An improved spectrophotometric assay for leucine aminopeptidase
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A sensitive assay to determine the activity of leucine aminopeptidase (EC 3.4.11.1), using L-leucine thiobenzyl ester as substrate, was developed. Hydrolysis of the ester by leucine aminopeptidase can be monitored in the presence of 5,5-dithiobis-(2-nitrobenzoic acid) by continuous spectrophotometric measurement at 412 nm. Comparison with some amide substrates showed that the thiol ester provides a much more sensitive assay, its specificity constant (Vmax./Km) being some 3000-fold higher than that of leucine p-nitroanilide.
1985 ◽
Vol 50
(4)
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pp. 845-853
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2007 ◽
Vol 227
(1)
◽
pp. 159-165
◽
1965 ◽
Vol 19
◽
pp. 1566-1574
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