Biotransformations and cytotoxicity of graphene and inorganic two-dimensional nanomaterials using simulated digestions coupled with a triculture in vitro model of the human gastrointestinal epithelium

Schizophrenia is a multifactorial psychiatric disorder, which affects about 1% of the world's population. Several studies have demonstrated the involvement of the endocannabinoid system in the pathophysiology of schizophrenia. Studies have shown that endocannabinoids can also affect the function of oligodendrocytes. In order to better understand the mechanisms involved, and to help developing less harmful treatments, we will investigate the effects of endocannabinoids and synthetic cannabinoid agonists on a human oligodendrocyte cell line (MO3.13). In addition, we will verify if these treatments may reverse the effects of the NMDAr antagonist MK801 - an in vitro model to study schizophrenia. For this, we will use the two-dimensional liquid nanocromatography coupled to high resolution mass spectrometry. Data will be processed and analysed using in silico system biology tools. Taken together, our approach aims to contribute to the elucidation of the role of activation of cannabinoid receptorsin oligodendrocytes and the possible implications for undertanding the pathophysiology of schizophrenia.

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An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2005.06.014 ◽

2006 ◽

Vol 150 (2) ◽

pp. 192-201 ◽

Cited By ~ 124

Author(s):

Barclay Morrison ◽

Heather L. Cater ◽

Christopher D. Benham ◽

Lars E. Sundstrom

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

In Vitro Model ◽

Hippocampal Slice ◽

Two Dimensional ◽

Hippocampal Slice Cultures ◽

Vitro Model ◽

Organotypic Hippocampal Slice Cultures

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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