A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase

1. Inhibition of ox liver glutamate dehydrogenase with N-(N′-acetyl-4[35S]-sulphamoylphenyl)maleimide (ASPM) is more specific at pH7·3 than at pH6·9. At pH7·3 inhibition accompanies the incorporation at 1 mole of ASPM residues into about 53000g. of protein. 2. Digestion of the modified protein with chymotrypsin and trypsin yields a unique radioactive peptide. 3. Acid hydrolysis of 1 mole of this peptide yields 1 mole of N∈-succin-2-yl-lysine. The ∈-amino group of a lysyl residue is thus the site of modification of the protein. 4. The sequence containing the modified lysyl residue is: [Formula: see text] where Asx respresents either aspartic acid or asparagine.

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The liberation of aspartic acid during the acid hydrolysis of proteins

Biochemical Journal ◽

10.1042/bj0580227 ◽

1954 ◽

Vol 58 (2) ◽

pp. 227-231 ◽

Cited By ~ 22

Author(s):

S. Blackburn ◽

G. R. Lee

Keyword(s):

Acid Hydrolysis ◽

Aspartic Acid ◽

Hydrolysis Of

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Biosynthesis of Lycomarasmin

Canadian Journal of Chemistry ◽

10.1139/v73-587 ◽

1973 ◽

Vol 51 (23) ◽

pp. 3943-3949 ◽

Cited By ~ 7

Author(s):

C. R. POPPLESTONE ◽

A. M. Unrau

Keyword(s):

Carbon Source ◽

Liquid Medium ◽

Fusarium Oxysporum ◽

Acid Hydrolysis ◽

Aspartic Acid ◽

Pyruvic Acid ◽

Tracer Studies ◽

Direct Route ◽

Carbon Fragment ◽

Hydrolysis Of

Cultures of Fusarium oxysporum Schl. emend. Sny. et Hans. f lycopersici (Sacc.) Sny. et Hans. grown on a liquid medium with glucose as the principal carbon source produce, among other products, the phytotoxin lycomarasmin. Acid hydrolysis of lycomarasmin results in the formation of aspartic acid, glycine, and pyruvic acid. Tracer studies showed that glycine-U-I4C, L-serine-U-14C, DL-aspartic acid-4-14C, DL-alanine-1-14C, and glucose-U-14C served as relatively efficient precursors of the lycomarasmin molecule. DL-Aspartic acid-4-I4C was incorporated into the 4-carbon fragment without label scrambling. Glycine was found to be the most efficient precursor of the 2-carbon fragment while glucose afforded the most efficient and direct route for the 3-carbon fragment.

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Synthesis of 9-(2-Phosphinomethoxyethyl)adenine and Related Compounds

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19941870 ◽

1994 ◽

Vol 59 (8) ◽

pp. 1870-1878 ◽

Cited By ~ 6

Author(s):

Petr Alexander ◽

Antonín Holý ◽

Milena Masojídková

Keyword(s):

Acid Hydrolysis ◽

Phosphorus Atom ◽

Amino Group ◽

Protecting Group ◽

Hydrolysis Of ◽

Related Compounds

Alkyl 2-chloroethoxymethyl(diethoxymethyl)phosphinates VII and XIII were prepared by reaction of silyl esters of dialkoxymethylphosphinic acid with 2-chloroethyl chloromethyl ether. Adenine was alkylated with VII and XIII to give [2-(adenin-9-yl)ethoxy]methyl(diethoxymethyl)phosphinates VIII and XIV, bearing the dialkoxymethyl protecting group on the phosphorus atom. Acid hydrolysis of compounds VIII and XIV afforded 9-(2-phosphinoethoxymethyl)adenine (X). Alkyl dialkoxymethylphosphinates V and XI reacted with paraformaldehyde to give hydroxymethylphosphinates XV and XIX which were converted into the synthons XVI, XVII and XVIII capable of introducing a protected hydroxymethylphosphino group on a hydroxy or amino group.

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Assisted hydrolysis of the nitrile group of 2-aminoadamantane-2-carbonitrile

Canadian Journal of Chemistry ◽

10.1139/v87-351 ◽

1987 ◽

Vol 65 (9) ◽

pp. 2114-2117 ◽

Cited By ~ 12

Author(s):

Martino Paventi ◽

Francis L. Chubb ◽

John T. Edward

Keyword(s):

Amino Acid ◽

Acid Hydrolysis ◽

Alkaline Solution ◽

Mineral Acid ◽

Nitrile Group ◽

Amino Group ◽

Hydrolysis Of

Attempts to hydrolyse the nitrile group of 2-aminoadamantane-2-carbonitrile by mineral acid or alkali have been unsuccessful. However, treatment of the aminonitrile with benzaldehyde in alkaline solution gives the benzal derivative of the α-aminoamide, readily hydrolysed to the α-aminoamide. Alternatively, benzoylation of the amino group followed by acid hydrolysis gives successively the α-benzamido acid and the α-amino acid. Possible mechanisms for these facilitated hydrolyses are advanced.

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Regulation of acid hydrolysis of sucrose in acid lime juice sac cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810108.x ◽

1991 ◽

Vol 81 (1) ◽

pp. 51-54 ◽

Cited By ~ 1

Author(s):

Ed Echeverria

Keyword(s):

Acid Hydrolysis ◽

Lime Juice ◽

Acid Lime ◽

Hydrolysis Of

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DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

Acta Endocrinologica ◽

10.1530/acta.0.0410234 ◽

1962 ◽

Vol 41 (2) ◽

pp. 234-246 ◽

Cited By ~ 5

Author(s):

H. J. van der Molen

Keyword(s):

Infrared Spectrum ◽

Quantitative Determination ◽

Acid Hydrolysis ◽

Chromatographic Separation ◽

Pure Form ◽

Infrared Spectrometry ◽

Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

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