Label-free and sensitive detection of RNA demethylase FTO with primer generation rolling circle amplification

2022 ◽  
Author(s):  
Xiaoxia Han ◽  
Yue-Ying Li ◽  
Zi-Yue Wang ◽  
Ling-Zhi Liu ◽  
Bing-Jie Liu ◽  
...  
Keyword(s):  
Fat Mass ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Method ◽  
First Time

We develop for the first time a label-free fluorescent method for sensitive detection of fat mass and obesity-associated protein (FTO) activity using MazF-mediated primer generation rolling circle amplification. This method...

Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

2021 ◽  
Vol 57 (16) ◽  
pp. 2041-2044
Author(s):  
Su Jiang ◽  
Meng Liu ◽  
Wei Tantai ◽  
Qinfeng Xu ◽  
Xiaoran Zou ◽  
...  
Keyword(s):  
Histone Modification ◽  
Histone Acetyltransferase ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Site Specific ◽  
Rolling Circle ◽  
Acetyltransferase Activity ◽  
Label Free Detection ◽  
Histone Acetyltransferase Activity

An aptamer-mediated histone modification site-specific rolling circle amplification is developed for label-free detection of histone-modifying enzyme at the femtomolar level.

Label-free and sensitive detection of uracil-DNA glycosylase using exponential real-time rolling circle amplification

2018 ◽  
Vol 10 (20) ◽  
pp. 2405-2410 ◽  
Author(s):  
Yuan Xu ◽  
Yun-Xi Cui ◽  
Qiu-Ge Zhao ◽  
An-Na Tang ◽  
De-Ming Kong
Keyword(s):  
Real Time ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Schematic Representation ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Activity Sensing

Schematic representation of an exponential rolling circle amplification-based uracil-DNA glycosylase activity-sensing platform.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

Sensitive and label-free discrimination of 5-hydroxymethylcytosine and 5-methylcytosine in DNA by ligation-mediated rolling circle amplification

2018 ◽  
Vol 54 (62) ◽  
pp. 8602-8605 ◽  
Author(s):  
Zi-yue Wang ◽  
Meng Wang ◽  
Yan Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Rolling Circle Amplification ◽  
Fluorescence Method ◽  
Label Free ◽  
Rolling Circle

We develop a label-free fluorescence method for sensitive discrimination of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) by ligation-mediated rolling circle amplification.

Sensitive Osteosarcoma Diagnosis Through Five-Base Telomerase Product-Triggered CRISPR-Cas12a Enhanced Rolling Circle Amplification

2021 ◽  
Author(s):  
Xing Zhou ◽  
Jun-Liang Zhang ◽  
Meng-Han Chang ◽  
Gentao Fan ◽  
Xiao-Zhou Liu ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Bone Tumor ◽  
Rolling Circle Amplification ◽  
Mesenchymal Cells ◽  
Pivotal Role ◽  
Sensitive Detection ◽  
Malignant Bone Tumor ◽  
Rolling Circle ◽  
Primary Malignant Bone Tumor

Osteosarcoma is the most frequent primary malignant bone tumor composed of mesenchymal cells producing osteoid and immature bone. Sensitive detection of telomerase plays a pivotal role for the early diagnosis...

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