Centrifugal microfluidic chip for point-of-care testing of staphylococcal enterotoxin B in complex matrixes

Nanoscale ◽  
2021 ◽  
Author(s):  
Li Li Tian ◽  
Chun Hong Li ◽  
Qi Chao Ye ◽  
Yuan Fang Li ◽  
Cheng Zhi Huang ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Detection Methods ◽  
Staphylococcal Enterotoxin B ◽  
Point Of Care Testing ◽  
Preparation Time ◽  
Enterotoxin B

Staphylococcal enterotoxin B (SEB) is a typical biological toxin that causes food poisoning. Currently reported SEB detection methods had the drawbacks of sophisticated sample preparation, time-consuming and labor-intensive. Herein, we...

scholarly journals Staphylococcal enterotoxin B (SEB) toxin of Staphylococcus aureus and the detection methods

2018 ◽  
Vol 15 (2) ◽  
pp. 211-221
Author(s):  
Nguyễn Thị Hoài Thu ◽  
Nghiêm Ngọc Minh
Keyword(s):  
Staphylococcus Aureus ◽  
Proteolytic Enzymes ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Water Soluble ◽  
Detection Methods ◽  
Staphylococcal Enterotoxin B ◽  
Heat Stable ◽  
Enterotoxin B ◽  
Basic Features

Staphylococcal enterotoxins (SEs) secreted by Staphylococcus aureus is one of the principal causes of food poisoning. The SEs are superantigens; they are highly stable, resisting most proteolytic enzymes and thus keeping activity in the gastrointestinal tract after being ingestion. In particular, heat-stable enterotoxin is one of the most important property related to food safety. They are not degraded at 100°C for 30 minutes, even at 121oC for 28 minutes, the SEs retain biological activity. Heat resistance of SEs in foods is higher than in the culture medium. Staphylococcus aureus (S. aureus) produces more than 20 different types of enterotoxins, including SEA to SEE, SEG to SER and SEU. Among these, Staphylococcal enterotoxin B (SEB) is a powerful toxin, heat-stable, water-soluble and is a common cause of food poisoning. Moreover, SEB is one of the harmful or hazardous agents used as biological weapons in bioterrorism or biological warfare. Therefore, determining presence of SEB toxin in food is extremely important. In this review, we introduce the most basic features about S. aureus; about SEB toxin and conventional methods for SEB diagnosis, detection. Especially, we focus on rapid detection strip based on an immunochromatography; this technique is an highly sensitive, rapid, easy for use and storage.

scholarly journals An automated point-of-care system for immunodetection of staphylococcal enterotoxin B

2011 ◽  
Vol 416 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Minghui Yang ◽  
Steven Sun ◽  
Yordan Kostov ◽  
Avraham Rasooly
Keyword(s):  
Point Of Care ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Point Of Care System ◽  
Enterotoxin B ◽  
Care System

scholarly journals Detection of Staphylococcal Enterotoxin B in Milk and Milk Products Using Immunodiagnostic Lateral Flow Devices

2010 ◽  
Vol 93 (2) ◽  
pp. 569-575 ◽  
Author(s):  
Thomas Boyle ◽  
Joyce M Njoroge ◽  
Robert L Jones ◽  
Maryann Principato
Keyword(s):  
Capillary Flow ◽  
Food Poisoning ◽  
Ice Cream ◽  
Extraction Methods ◽  
Etiologic Agent ◽  
Staphylococcal Enterotoxin ◽  
Lateral Flow ◽  
Staphylococcal Enterotoxin B ◽  
Hook Effect ◽  
Enterotoxin B

Abstract Staphylococcal enterotoxin B (SEB) is an extracellular pyrotoxin produced by Staphylococcus aureus, a known etiologic agent of food poisoning in humans. Lateral flow immunochromatographic devices (LFDs) designed for the environmental detection of SEB were adapted for use in this study to detect SEB in milk containing 2 fat, chocolate-flavored milk, and milk-derived products such as yogurt, infant formula, and ice cream. The advantage of using LFDs in these particular food products was its ease and speed of use with no additional extraction methods needed. No false positives were observed with any of the products used in this study. Dilution of the samples overcame the Hook effect and permitted capillary flow into the membrane. Thus, semisolid products such as ice cream and some yogurts, and products containing thickeners needed to be diluted using a phosphate-buffered saline-based buffer, pH 7.2. SEB was easily detected at concentrations of 5 g/mL and 500 ng/mL when the LFDs were used. SEB was also reliably detected at concentrations below 5 and 0.25 ng/mL, which may induce serious disease.

Detection of Staphylococcal Enterotoxin B in Spiked Food Samples†

2003 ◽  
Vol 66 (10) ◽  
pp. 1851-1856 ◽  
Author(s):  
LISA C. SHRIVER-LAKE ◽  
YURA S. SHUBIN ◽  
FRANCES S. LIGLER
Keyword(s):  
Social Impact ◽  
Food Poisoning ◽  
Food Samples ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Complex Samples ◽  
Minimal Sample ◽  
Sample Preconcentration ◽  
Array Biosensor ◽  
Enterotoxin B

Contamination of food with infectious agents, intentional or not, is a global concern with far-reaching economic and social impact. Staphylococcal enterotoxins are a major cause of food poisoning, but most methods for the identification of these agents in food require extensive pretreatment or concentration of the sample prior to analysis. The array biosensor was developed as a portable device for the simultaneous analysis of multiple complex samples for multiple targets with minimal sample preparation. In this study, we use an array biosensor to expand and improve on a staphylococcal enterotoxin B (SEB) assay with the ultimate intent of incorporating testing for SEB into a battery of sensitive and convenient assays for food safety validation. In addition to buffer studies, six different types of food samples, including beverages, homogenates of fruit and meat, and carcass washings, were spiked with SEB, incubated for at least 2 h to permit antigen sequestration, and assayed. For all samples, there were differences in fluorescence intensity, but 0.5 ng of SEB per ml could be detected in <20 min with little if any pretreatment and no sample preconcentration.

scholarly journals Screening of hybridoma cell lines secreting monoclonal antibodies specific for staphylococcal enterotoxin B (SEB) derived from Staphylococcus aureus

2016 ◽  
Vol 14 (1) ◽  
pp. 23-28
Author(s):  
Nghiêm Ngọc Minh ◽  
Nguyễn Thị Hoài Thu ◽  
Phạm Thùy Linh ◽  
Thân Đức Dương ◽  
Vũ Thị Thu Hằng ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibodies ◽  
B Lymphocytes ◽  
Food Poisoning ◽  
Gastrointestinal Diseases ◽  
Recombinant Antigen ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B ◽  
High Level

Staphylococcus aureus (S. aureus) produces 11 types of toxins and more than 20 different Staphylococcal enterotoxins (SEs), including SEA to SEE, SEG to SER and SEU. Among them, enterotoxin type B (Staphylococcal enterotoxin B - SEB) is quite heat stable and causes gastrointestinal diseases in food poisoning. The symptoms of SEB intoxication begin with the onset of sudden fever, about 40oC to 41oC, chills, headache, muscle aches and dry cough. Some patients feel shortness of breath and chest pain. Although SEB is not considered lethal, high level of exposure can lead to shock and death. Therefore, a nontoxic SEB recombinant antigen was produced to immunize mice to create B lymphocytes. Myeloma cells were fused with the B lymphocytes to generate  hybridoma lines. The screening of monoclonal antibodies for the SEB antigen was determined by ELISA and Western blot tests. This study demonstrates that an SEB recombinant antigen can immunize a response against SEB in BALB/c mice. The production of monoclonal antibodies will be used to make a rapid detection strip for SEB based on immunochromatography.

Influences of σBandagron expression of staphylococcal enterotoxin B (seb) inStaphylococcus aureus

2004 ◽  
Vol 50 (5) ◽  
pp. 351-360 ◽  
Author(s):  
Katherine A Schmidt ◽  
Niles P Donegan ◽  
William A Kwan, Jr. ◽  
Ambrose Cheung
Keyword(s):  
Fluorescent Protein ◽  
Food Poisoning ◽  
Inverse Correlation ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Gfp Expression ◽  
Enterotoxin B ◽  
Specific Regulation ◽  
Green Fluorescent ◽  
High Level

In Staphylococcus aureus, enterotoxin B (SEB) is a superantigen that activates host interleukins and induces adverse responses, ranging from food poisoning to toxic shock. The alternate sigma factor, σB(SigmaB), and agr are two known regulators of S. aureus. Northern blots of strain COL, a sigB-positive strain, showed an inverse correlation between σBexpression and seb message. seb expression was also measured as a function of a seb promoter linked to green fluorescent protein (GFP) expression in RN6390, COL, and Newman. In sigB mutants of RN6390, SH1000, COL, and Newman, seb promoter activities, as measured by GFP expression, increased relative to the respective parental types but at differing levels, suggesting alternate strain-specific regulation. In agr mutants of RN6390 and Newman, seb promoter activities were intermediate between the high level seen for the sigB mutant and the low level in the sigB active strains. A sigB agr double mutant of RN6390 displayed lower GFP expression than the agr mutant. These results suggest that while σBand agr regulate seb expression in a divergent manner, other activator(s) of seb that depend on sigB expression may be present in S. aureus.Key words: staphylococcal enterotoxin B, σBregulation, agr regulation.

Staphylococcal Enterotoxin B-Specific Electrochemiluminescence and Lateral Flow Device Assays Cross-React with Staphylococcal Enterotoxin D

2014 ◽  
Vol 97 (3) ◽  
pp. 862-867 ◽  
Author(s):  
Sandra M Tallent ◽  
Jennifer Hait ◽  
Reginald W Bennett
Keyword(s):  
Emergency Response ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Lateral Flow ◽  
Faculty Members ◽  
Staphylococcal Enterotoxin B ◽  
Lateral Flow Device ◽  
Partial Activation ◽  
Flow Device ◽  
Enterotoxin B

Abstract Guam school children and faculty members experienced symptoms of vomiting, nausea, abdominal cramps, and diarrhea shortly after eating breakfast prepared by contracted caterers. The first illness was reported within an hour after breakfast, affecting 295 students and two faculty members. Local hospitals treated 130 people, and 61 were admitted for further treatment. Reported symptoms were consistent with staphylococcal food poisoning. Initial food testing using a lateral flow device and electrochemiluminescence method incorrectly implicated staphylococcal enterotoxin B as the causative agent, prompting partial activation of Guam's Emergency Response Center. Traditional ELISAs proved that the food poisoning agent was staphylococcal enterotoxin D. More specific and sensitive assays would have alleviated the issues and confusion that surrounded the reporting and investigation of this outbreak.

scholarly journals Dexamethasone Attenuates Staphylococcal Enterotoxin B-Induced Hypothermic Response and Protects Mice from Superantigen-Induced Toxic Shock

2006 ◽  
Vol 50 (1) ◽  
pp. 391-395 ◽  
Author(s):  
Teresa Krakauer ◽  
Marilyn Buckley
Keyword(s):  
Mouse Model ◽  
Virulence Factors ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Induced Hypothermia ◽  
Staphylococcal Enterotoxin B ◽  
Toxic Shock ◽  
Inflammatory Agent ◽  
Hypothermic Response ◽  
Enterotoxin B

ABSTRACT The superantigenic staphylococcal enterotoxins are important virulence factors and contribute to various diseases, including food poisoning and toxic shock. Dexamethasone, an anti-inflammatory agent, attenuated staphylococcal enterotoxin B (SEB)-induced hypothermia and serum proinflammatory cytokines and improved survival from 0% to 86% in a lethal mouse model of SEB-mediated shock.

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