Thiol ester derivatives of leucine as substrates for leucine aminopeptidase

1987 ◽  
Vol 15 (2) ◽  
pp. 232-233
Author(s):  
R. E. BEATTIE ◽  
B. WALKER ◽  
D. T. ELMORE ◽  
C. H. WILLIAMS
Keyword(s):  
Leucine Aminopeptidase ◽  
Thiol Ester ◽  
Derivatives Of

Unusual activity pattern of leucine aminopeptidase inhibitors based on phosphorus containing derivatives of methionine and norleucine

2010 ◽  
Vol 26 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Jan Pícha ◽  
Radek Liboska ◽  
Miloš Buděšínský ◽  
Jiři Jiráček ◽  
Małgorzata Pawełczak ◽  
...  
Keyword(s):  
Activity Pattern ◽  
Leucine Aminopeptidase ◽  
Phosphorus Containing ◽  
Derivatives Of ◽  
Aminopeptidase Inhibitors

scholarly journals An improved spectrophotometric assay for leucine aminopeptidase

1987 ◽  
Vol 242 (1) ◽  
pp. 281-283 ◽  
Author(s):  
R E Beattie ◽  
D J S Guthrie ◽  
D T Elmore ◽  
C H Williams ◽  
B Walker
Keyword(s):  
Leucine Aminopeptidase ◽  
Spectrophotometric Assay ◽  
Sensitive Assay ◽  
Spectrophotometric Measurement ◽  
Thiol Ester ◽  
Nitrobenzoic Acid ◽  
Specificity Constant ◽  
Hydrolysis Of

A sensitive assay to determine the activity of leucine aminopeptidase (EC 3.4.11.1), using L-leucine thiobenzyl ester as substrate, was developed. Hydrolysis of the ester by leucine aminopeptidase can be monitored in the presence of 5,5-dithiobis-(2-nitrobenzoic acid) by continuous spectrophotometric measurement at 412 nm. Comparison with some amide substrates showed that the thiol ester provides a much more sensitive assay, its specificity constant (Vmax./Km) being some 3000-fold higher than that of leucine p-nitroanilide.

Notes: Mammalian Pheromone Studies, VI. Compounds from the Preorbital Gland of the Blue Duiker, Cephalophus monticola

1987 ◽  
Vol 42 (11-12) ◽  
pp. 1355-1357 ◽  
Author(s):  
B. V. Burger ◽  
P. J. Pretorius
Keyword(s):  
Volatile Compounds ◽  
Aromatic Compounds ◽  
Thiol Ester ◽  
Aromatic Thiol ◽  
Cephalophus Monticola ◽  
Branched Chain ◽  
Blue Duiker ◽  
Derivatives Of ◽  
Preorbital Secretion

Forty five volatile compounds, mostly straight- and branched-chain alcohols and ketones, as well as derivatives of two of these alcohols, two y-lactones, an aromatic thiol ester and other simple aromatic compounds, have been identified in the preorbital secretion of the blue duiker, Cephalophus monticola.

scholarly journals Inhibition of metallo-β-lactamases by a series of thiol ester derivatives of mercaptophenylacetic acid

2006 ◽  
Vol 157 (1) ◽  
pp. 171-175 ◽  
Author(s):  
David J. Payne ◽  
John H. Bateson ◽  
Brian C. Gasson ◽  
Teresa Khushi ◽  
David Proctor ◽  
...  
Keyword(s):  
Thiol Ester ◽  
Derivatives Of

scholarly journals Study of stability of proline-containing derivatives of dopamine and serotonin in the biological media in vitro experiments

2019 ◽  
Vol 65 (6) ◽  
pp. 498-506 ◽  
Author(s):  
K.V. Shevchenko ◽  
L.A. Andreeva ◽  
I.Yu. Nagaev ◽  
V.P. Shevchenko ◽  
N.F. Myasoedov
Keyword(s):  
Leucine Aminopeptidase ◽  
Biologically Active ◽  
Carboxypeptidase Y ◽  
Vital Activity ◽  
Prolonged Action ◽  
Carboxypeptidase B ◽  
The Stability ◽  
First Time ◽  
Derivatives Of

Boc-Gly-Pro-DP, Z-Gly-Pro-DP, LA-Gly-Pro-DP, Boc-Gly-Pro-Srt, Z-Gly-Pro-Srt were synthesized for the first time. The stability of these compounds in the presence of leucine aminopeptidase, carboxypeptidase Y, carboxypeptidase B and proline endopeptidase (PEP) was determined. It turned out that the compounds are stable in the presence of aminopeptidases and carboxypeptidases. In the presence of PEP, dopamine (DP) and serotonin (Srt) are cleaved from the synthesized preparations. Thus, new proline-containing Srt and DP derivatives were obtained, Srt and DP could be gradually released from them. This suggest the possibility of a prolonged action of these biologically active compounds on the vital activity of cells and, consequently, of the whole organism.

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