scholarly journals Sensitive and enzyme-free fluorescence polarization detection for miRNA-21 based on decahedral sliver nanoparticles and strand displacement reaction

RSC Advances ◽  
2020 ◽  
Vol 10 (29) ◽  
pp. 17037-17044
Author(s):  
Qingyue Zhu ◽  
Hui Li ◽  
Danke Xu
Keyword(s):  
Fluorescence Polarization ◽  
Signal Amplification ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Polarization Detection ◽  
Sliver Nanoparticles ◽  
Sensitive Method ◽  
Strand Displacement Reaction

A highly sensitive method for miRNA-21 detection has been developed, which relied on the principle of strand displacement reaction to achieve asymmetric signal amplification and combined with the enhanced effect of Ag10NPs.

scholarly journals A kinetically controlled platform for ligand-oligonucleotide transduction

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Qiu-Long Zhang ◽  
Liang-Liang Wang ◽  
Yan Liu ◽  
Jiao Lin ◽  
Liang Xu
Keyword(s):  
Nucleic Acid ◽  
Small Molecules ◽  
Signal Amplification ◽  
Kinetic Control ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Critical Pathway ◽  
Kinetically Controlled ◽  
Binding Sequence ◽  
Strand Displacement Reaction

AbstractLigand-oligonucleotide transduction provides the critical pathway to integrate non-nucleic acid molecules into nucleic acid circuits and nanomachines for a variety of strand-displacement related applications. Herein, a general platform is constructed to convert the signals of ligands into desired oligonucleotides through a precise kinetic control. In this design, the ligand-aptamer binding sequence with an engineered duplex stem is introduced between the toehold and displacement domains of the invading strand to regulate the strand-displacement reaction. Employing this platform, we achieve efficient transduction of both small molecules and proteins orthogonally, and more importantly, establish logical and cascading operations between different ligands for versatile transduction. Besides, this platform is capable of being directly coupled with the signal amplification systems to further enhance the transduction performance. This kinetically controlled platform presents unique features with designing simplicity and flexibility, expandable complexity and system compatibility, which may pave a broad road towards nucleic acid-based developments of sophisticated transduction networks.

A homogeneous fluorescent biosensing strategy for highly sensitive detection of DNA based on a programmed entropy-driven strand displacement reaction and DNAzyme for dual recycling amplification

2019 ◽  
Vol 11 (12) ◽  
pp. 1613-1619 ◽  
Author(s):  
Yujian Li ◽  
Xiaojuan Ding ◽  
Dandan Li ◽  
Haiping Wu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Fluorescent Biosensing ◽  
Recycling Amplification ◽  
Highly Sensitive Detection ◽  
Strand Displacement Reaction

In this study, a homogeneous fluorescent biosensing strategy is described for the detection of DNA based on an entropy-driven strand displacement reaction and DNAzyme.

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