Biosynthesis of Lycomarasmin

Cultures of Fusarium oxysporum Schl. emend. Sny. et Hans. f lycopersici (Sacc.) Sny. et Hans. grown on a liquid medium with glucose as the principal carbon source produce, among other products, the phytotoxin lycomarasmin. Acid hydrolysis of lycomarasmin results in the formation of aspartic acid, glycine, and pyruvic acid. Tracer studies showed that glycine-U-I4C, L-serine-U-14C, DL-aspartic acid-4-14C, DL-alanine-1-14C, and glucose-U-14C served as relatively efficient precursors of the lycomarasmin molecule. DL-Aspartic acid-4-I4C was incorporated into the 4-carbon fragment without label scrambling. Glycine was found to be the most efficient precursor of the 2-carbon fragment while glucose afforded the most efficient and direct route for the 3-carbon fragment.

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The liberation of aspartic acid during the acid hydrolysis of proteins

Biochemical Journal ◽

10.1042/bj0580227 ◽

1954 ◽

Vol 58 (2) ◽

pp. 227-231 ◽

Cited By ~ 22

Author(s):

S. Blackburn ◽

G. R. Lee

Keyword(s):

Acid Hydrolysis ◽

Aspartic Acid ◽

Hydrolysis Of

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BIOSYNTHESIS OF NICOTINIC ACID BY FUSARIUM OXYSPORUM SCHLECHT

Canadian Journal of Biochemistry ◽

10.1139/o67-228 ◽

1967 ◽

Vol 45 (12) ◽

pp. 1953-1959 ◽

Cited By ~ 7

Author(s):

D. Desaty ◽

L. C. Vining

Keyword(s):

Nicotinic Acid ◽

Fusarium Oxysporum ◽

Acid Hydrolysis ◽

Growth Phase ◽

Sodium Acetate ◽

Fusaric Acid ◽

Early Growth ◽

Pyridine Derivatives ◽

Early Growth Phase ◽

Hydrolysis Of

When the fungus Fusarium oxysporum was cultured on a glucose–nitrate–salts medium, synthesis of nicotinic acid occurred only during the early growth phase and preceded the accumulation of fusaric acid. Radioactivity from DL-tryptophan-benzene ring-14C was efficiently incorporated into nicotinic acid obtained by acid hydrolysis of the mycelium, whereas DL-tryptophan-β-14C, L-aspartate-U-14C, and sodium acetate-2-14C were poor precursors. Under the conditions of these experiments, all four substrates were poor precursors of fusaric acid. It is concluded that different pathways are used in the biosynthesis of these two pyridine derivatives, the route to nicotinic acid probably involving metabolism of tryptophan via the reactions known to occur in Neurospora crassa.

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Tracer studies on ester hydrolysis. Part II. The acid hydrolysis of tert.-butyl acetate

Journal of the Chemical Society (Resumed) ◽

10.1039/jr9550001522 ◽

1955 ◽

pp. 1522 ◽

Cited By ~ 9

Author(s):

C. A. Bunton ◽

J. L. Wood

Keyword(s):

Acid Hydrolysis ◽

Butyl Acetate ◽

Ester Hydrolysis ◽

Tracer Studies ◽

Tert Butyl ◽

Hydrolysis Of

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A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase

Biochemical Journal ◽

10.1042/bj1110689 ◽

1969 ◽

Vol 111 (5) ◽

pp. 689-694 ◽

Cited By ~ 39

Author(s):

J. J. Holbrook ◽

Renate Jeckel

Keyword(s):

Acid Hydrolysis ◽

Glutamate Dehydrogenase ◽

Aspartic Acid ◽

Amino Group ◽

Hydrolysis Of ◽

Lysyl Residue

1. Inhibition of ox liver glutamate dehydrogenase with N-(N′-acetyl-4[35S]-sulphamoylphenyl)maleimide (ASPM) is more specific at pH7·3 than at pH6·9. At pH7·3 inhibition accompanies the incorporation at 1 mole of ASPM residues into about 53000g. of protein. 2. Digestion of the modified protein with chymotrypsin and trypsin yields a unique radioactive peptide. 3. Acid hydrolysis of 1 mole of this peptide yields 1 mole of N∈-succin-2-yl-lysine. The ∈-amino group of a lysyl residue is thus the site of modification of the protein. 4. The sequence containing the modified lysyl residue is: [Formula: see text] where Asx respresents either aspartic acid or asparagine.

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Regulation of acid hydrolysis of sucrose in acid lime juice sac cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810108.x ◽

1991 ◽

Vol 81 (1) ◽

pp. 51-54 ◽

Cited By ~ 1

Author(s):

Ed Echeverria

Keyword(s):

Acid Hydrolysis ◽

Lime Juice ◽

Acid Lime ◽

Hydrolysis Of

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DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

Acta Endocrinologica ◽

10.1530/acta.0.0410234 ◽

1962 ◽

Vol 41 (2) ◽

pp. 234-246 ◽

Cited By ~ 5

Author(s):

H. J. van der Molen

Keyword(s):

Infrared Spectrum ◽

Quantitative Determination ◽

Acid Hydrolysis ◽

Chromatographic Separation ◽

Pure Form ◽

Infrared Spectrometry ◽

Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

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