Prebiotic activities of dextran from Leuconostoc mesenteroides SPCL742 analyzed in the aspect of the human gut microbial ecosystem

2022 ◽  
Author(s):  
Geonhee Kim ◽  
Jae-Han Bae ◽  
Seongwon Cheon ◽  
Dong Hyeon Lee ◽  
Da Hye Kim ◽  
...  
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Microbial Ecosystem

Investigation of the prebiotic activities of LM742 dextran produced by Leuconostoc mesenteroides SPCL742 in the aspect of the human gut microbial ecosystem focusing on microbiome and metabolome changes in in vitro colonic fermentation.

scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

Challenges in simulating the human gut for understanding the role of the microbiota in obesity

2017 ◽  
Vol 8 (1) ◽  
pp. 31-53 ◽  
Author(s):  
M. Aguirre ◽  
K. Venema
Keyword(s):  
Potential Role ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Physiological Relevance ◽  
In Vitro Simulation ◽  
In Vitro Systems ◽  
Specific Standard ◽  
Obese Subjects

There is an elevated incidence of cases of obesity worldwide. Therefore, the development of strategies to tackle this condition is of vital importance. This review focuses on the necessity of optimising in vitro systems to model human colonic fermentation in obese subjects. This may allow to increase the resolution and the physiological relevance of the information obtained from this type of studies when evaluating the potential role that the human gut microbiota plays in obesity. In light of the parameters that are currently used for the in vitro simulation of the human gut (which are mostly based on information derived from healthy subjects) and the possible difference with an obese condition, we propose to revise and improve specific standard operating procedures.

In Vitro Colonic Fermentation of Saponin-Rich Extracts from Quinoa, Lentil, and Fenugreek. Effect on Sapogenins Yield and Human Gut Microbiota

2019 ◽  
Vol 68 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Joaquín Navarro del Hierro ◽  
Carolina Cueva ◽  
Alba Tamargo ◽  
Estefanía Núñez-Gómez ◽  
M. Victoria Moreno-Arribas ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiota

scholarly journals Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions

2020 ◽  
Vol 8 (10) ◽  
pp. 1582
Author(s):  
Shanthi Parkar ◽  
Carel Jobsis ◽  
Tania Trower ◽  
Janine Cooney ◽  
Duncan Hedderley ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Gene Expression Analysis ◽  
Serotonin Synthesis ◽  
Gastrointestinal Digestion ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro System ◽  
Physiological Relevance ◽  
Intestinal Functions

Whole kiwifruit (‘Hayward’ and ‘Zesy002’) were examined for their bioaminergic potential after being subjected to in vitro gastrointestinal digestion and colonic fermentation. Controls included the prebiotic inulin and water, a carbohydrate-free vehicle. The dopamine precursor l-dihydroxyphenylalanine (L-DOPA) and the serotonin precursor 5-hydroxytryptophan were increased in the kiwifruit gastrointestinal digesta (‘Hayward’ > ‘Zesy002’) in comparison to the water digesta. Fermentation of the digesta with human fecal bacteria for 18 h modulated the concentrations of bioamine metabolites. The most notable were the significant increases in L-DOPA (‘Zesy002’ > ‘Hayward’) and γ-aminobutyric acid (GABA) (‘Hayward’ > ‘Zesy002’). Kiwifruit increased Bifidobacterium spp. and Veillonellaceae (correlating with L-DOPA increase), and Lachnospira spp. (correlating with GABA). The digesta and fermenta were incubated with Caco-2 cells for 3 h followed by gene expression analysis. Effects were seen on genes related to serotonin synthesis/re-uptake/conversion to melatonin, gut tight junction, inflammation and circadian rhythm with different digesta and fermenta from the four treatments. These indicate potential effects of the substrates and the microbially generated organic acid and bioamine metabolites on intestinal functions that have physiological relevance. Further studies are required to confirm the potential bioaminergic effects of gut microbiota–kiwifruit interactions.

Plant sterols and human gut microbiota relationship: An in vitro colonic fermentation study

2018 ◽  
Vol 44 ◽  
pp. 322-329 ◽  
Author(s):  
María Cuevas-Tena ◽  
Eva María Gómez del Pulgar ◽  
Alfonso Benítez-Páez ◽  
Yolanda Sanz ◽  
Amparo Alegría ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Plant Sterols ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiota

scholarly journals A decrease in iron availability to human gut microbiome reduces the growth of potentially pathogenic gut bacteria; an in vitro colonic fermentation study

2019 ◽  
Vol 67 ◽  
pp. 20-27 ◽  
Author(s):  
Bhavika A Parmanand ◽  
Lee Kellingray ◽  
Gwenaelle Le Gall ◽  
Abdul W Basit ◽  
Susan Fairweather-Tait ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Gut Bacteria ◽  
Iron Availability ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiome

scholarly journals In vitro colonic fermentation of Mexican “taco” from corn-tortilla and black beans in a Simulator of Human Microbial Ecosystem (SHIME®) system

2019 ◽  
Vol 118 ◽  
pp. 81-88 ◽  
Author(s):  
Alicia Paulina Cárdenas-Castro ◽  
Fernanda Bianchi ◽  
María Angela Tallarico-Adorno ◽  
Efigenia Montalvo-González ◽  
Sonia G. Sáyago-Ayerdi ◽  
...  
Keyword(s):  
Colonic Fermentation ◽  
Microbial Ecosystem ◽  
Black Beans ◽  
Corn Tortilla

In vitro study of the interaction between human gut microbiota and herbal extracts using willow bark extract as an example

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer
Keyword(s):  
Gut Microbiota ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bark Extract ◽  
Herbal Extracts ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Willow Bark
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