scholarly journals Liver enzyme induction by 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) is accompanied by an increase in the specific activity of elongation factor 1

1978 ◽  
Vol 172 (3) ◽  
pp. 479-486 ◽  
Author(s):  
E T Young ◽  
D M Nicholls
Keyword(s):  
Molecular Weight ◽  
Enzyme Induction ◽  
Sucrose Gradient ◽  
Binding Assay ◽  
Specific Activity ◽  
Elongation Factor ◽  
Low Molecular Weight ◽  
Binding Factor ◽  
The Difference ◽  
Elongation Factor 1

Homogenates of liver were obtained from control rats and from rats that had received DDT [1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane]. The postmicrosomal supernatant fractions were used for the purification of elongation factor 1 by hydroxyapatite chromatography and phosphocellulose chromarography. The amount of binding factor present was essentially the same for both groups of animals, but the specific activity, as measured by the binding assay, was about twice as high in the DDT-treated preparations. After sucrose-gradient sedimentation, the difference in specific activity was found to reside in the low-molecular-weight (50000) form of elongation factor 1. The implications of an increased reactivity of elongation factor 1 during the induction of membrane enzymes are discussed.

scholarly journals The Purification of Low Molecular Weight Form of Polypeptide Elongation Factor 1 from Pig Liver

1974 ◽  
Vol 249 (15) ◽  
pp. 5008-5010
Author(s):  
Kentaro Iwasaki ◽  
Shigekazu Nagata ◽  
Kiyohisa Mizumoto ◽  
Yoshito Kaziro
Keyword(s):  
Molecular Weight ◽  
Elongation Factor ◽  
Low Molecular Weight ◽  
Pig Liver ◽  
Elongation Factor 1 ◽  
Molecular Weight Form

ANALISA KANDUNGAN BETA-GLUKAN LARUT AIR DAN LARUT ALKALI DARI TUBUH BUAH JAMUR TIRAM (Pleurotus ostreatus) DAN SHIITAKE (Lentinus edodes)

2013 ◽  
Vol 13 (3) ◽  
Author(s):  
Netty Widyastuti ◽  
Teguh Baruji ◽  
Henky Isnawan ◽  
Priyo Wahyudi ◽  
Donowati Donowati
Keyword(s):  
Molecular Structure ◽  
Molecular Weight ◽  
Immune System ◽  
Pleurotus Ostreatus ◽  
Water Soluble ◽  
Low Molecular Weight ◽  
Lentinus Edodes ◽  
Beta Glucan ◽  
Oyster Mushrooms ◽  
The Difference

Beta glucan is a polysaccharide compound, generally not soluble inwater and resistant to acid. Beta glucan is used as an immunomodulator (enhancing the immune system) in mammals is usually a beta-glucan soluble in water, easily absorbed and has a low molecular weight. Several example of beta-glucan such as cellulose (β-1 ,4-glucan), lentinan (β-1 0.6-glucan) and (β-1 ,3-glucan), pleuran (β-1, 6 and β-1 ,3-glucan) are isolated from species of fungi Basidiomycota include mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes).The purpose of thisresearch activity is to obtain beta-glucan compound that can be dissolved in water and in alkali derived from fungi Basidiomycota, i.e, Oyster mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes). The result of beta-glucan compared to characterize the resulting beta glucan that is molecular structure . The difference of beta glucan extraction is based on the differences in solubility of beta-glucan. Beta glucan could be water soluble and insoluble water.

scholarly journals The interrelations between high- and low-molecular-weight forms of normal and mutant (Krabbe-disease) galactocerebrosidase

1980 ◽  
Vol 189 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Yoav Ben-Yoseph ◽  
Melinda Hungerford ◽  
Henry L. Nadler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Active Form ◽  
Sodium Dodecyl ◽  
Krabbe Disease ◽  
Low Molecular Weight ◽  
Molecular Weight Form

Galactocerebrosidase (β-d-galactosyl-N-acylsphingosine galactohydrolase; EC 3.2.1.46) activity of brain and liver preparations from normal individuals and patients with Krabbe disease (globoid-cell leukodystrophy) have been separated by gel filtration into four different molecular-weight forms. The apparent mol.wts. were 760000±34000 and 121000±10000 for the high- and low-molecular-weight forms (peaks I and IV respectively) and 499000±22000 (mean±s.d.) and 256000±12000 for the intermediate forms (peaks II and III respectively). On examination by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, the high- and low-molecular-weight forms revealed a single protein band with a similar mobility corresponding to a mol.wt. of about 125000. Antigenic identity was demonstrated between the various molecular-weight forms of the normal and the mutant galactocerebrosidases by using antisera against either the high- or the low-molecular-weight enzymes. The high-molecular-weight form of galactocerebrosidase was found to possess higher specific activity toward natural substrates when compared with the low-molecular-weight form. It is suggested that the high-molecular-weight enzyme is the active form in vivo and an aggregation process that proceeds from a monomer (mol.wt. approx. 125000) to a dimer (mol.wt. approx. 250000) and from the dimer to either a tetramer (mol.wt. approx. 500000) or a hexamer (mol.wt. approx. 750000) takes place in normal as well as in Krabbe-disease tissues.

scholarly journals Selection of inorganic-based fertilizers in forward osmosis for water desalination

2015 ◽  
Vol 4 (2) ◽  
pp. 319-329 ◽  
Author(s):  
Tripti Mishra ◽  
RK Srivastava
Keyword(s):  
Molecular Weight ◽  
Water Flux ◽  
Forward Osmosis ◽  
Direct Application ◽  
Water Desalination ◽  
Performance Ratio ◽  
Low Molecular Weight ◽  
Inorganic Fertilizers ◽  
The Difference ◽  
Selection Of

The current study aims at the selection of an appropriate draw solute for forward osmosis process. Separation and recovery of the draw solute are the major criteria for the selection of draw solute for forward osmosis process. Therefore in this investigation six inorganic fertilizers draws solute were selected. The selections of inorganic fertilizers as draw solute eliminate the need of removal and recovery of draw solute from the final product. The final product water of forward osmosis process has direct application in agricultural as nutrient rich water for irrigation. These inorganic fertilizers were tested based on their water extraction (water flux) capacity. This experimental water flux was compared with the observed water flux. It was noted that the observed water flux is much higher than the attained experimental water flux. The difference of these two fluxes was used to calculate the performance ratio of each selected fertilizer. Highest performance ratio was shown by low molecular weight compound ammonium nitrate (22.73) and potassium chloride (21.03) at 1 M concentration, whereas diammonium phosphate (DAP) which has highest molecular weight among all the selected fertilizer show the lowest performance ratio (10.02) at 2 M concentration. DOI: http://dx.doi.org/10.3126/ije.v4i2.12660 International Journal of Environment Vol.4(2) 2015: 319-329

scholarly journals Low-temperature dynamics in amorphous polymers and low-molecular-weight glasses—what is the difference?

2011 ◽  
Vol 13 (5) ◽  
pp. 1843-1848 ◽  
Author(s):  
Ivan Yu. Eremchev ◽  
Yury G. Vainer ◽  
Andrei V. Naumov ◽  
Lothar Kador
Keyword(s):  
Molecular Weight ◽  
Low Temperature ◽  
Amorphous Polymers ◽  
Low Molecular Weight ◽  
Temperature Dynamics ◽  
The Difference

Influence of Botanical Species and Geographical Origin on the Content of Low Molecular Weight Phenolic Compounds of Woods Used in Portuguese Cooperage

Holzforschung ◽  
2000 ◽  
Vol 54 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Sara Canas ◽  
M. Conceição Leandro ◽  
M. Isabel Spranger ◽  
A. Pedro Belchior
Keyword(s):  
Molecular Weight ◽  
Phenolic Compounds ◽  
Geographical Origin ◽  
Castanea Sativa ◽  
Total Content ◽  
Low Molecular Weight ◽  
Qualitative And Quantitative ◽  
Botanical Species ◽  
The Difference ◽  
Chestnut Wood

Summary The influence of botanical species (Quercus and Castanea sativa) and geographical origin (Portugal—three different sites, France and North America) on the qualitative and quantitative content of some extractable low molecular weight phenolic compounds was assessed by HPLC. Chestnut wood had the highest total content of low molecular weight phenolic compounds, followed by the Portuguese oaks and the French oaks, whereas the American oak had the lowest content of these compounds. The contents of phenolic acids, phenolic aldehydes, scopoletin and umbelliferone were significantly different among the studied woods. Both the botanical species and the geographical origin affect the content of low molecular weight organic compounds of woods used in Portuguese cooperage. The results also show that the botanical species seem to be more important than the geographical origin to explain the difference in the wood phenolic composition. So, the separation of trees according to their origin is of considerable interest for coopers and winemakers to choose wisely the woods for the ageing of brandies.

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