Optical measurement of the plasma-membrane potential of mammalian cells grown in monolayer culture

1981 ◽  
Vol 9 (1) ◽  
pp. 80-81 ◽  
Author(s):  
C. LINDSAY BASHFORD ◽  
KEITH A. FOSTER ◽  
KINGSLEY J. MICKLEM ◽  
CHARLES A. PASTERNAK
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Mammalian Cells ◽  
Monolayer Culture ◽  
Optical Measurement ◽  
Plasma Membrane Potential

Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension

1985 ◽  
Vol 123 (3) ◽  
pp. 326-336 ◽  
Author(s):  
C. Lindsay Bashford ◽  
Glenn M. Alder ◽  
Michael A. Gray ◽  
Kingsley J. Micklem ◽  
C. Christopher Taylor ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Monolayer Culture ◽  
Animal Cells ◽  
Plasma Membrane Potential ◽  
Oxonol Dyes

scholarly journals TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential‐dependent coupling with PTEN

2019 ◽  
Vol 33 (9) ◽  
pp. 9785-9796 ◽  
Author(s):  
Takuro Numaga‐Tomita ◽  
Tsukasa Shimauchi ◽  
Sayaka Oda ◽  
Tomohiro Tanaka ◽  
Kazuhiro Nishiyama ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Membrane Potential ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Phenotypic Switching ◽  
Plasma Membrane Potential

scholarly journals Kinetic study of the plasma-membrane potential in procyclic and bloodstream forms of Trypanosoma brucei brucei using the fluorescent probe bisoxonol

1996 ◽  
Vol 314 (2) ◽  
pp. 595-601 ◽  
Author(s):  
Fabienne DEFRISE-QUERTAIN ◽  
Chantal FRASER-L'HOSTIS ◽  
Danièle CORAL ◽  
Jacques DESHUSSES
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Trypanosoma Brucei ◽  
Cultured Cells ◽  
Bloodstream Form ◽  
Metabolic Inhibitors ◽  
Trypanosoma Brucei Brucei ◽  
Plasma Membrane Potential ◽  
Regulation Mechanisms ◽  
K Concentration

The characteristics of the plasma-membrane potential of procyclic and bloodstream forms of Trypanosoma brucei brucei (cultured cells) were investigated using the fluorescent anionic probe bisoxonol. Observation of a stable and representative plasma-membrane potential in the resting state required careful washing, centrifugation and maintenance of the cells at room temperature before measurement. Bloodstream forms were more prone to depolarization during washing at 4 °C than procyclic cells. The higher fluorescence observed in the presence of long slender cells than in the presence of procyclic cells shows that the plasma-membrane potential is more negative in the insect form. Healthy dilute cells can sustain their plasma-membrane potential for hours in the presence of external glucose. The presence of a high K+ concentration in the medium did not promote by itself the depolarization of either type of cell. Study of bisoxonol fluorescence as a function of time allowed us to follow the kinetics of the action of metabolic inhibitors in the presence of various ions. o-Vanadate (1 mM) was found to depolarize bloodstream-form cells rapidly but only in a phosphate-free NaCl buffer. Omeprazole and strophanthidin also specifically depolarized bloodstream-form trypanosomes. However, NN´-dicyclohexylcarbodi-imide depolarized both types of cell, but more rapidly for bloodstream-form cells. Bloodstream-form trypanosomes appear to use mainly a vanadate-sensitive Na+ pump to maintain their Na+-diffusion gradient. However, most of the ATPase inhibitors tested had little or no effect on the plasma-membrane potential of procyclics suggesting that this form of trypanosome may rely on several regulation mechanisms.

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