In Vitro Colonic Fermentation of Saponin-Rich Extracts from Quinoa, Lentil, and Fenugreek. Effect on Sapogenins Yield and Human Gut Microbiota

2019 ◽  
Vol 68 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Joaquín Navarro del Hierro ◽  
Carolina Cueva ◽  
Alba Tamargo ◽  
Estefanía Núñez-Gómez ◽  
M. Victoria Moreno-Arribas ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiota

Plant sterols and human gut microbiota relationship: An in vitro colonic fermentation study

2018 ◽  
Vol 44 ◽  
pp. 322-329 ◽  
Author(s):  
María Cuevas-Tena ◽  
Eva María Gómez del Pulgar ◽  
Alfonso Benítez-Páez ◽  
Yolanda Sanz ◽  
Amparo Alegría ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Plant Sterols ◽  
Human Gut ◽  
Colonic Fermentation ◽  
Human Gut Microbiota

In vitro study of the interaction between human gut microbiota and herbal extracts using willow bark extract as an example

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer
Keyword(s):  
Gut Microbiota ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bark Extract ◽  
Herbal Extracts ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Willow Bark

Metabolization of the herbal combination STW-5 by human gut microbiota in vitro

2017 ◽  
Author(s):  
EM Pferschy-Wenzig ◽  
A Roßmann ◽  
K Koskinen ◽  
H Abdel-Aziz ◽  
C Moissl-Eichinger ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Human Gut Microbiota

scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

Analysis of in vitro digestion using human gut microbiota in adult and elderly individuals

2021 ◽  
pp. 130228
Author(s):  
Seung Yun Lee ◽  
Da Young Lee ◽  
Hea Jin Kang ◽  
Ji Hyeop Kang ◽  
Hae Won Jang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
In Vitro Digestion ◽  
Human Gut ◽  
Elderly Individuals ◽  
Human Gut Microbiota

In Vitro Effects of Stachyose on the Human Gut Microbiota

2021 ◽  
pp. 2100029
Author(s):  
Zhonglin Zhao ◽  
Wei Liu ◽  
Xionge Pi
Keyword(s):  
Gut Microbiota ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
In Vitro Effects

scholarly journals Effects of phlorotannin and polysaccharide fractions of brown seaweed Silvetia compressa on human gut microbiota composition using an in vitro colonic model

2021 ◽  
Vol 84 ◽  
pp. 104596
Author(s):  
Benjamín Vázquez-Rodríguez ◽  
Liliana Santos-Zea ◽  
Erick Heredia-Olea ◽  
Laura Acevedo-Pacheco ◽  
Arlette Santacruz ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Brown Seaweed ◽  
Microbiota Composition ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Gut Microbiota Composition

A pectic polysaccharide from peach palm fruits (Bactris gasipaes) and its fermentation profile by the human gut microbiota in vitro

2017 ◽  
Vol 9 ◽  
pp. 1-6 ◽  
Author(s):  
Thaisa Moro Cantu-Jungles ◽  
Thales R. Cipriani ◽  
Marcello Iacomini ◽  
Bruce R. Hamaker ◽  
Lucimara M.C. Cordeiro
Keyword(s):  
Gut Microbiota ◽  
Bactris Gasipaes ◽  
Human Gut ◽  
Pectic Polysaccharide ◽  
Peach Palm ◽  
Human Gut Microbiota ◽  
Fermentation Profile ◽  
Palm Fruits
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