scholarly journals Cloning and characterization of the rat bone sialoprotein gene promoter

1993 ◽  
Vol 289 (3) ◽  
pp. 625-629 ◽  
Author(s):  
J J Li ◽  
J Sodek
Keyword(s):  
Gene Promoter ◽  
Bone Sialoprotein ◽  
Regulation Of Transcription ◽  
Flanking Sequence ◽  
Dihydroxyvitamin D3 ◽  
Tissue Specific ◽  
1,25 Dihydroxyvitamin D3 ◽  
Tata Element ◽  
Ccaat Box ◽  
Rat Bone

To study the transcriptional regulation of the rat bone sialoprotein (BSP) gene, the nucleotide sequence of a approximately 1 kb HindIII/KpnI subfragment from a genomic clone containing the 5′ flanking sequence, exon 1 and part of intron 1 was determined and the transcription start site defined. This region includes an inverted TATA element (nt -24 to -19), an inverted CCAAT box, a homeobox-binding site, a putative 1,25-dihydroxyvitamin D3 response element (VDRE) sequence overlapping the inverted TATA sequence, and a novel 18 nt palindrome that may control the tissue-specific transcription of the BSP gene. The shortest promoter sequence capable of directing bacterial chloramphenicol acetyltransferase reporter gene expression included the inverted TATA element and the inverted CCAAT box. However, the promoter activity was down-regulated by 1,25-dihydroxyvitamin D3, indicating that the unique VDRE-like sequence overlapping the TATA element is functional. Thus the rat BSP gene promoter is characterized by novel cis-acting elements that may be involved in hormone- and tissue-specific regulation of transcription.

The different effect on bone formation between 1,25-dihydroxyvitamin d3 and 24r,25-dihydroxyvitamin d3 administration on rat bone

Bone ◽  
1992 ◽  
Vol 13 (5) ◽  
pp. A24-A24
Author(s):  
Y. Nagai ◽  
T. Nakamura ◽  
H. Murayama ◽  
H. Yada ◽  
H. Yamato ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Rat Bone

scholarly journals Human dipeptidyl peptidase IV gene promoter: tissue-specific regulation from a TATA-less GC-rich sequence characteristic of a housekeeping gene promoter

1995 ◽  
Vol 311 (3) ◽  
pp. 835-843 ◽  
Author(s):  
S K Böhm ◽  
J R Gum ◽  
R H Erickson ◽  
J W Hicks ◽  
Y S Kim
Keyword(s):  
Hepg2 Cells ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Housekeeping Gene ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Flanking Sequence ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

The dipeptidyl peptidase IV gene encodes a plasma-membrane exopeptidase that is highly expressed in small intestine, lung and kidney. In order to better understand the mechanisms responsible for this tissue-specific expression we cloned, sequenced and functionally characterized the 5′-flanking region of the human dipeptidyl peptidase IV gene. The first 500 bases of the 5′-flanking sequence constituted an unmethylated CpG island, contained several Sp1-binding sites and lacked a consensus TATA box, all characteristics of gene promoters lacking tissue-specific expression. RNase-protection analysis using both small intestinal and Caco2 cell RNA indicated that the dipeptidyl peptidase IV transcript was initiated from no fewer than six major and 12 minor start sites. The 5′-flanking sequence also exhibited functional promoter activity in transient transfection experiments. Here, various lengths of the sequence were cloned upstream of a luciferase gene and introduced into cultured cells using lipofectin. A region located between bases -150 and -109 relative to the start of translation was found to be important for high-level promoter activity in both Caco2 and HepG2 cells. Moreover, Caco2 cells and HepG2 cells, which express high levels of dipeptidyl peptidase IV activity, exhibited much higher normalized luciferase activity after transfection than did 3T3, Jurkat or COS-7 cells, which have low enzyme levels. Sodium butyrate was found to increase both enzyme activity and normalized luciferase in HepG2 cells. Thus the dipeptidyl peptidase IV promoter possesses the ability to initiate transcription in a tissue-specific fashion in spite of having the sequence characteristics of a housekeeping gene promoter.

scholarly journals Tissue-specific expression from a compound TATA-dependent and TATA-independent promoter.

1990 ◽  
Vol 10 (11) ◽  
pp. 5646-5654 ◽  
Author(s):  
P A Garrity ◽  
B J Wold
Keyword(s):  
Cultured Cells ◽  
Core Promoter ◽  
Gene Promoter ◽  
Cell Types ◽  
Start Site ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tata Element ◽  
Different Types

We have found that the mouse metallothionein-I (MT-I) gene promoter functions in an unusual, compound manner. It directs both TATA-dependent and TATA-independent modes of transcription in vivo. The TATA-dependent message is initiated at the previously characterized +1 transcription start site and is the predominant species in most tissues. In many cell types it is metal inducible. The TATA-independent initiation sites are distributed over the 160 bp upstream of the previously characterized +1 start site, and the RNA products are present in all tissues examined. Only in testis, however, do the TATA-independent transcripts predominate, accumulating to highest levels in pachytene-stage meiotic cells and early spermatids. Unlike the TATA-dependent +1 transcript, these RNAs are not induced by metal, even in cultured cells in which the +1 species is induced. Transfection studies of site-directed mutants show that destruction of the TATA element drastically alters the ratio of the two RNA classes in cells in which the +1 transcripts normally dominates. In TATA-minus mutants, the TATA-independent RNAs become the most prevalent, although they remain refractory to metal induction. Thus, the MT-I promoter utilizes two different types of core promoter function within a single cell population. The two different types of core promoter respond very differently to environmental stimuli, and the choice between them appears to be regulated in a tissue-specific fashion.

Interaction of androgen and 1,25-dihydroxyvitamin D3: Effects on normal rat bone cells

2009 ◽  
Vol 7 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Colin Gray ◽  
Kay W. Colston ◽  
Alan G. Mackay ◽  
M. Louise Taylor ◽  
Timothy R. Arnett
Keyword(s):  
Bone Cells ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Normal Rat ◽  
Rat Bone
Sign in / Sign up

Export Citation Format

Share Document