
Metal Complex ◽  
Fluorescent Probes ◽  
Recent Progress ◽  
Biological Systems ◽  
Living Cells ◽  
Significant Progress ◽  
The Past

This article reviews the significant progress of novel fluorescent probes used for imaging NO in living cells and animals during the past five years.

scholarly journals Development of High-Specificity Fluorescent Probes to Enable Cannabinoid Type 2 Receptor Studies in Living Cells

2020 ◽  
Author(s):  
Roman Sarott ◽  
Matthias Westphal ◽  
Patrick Pfaff ◽  
Claudia Korn ◽  
David Sykes ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Modular Design ◽  
Inflammatory Diseases ◽  
High Specificity ◽  
Cell Types ◽  
Living Cells ◽  
Binding Assays ◽  
Mouse Splenocytes ◽  
First Time

Pharmacological modulation of cannabinoid type 2 receptor (CB<sub>2</sub>R) holds promise for the treatment of numerous conditions, including inflammatory diseases, autoimmune disorders, pain, and cancer. Despite the significance of this receptor, researchers lack reliable tools to address questions concerning the expression and complex mechanism of CB<sub>2</sub>R signaling, especially in cell-type and tissue-dependent context. Herein, we report for the first time a versatile ligand platform for the modular design of a collection of highly specific CB<sub>2</sub>R fluorescent probes, used successfully across applications, species and cell types. These include flow cytometry of endogenously expressing cells, real-time confocal microscopy of mouse splenocytes and human macrophages, as well as FRET-based kinetic and equilibrium binding assays. High CB<sub>2</sub>R specificity was demonstrated by competition experiments in living cells expressing CB<sub>2</sub>R at native levels. The probes were effectively applied to FACS analysis of microglial cells derived from a mouse model relevant to Alzheimer’s disease and to the detection of CB<sub>2</sub>R in human breast cancer cells.

scholarly journals Development of High-Specificity Fluorescent Probes to Enable Cannabinoid Type 2 Receptor Studies in Living Cells

2020 ◽  
Author(s):  
Roman Sarott ◽  
Matthias Westphal ◽  
Patrick Pfaff ◽  
Claudia Korn ◽  
David Sykes ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Modular Design ◽  
Inflammatory Diseases ◽  
High Specificity ◽  
Cell Types ◽  
Living Cells ◽  
Binding Assays ◽  
Mouse Splenocytes ◽  
First Time

Pharmacological modulation of cannabinoid type 2 receptor (CB<sub>2</sub>R) holds promise for the treatment of numerous conditions, including inflammatory diseases, autoimmune disorders, pain, and cancer. Despite the significance of this receptor, researchers lack reliable tools to address questions concerning the expression and complex mechanism of CB<sub>2</sub>R signaling, especially in cell-type and tissue-dependent context. Herein, we report for the first time a versatile ligand platform for the modular design of a collection of highly specific CB<sub>2</sub>R fluorescent probes, used successfully across applications, species and cell types. These include flow cytometry of endogenously expressing cells, real-time confocal microscopy of mouse splenocytes and human macrophages, as well as FRET-based kinetic and equilibrium binding assays. High CB<sub>2</sub>R specificity was demonstrated by competition experiments in living cells expressing CB<sub>2</sub>R at native levels. The probes were effectively applied to FACS analysis of microglial cells derived from a mouse model relevant to Alzheimer’s disease and to the detection of CB<sub>2</sub>R in human breast cancer cells.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

scholarly journals Structure modulation on fluorescent probes for biothiols and the reversible imaging of glutathione in living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 21116-21126
Author(s):  
Yu Li ◽  
Li Chen ◽  
Yan Zhu ◽  
Liming Chen ◽  
Xianglin Yu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Structure Modulation

A reversible fluorescent probe for GSH was obtained through structure modulation, by which the intracellular GSH fluctuation was imaged.

Recent advances in 1,8-naphthalimide-based small-molecule fluorescent probes for organelles imaging and tracking in living cells

2021 ◽  
Vol 444 ◽  
pp. 214019
Author(s):  
Haitao Yu ◽  
Yan Guo ◽  
Wencheng Zhu ◽  
Kaden Havener ◽  
Xujun Zheng
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Recent Advances

Reasonably constructed NIR fluorescent probes based on dicyanoisophorone skeleton for imaging ONOO− in living cells

2021 ◽  
pp. 109665
Author(s):  
Jeewon Chung ◽  
Heejeong Kim ◽  
Haidong Li ◽  
Juyoung Yoon
Keyword(s):  
Fluorescent Probes ◽  
Living Cells
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