A chromatographic preparation of ox growth hormone
Keyword(s):
1. A method is described for the chromatographic preparation of ox growth hormone. It involves chromatography of an extract of anterior pituitary lobes on DEAE-cellulose, followed by rechromatography on a dextran gel of low cross-linkage (Sephadex G-100). 2. The product is highly active in growth-hormone assays, and is obtained in good yield. It was homogeneous by several criteria, but showed some heterogeneity on starch-gel electrophoresis. 3. The molecular weight of the hormone was estimated from its behaviour on gel-filtration columns under various conditions. Evidence that the hormone may dissociate into sub-units under some conditions is presented.
1967 ◽
Vol 6
(2)
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pp. 69-89
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1970 ◽
Vol 30
(2)
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pp. 509-517
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1974 ◽
Vol 41
(2)
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pp. 269-282
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Keyword(s):
1964 ◽
Vol 161
(983)
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pp. 153-167
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