scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

2021 ◽  
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Hong Yao ◽  
Barbara Williams ◽  
Bernadine Flanagan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Cell Walls ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Plant Cell Walls ◽  
Human Gut ◽  
In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

Effects of exopolysaccharide from Lactobacillus rhamnosus on human gut microbiota in in vitro fermentation model

LWT ◽  
2020 ◽  
pp. 110524
Author(s):  
Yuzhu Zhu ◽  
Jia-Min Zhou ◽  
Wei Liu ◽  
Xionge Pi ◽  
Qingqing Zhou ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Lactobacillus Rhamnosus ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota ◽  
Fermentation Model

scholarly journals In Vitro Fermentation Patterns of Rice Bran Components by Human Gut Microbiota

Nutrients ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 1237 ◽  
Author(s):  
Tung Pham ◽  
Keat Teoh ◽  
Brett Savary ◽  
Ming-Hsuan Chen ◽  
Anna McClung ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Rice Bran ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota

In vitro fermentation of alginate and its derivatives by human gut microbiota

Anaerobe ◽  
2016 ◽  
Vol 39 ◽  
pp. 19-25 ◽  
Author(s):  
Miaomiao Li ◽  
Guangsheng Li ◽  
Qingsen Shang ◽  
Xiuxia Chen ◽  
Wei Liu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota

In Vitro Colonic Fermentation of Saponin-Rich Extracts from Quinoa, Lentil, and Fenugreek. Effect on Sapogenins Yield and Human Gut Microbiota

2019 ◽  
Vol 68 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Joaquín Navarro del Hierro ◽  
Carolina Cueva ◽  
Alba Tamargo ◽  
Estefanía Núñez-Gómez ◽  
M. Victoria Moreno-Arribas ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiota

In vitro fermentation of κ-carrageenan oligosaccharides by human gut microbiota and its inflammatory effect on HT29 cells

2019 ◽  
Vol 59 ◽  
pp. 80-91 ◽  
Author(s):  
Yujiao Sun ◽  
Xiangyi Cui ◽  
Mengmeng Duan ◽  
Chunqing Ai ◽  
Shuang Song ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota ◽  
Ht29 Cells ◽  
Inflammatory Effect

scholarly journals Structure of β-glucan from Tibetan hull-less barley and its in vitro fermentation by human gut microbiota

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Chenxi Nie ◽  
Xin Yan ◽  
Xiaoqing Xie ◽  
Ziqi Zhang ◽  
Jiang Zhu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Ph Value ◽  
Food Supplement ◽  
Water Soluble ◽  
Spherical Particles ◽  
Human Gut ◽  
Sem Images ◽  
In Vitro Fermentation ◽  
Human Gut Microbiota

Abstract Background The influence of β-glucan on the human gut microbiota is closely related to the physicochemical structure of β-glucan. We purified a homogeneous water-soluble polysaccharide from Tibetan hull-less barley 25 and studied its structure and the in vitro fermentation profile. Results Analysis by gas chromatography (GC), Fourier-transformed infrared (FT-IR), nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), and atomic force micrograph (AFM) helped determine the hull-less barley polysaccharide to be a β-glucan (molecular weight: 3.45 × 104 Da), which was further characterized as mixed-linkage (1 → 3)(1 → 4)-linked β-d-glucans. SEM images demonstrated an intricate web structure of the hull-less barley polysaccharide, while the AFM images revealed the presence of small spherical particles in its structure. In addition, the microbiota composition of the hull-less barley polysaccharide group was found to be altered, wherein the abundance of Pantoea, Megamonas, Bifidobacterium, and Prevotella-9 were increased. On the other hand, in vitro fermentation revealed that hull-less barley polysaccharide significantly decreased the pH value and increased the production of acetate, propionate, and butyrate. Conclusions Hull-less barley polysaccharide is a type of dietary fiber, and its analysis suggested that it may serve as a prebiotic food supplement for the regulation of the gut microbiota.

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