299. Deoxypentose nucleic acids. Part IV. The electrophoresis of the deoxypentose nucleic acid of calf thymus

1949 ◽  
pp. 1406 ◽  
Author(s):  
J. M. Creeth ◽  
D. O. Jordan ◽  
J. Masson Gulland
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Calf Thymus

scholarly journals Determination of Nucleic Acids Using Fluorescence Probe Sensitized by Microemulsion

2006 ◽  
Vol 89 (6) ◽  
pp. 1609-1616
Author(s):  
Qin Wei ◽  
Hui Zhang ◽  
Bin Du ◽  
Caihong Duan
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Probe ◽  
High Sensitivity ◽  
Binding Mode ◽  
Sperm Dna ◽  
Calf Thymus ◽  
Triton X ◽  
Ct Dna

Abstract Because the fluorescence of azur A can be quenched by adding nucleic acid, a sensitive fluorometric method for determination of nucleic acids at nanogram levels was established. Using optimal conditions, the calibration curves were linear in the range of 06.0 μg/mL for calf thymus deoxyribonucleic acid (ct DNA) and 07.0 μg/mL for herring sperm DNA (hs DNA). The limits of determination were 3.5 and 3.8 ng/mL, respectively, which shows the high sensitivity of this method. Triton X-100 microemulsion was applied as a sensitive media to enhance the sensitivity. The binding mode concerning the interactions of azur A with nucleic acids was also studied and the association constant with different binding numbers was obtained. The method has been applied to the determination of nucleic acid in both synthetic and real samples, such as cauliflower and pork liver, with satisfactory results.

SPECTROPHOTOMETRIC ANALYSIS OF NUCLEIC ACIDS IN PROTEIN SOLUTIONS AND IN CRUDE ORGAN EXTRACTS

1955 ◽  
Vol 33 (1) ◽  
pp. 1010-1017
Author(s):  
E. Annau
Keyword(s):  
Absorption Spectrum ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mouse Liver ◽  
Spectrophotometric Analysis ◽  
Protein Solutions ◽  
Spectrophotometric Procedure ◽  
Acid Protein ◽  
Calf Thymus ◽  
Nucleoprotein Complexes

A spectrophotometric procedure has been presented by which an absorption spectrum, essentially characteristic for nucleic acids, could be obtained from mixed nucleic acid protein solutions, and mouse liver extracts. To examine the efficiency of the method for nucleoprotein complexes, spectra from purified calf thymus nucleohiston were prepared showing the absorption curves of both of its components: nucleic acids and histon.

Photoreactivity (3655 Å) of various skin-photosensitizing furocoumarins with nucleic acids

1969 ◽  
Vol 24 (3) ◽  
pp. 307-314 ◽  
Author(s):  
F. Dall’Acqua ◽  
S. Marciani ◽  
G. Rodighiero
Keyword(s):  
Double Bond ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Room Temperature ◽  
Addition Reaction ◽  
The Other ◽  
Temperature Influence ◽  
Dna And Rna ◽  
Calf Thymus ◽  
Pyrimidine Bases

The photoreaction at 3655 A between skin-photosensitizing furocoumarins and DNA leads to a photo-C4-cyclo-addition reaction in which the 5 - 6 double bond of the pyrimidine bases and the 3 -4 or the 4' -5′ double bond of the furocoumarins are involved. In continuing the research in this field, the authors have investigated the photoreactions (3655 A) of psoralen, xanthotoxin (8-methoxy-psoralen), bergapten (5-methoxy-psoralen) and 8-methyl-psoralen with native calf thymus DNA. with heat-denatured DNA and with ribosomal yeast-RNA, using labelled furocoumarins and examining also the influence of the temperature at which the irradiation was made. At room temperature (22° -30°C) psoralen photoreacts in almost equally extent with each of the three nucleic acid samples, while xanthotoxin, bergapten and 8-methyl-psoralen photoreact to a greater extent with native DNA than with denatured DNA and RNA.The temperature during the irradiation (in the range examined: 2° - 30°C) does not affect the rate of the photoreactions between the four furocoumarins and native DNA, whereas it has an evident influence on the photoreactions with denatured DNA and with RNA. In the photoreaction of psoralen this influence concerns the double bond (3 - 4 or 4' -5′) of the furocoumarin which is engaged in the C4-cycloaddition to pyrimidine bases. In the case of the other furocoumarins, the rate of the photoreactions is affected: at 2° - 8°C it is greater than at 30 °C. The preliminary formation (in the dark) of a complex between the furocoumarins and the macromolecules is involved in this temperature influence.

SPECTROPHOTOMETRIC ANALYSIS OF NUCLEIC ACIDS IN PROTEIN SOLUTIONS AND IN CRUDE ORGAN EXTRACTS

1955 ◽  
Vol 33 (6) ◽  
pp. 1010-1017 ◽  
Author(s):  
E. Annau
Keyword(s):  
Absorption Spectrum ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mouse Liver ◽  
Spectrophotometric Analysis ◽  
Protein Solutions ◽  
Spectrophotometric Procedure ◽  
Acid Protein ◽  
Calf Thymus ◽  
Nucleoprotein Complexes

A spectrophotometric procedure has been presented by which an absorption spectrum, essentially characteristic for nucleic acids, could be obtained from mixed nucleic acid protein solutions, and mouse liver extracts. To examine the efficiency of the method for nucleoprotein complexes, spectra from purified calf thymus nucleohiston were prepared showing the absorption curves of both of its components: nucleic acids and histon.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

Sign in / Sign up

Export Citation Format

Share Document