scholarly journals The biosynthesis of cis-4-hydroxy-L-proline in sandal (Santalum album L.)

1970 ◽  
Vol 117 (5) ◽  
pp. 1015-1017 ◽  
Author(s):  
R Kuttan ◽  
A N Radhakrishnan
Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 118
Author(s):  
Manoj Kumar Tripathi ◽  
Niraj Tripathi ◽  
Sushma Tiwari ◽  
Gyanendra Tiwari ◽  
Nishi Mishra ◽  
...  

Santalum album (L.) is a prized tropical tree species of high therapeutic and industrial importance. The wood of these naturally grown plants is extensively harvested to acquire therapeutically important metabolite santalol and be used for additional functions such as in wood statuette industries. Due to high demand, it is crucial to maintain a sufficient plant population. An easy protocol for establishing cell suspension culture initiated from the loose embryogenic callus mass of sandalwood was realized by shifting 6–8-week-old morphogenic calli acquired from the mature embryonic axis and cotyledon explant cultures in fluid media. The asynchronous embryogenic cultures were sloughed with clumps of flourishing cell clumps and embryos of various progressive phases along with diffident non-embryogenic tissues. The frequency of embryo proliferation was evidenced to determinethe expansion pace of embryogenic masses under diverse conditions. The intonation of initiation and creation of cell suspension was under the directive of the influence of exogenous plant growth regulators amended in the nutrient medium at different concentrations and combinations. Maximum relative growth rate (386%) and clumps/embryoids in elevated integers (321.44) were accomplished on MS nutrient medium fortified with 2.0 mg L−1 2,4-D in association with 0.5 mg L−1 BA and 30.0 g L−1 sucrose raised from mature embryonic axis-derived calli. Plantlet regeneration in higher frequency (84.43%) was evidenced on MS medium amended with 1.0 mg L−1 each of TDZ and GA3 in conjunction with 0.5 mg L−1 NAA and 20.0 g L−1 sucrose. Mature embryonic axis-derived calli were found to be constantly better than mature cotyledon-derived calli for raising profitable and reproducible cell suspension cultures. Regenerants displayed normal growth and morphology and were founded successfully in the external environment after hardening.


2021 ◽  
Vol 183 ◽  
pp. 112610
Author(s):  
Xinhua Zhang ◽  
Jaime A. Teixeira da Silva ◽  
Meiyun Niu ◽  
Ting Zhang ◽  
Huanfang Liu ◽  
...  

1996 ◽  
Vol 32 (3) ◽  
pp. 123-128 ◽  
Author(s):  
K. Sankara Rao ◽  
N. K. Chrungoo ◽  
Amares Sinha

2011 ◽  
Vol 8 (1) ◽  
pp. 51-58
Author(s):  
Eritrina Windyarini ◽  
Illa Anggraeni

2021 ◽  
Author(s):  
Tanzeem Fatima ◽  
Rangachari Krishnan ◽  
Ashutosh Srivastava ◽  
Vageeshbabu S. Hanur ◽  
M. Srinivasa Rao

East Indian Sandalwood (Santalum album L.) is highly valued for its heartwood and its oil. There have been no efforts to comparative study of high and low oil yielding genetically identical sandalwood trees grown in similar climatic condition. Thus we intend to study a genome wide transcriptome analysis to identify the corresponding genes involved in high oil biosynthesis in S. album. In this study, 15 years old S. album (SaSHc and SaSLc) genotypes were targeted for analysis to understand the contribution of genetic background on high oil biosynthesis in S. album. A total of 28,959187 and 25,598869 raw PE reads were generated by the Illumina sequencing. 2.12 million and 1.811 million coding sequences were obtained in respective accessions. Based on the GO terms, functional classification of the CDS 21262, & 18113 were assigned into 26 functional groups of three GO categories; (4,168; 3,641) for biological process (5,758;4,971) cellular component and (5,108;4,441) for molecular functions. Total 41,900 and 36,571 genes were functionally annotated and KEGG pathways of the DEGs resulted 213 metabolic pathways. In this, 14 pathways were involved in secondary metabolites biosynthesis pathway in S. album. Among 237 cytochrome families, nine groups of cytochromes were participated in high oil biosynthesis. 16,665 differentially expressed genes were commonly detected in both the accessions (SaHc and SaSLc). The results showed that 784 genes were upregulated and 339 genes were downregulated in SaHc whilst 635 upregulated 299 downregulated in SaSLc S. album. RNA-Seq results were further validated by quantitative RT-PCR. Maximum Blast hits were found to be against Vitis vinifera. From this study we have identified additional number of cytochrome family in SaHc. The accessibility of a RNA-Seq for high oil yielding sandalwood accessions will have broader associations for the conservation and selection of superior elite samples/populations for further genetic improvement program.


Author(s):  
Shubhashree Sahu V. Maheswarappa ◽  
Ramakrishna Hegde N. Kencharaddi ◽  
B. N. Sathish

Sandalwood (Santalum album L.) is one of the most valuable commercially cultivated aromatic species throughout the world. However, the availability of quality planting stock isthe current bottleneck in its establishment on large scale. The major problems, noticed during quality planting stock production in nurseries are scarce knowledge about host-parasite relationship and use of appropriate potting mixture apart from its seed germination. In this regard two experiments were carried out at the College of Forestry, Ponnampet during 2020-2021 to identify suitable host plant and potting mixture. Growth parameters were recorded after 90, 180 and 270 days of transplanting sandal seedlings into treatments. After 270 days of transplanting, the host plant A. lebbeck recorded higher survival of sandal seedlings (81.89 %) and the host plant C. junghuhniana had higher height growth (10.40 cm) with collar diameter 2.33mm. Potting mixture sand, soil, vermicompost, G. intraradices with C. Junghuhninana found effective for height (18.25 cm) and collar diameter (2.58 mm).


2020 ◽  
Vol 7 (1) ◽  
pp. 65
Author(s):  
Barnadus Nggahu Lindi Djawa ◽  
Ni Luh Arpiwi ◽  
Sang Ketut Sudirga

Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bawang merah, air kelapa dan skarifikasi terhadap pertumbuhan cendana. Manfaat dari penilitian ini untuk menjawab permasalahan pembudidyaan tanaman cendana di Pulau Sumba. Penelitian ini mengunakan rancangan acak lengkap dengan enam perlakuan dan tiga kali ulangan pada masing-masing unit percobaan. Parameter yang diukur dalam penelitian ini persentase bibit tumbuh, tinggi tanaman, jumlah daun, panjang daun, dan lebar daun. Analisis data mengunakan ANOVA dengan taraf uji 5% bila terdapat beda nyata pada salah satu faktor dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) pada taraf uji 5% menggunakan analisis statistik SPSS. Hasil penelitian menujukkan perlakuan ekstrak bawang merah dan metode skarifikasi memberikan hasil yang paling tinggi persentase bibit tumbuh sebesar 70%. Perlakuan kombinasi skarifikasi dengan air kelapa muda memberikan hasil yang paling tinggi terhadap pertumbuhan tanaman cendana. Untuk itu penulis merekomendasikan kepada petani cendana menggunakan ekstrak bawang merah atau skarifikasi untuk meningkatkan persentase bibit tumbuh sedangkan untuk memacu pertubuhan tanaman cendana menggunakan kombinasi skarifikasi dengan air kelapa muda.   Kata kunci: bawang merah, air kelapa, skarifikasi


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