Dispersed Human Anterior Pituitary Adenomas Preferentially Attach to Poly-L-Lysine Coated Wells

1991 ◽  
Vol 81 (s25) ◽  
pp. 29P-30P
Author(s):  
SL Atkin ◽  
AM Landolt ◽  
RV Jeffreys ◽  
White MC
Keyword(s):  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Human Anterior Pituitary

Interactions between normal and tumoral tissues at the boundary of human anterior pituitary adenomas

1994 ◽  
Vol 424 (1) ◽  
Author(s):  
M.R. Farnoud ◽  
F. Peillon ◽  
J.Y. Li ◽  
M. Kujas ◽  
J. Racadot ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Human Anterior Pituitary

scholarly journals 17β-Hydroxysteroid Dehydrogenase Type 1, 2, 3, and 4 Expression and Enzyme Activity in Human Anterior Pituitary Adenomas

1999 ◽  
Vol 84 (4) ◽  
pp. 1340-1345
Author(s):  
V. L. Green ◽  
V. Speirs ◽  
A. M. Landolt ◽  
P. M. Foy ◽  
S. L. Atkin
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Estrogenic Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Messenger Ribonucleic Acid ◽  
Human Anterior Pituitary

17β-Hydroxysteroid dehydrogenase (17βHSD) isoforms reversibly catalyze the final step in the formation of estradiol (E2) from estrone (E1) and the formation of testosterone from androstenedione. We have investigated 17βHSD type 1, 2, 3, and 4 gene expression and 17βHSD estrogenic activity in human anterior pituitary adenomas. 17βHSD messenger ribonucleic acid (mRNA) expression was studied by RT-PCR in 42 pituitary tumors and 3 normal pituitaries, 17βHSD activity was studied in 11 tumors and 17βHSD type 1 was immunolocalized in vitro in 6 tumors. 17βHSD type 1 gene expression was detected in 34 of 42 adenomas in all tumor subtypes; 17βHSD type 2 mRNA was detected in 18 of 42 adenomas, but not in prolactinomas; 17βHSD type 3 mRNA was detected in 12 of 42 adenomas, but not in corticotropinomas; 17βHSD type 4 was expressed in 20 of 42 adenomas by all adenoma subtypes. Reversible 17βHSD activity was found in 9 of 11 adenomas, and 17βHSD type 1 immunopositivity was cytoplasmically distributed in all 6 adenomas in vitro. All 4 17βHSD isoforms are variably expressed in human anterior pituitary adenomas, which also show 17βHSD enzyme activity, suggesting that 17βHSD may play an important role in regulating the local cellular levels of estradiol.

Expression of the transferrin receptor in human anterior pituitary adenomas is confined to gonadotrophinomas

1996 ◽  
Vol 44 (4) ◽  
pp. 467-471 ◽  
Author(s):  
S. L. Atkin ◽  
H. E. Burnett ◽  
V. L. Green ◽  
M. C. White ◽  
M. Lombard
Keyword(s):  
Transferrin Receptor ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Human Anterior Pituitary

Cytokine expression in human anterior pituitary adenomas

1996 ◽  
Vol 45 (2) ◽  
pp. 179-185 ◽  
Author(s):  
V. L. Green ◽  
S. L. Atkin ◽  
V. Speirs ◽  
R. V. Jeffreys ◽  
A. M. Landolt ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Cytokine Expression ◽  
Human Anterior Pituitary

scholarly journals Expression of 5'-deiodinase enzymes in normal pituitaries and in various human pituitary adenomas

2002 ◽  
pp. 263-268 ◽  
Author(s):  
A Baur ◽  
M Buchfelder ◽  
J Kohrle
Keyword(s):  
Thyroid Hormone ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Potent Inhibitor ◽  
Feedback Regulation ◽  
Cellular Distribution ◽  
Limited Information ◽  
Human Pituitary ◽  
Normal Tissues ◽  
Human Anterior Pituitary

OBJECTIVE: Local 5'-deiodination of l-thyroxine (T(4)) to active thyroid hormone 3,3',5-tri-iodothyronine (T(3)) catalyzed by the two 5'-deiodinase enzymes (D1 and D2) regulates various T(3)-dependent functions in the anterior pituitary and has been well studied in rodents. Only limited information about deiodinase expression and its cellular distribution in human anterior pituitaries is available. DESIGN: We examined 5'-deiodinase enzyme activities in pituitary adenomas (18 non-functioning, seven TSH-producing, one GH- and TSH-producing, five GH-producing, eight prolactin (PRL)-producing, two adenomas each from patients with Cushing's disease and Nelson's syndrome) and three normal anterior pituitaries. METHODS: Activities were measured as release of (125)I(-) from tyrosyl-ring labeled reverse T(3) with or without propylthiouracil, a potent inhibitor of D1 which does not influence D2 activities. RESULTS: Most of the adenomas and normal tissues expressed both isoenzymes, with D2 activity higher than D1. In a few tissues D1 activity was higher than D2 and some tissues did not express D1 activity at all. Highest activities of both enzymes were found in TSH- and PRL-producing adenomas but absolute activities and the D1/D2 ratio were variable in the same kind of tumor in different patients. CONCLUSION: The finding that all examined tissues expressed 5'-deiodinase activity, most of them expressing both isoenzymes, implies that both enzymes are still active in tumors and that local deiodination is important for the function and feedback regulation of human anterior pituitary.

A comparison of proliferation indices in human anterior pituitary adenomas using formalin-fixed tissue and in vitro cell culture

1997 ◽  
Vol 87 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Stephen L. Atkin ◽  
Victoria L. Green ◽  
Leslie J. Hipkin ◽  
Alex M. Landolt ◽  
Patrick M. Foy ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Labeling Index ◽  
Ki 67 ◽  
Histological Sections ◽  
Human Anterior Pituitary ◽  
Proliferative Indices

✓ The authors compared detection methods for cell proliferation in human anterior pituitary adenomas using histological sections and dispersed cell culture. After tumor cells had been grown for 4 days in dispersed culture, bromodeoxyuridine (BUdR), proliferating cell nuclear antigen (PCNA), and Ki-67 were compared by double immunostaining and contrasted with single staining of PCNA and Ki-67 indices in the corresponding histological sections from 12 human pituitary adenomas. In vitro, the BUdR labeling index was positive in six of 12 tumors (range < 0.1–5.1%), 10 of 12 tumors were PCNA-positive (range < 0.1–100%), and Ki-67 was positive in 10 of 12 adenomas (range < 0.1–8%). In vitro, BUdR and Ki-67 gave similar proliferative indices for 10 of 12 adenomas. In vivo, the PCNA labeling index was positive in 12 of 12 adenomas (range 0.9–95%) and Ki-67 was positive in 11 of 12 adenomas (range < 0.1–2%). Tumors with a labeling index less than 0.1% were considered to be negative for proliferation. High PCNA values were found in vitro and in vivo, whereas Ki-67 labeling indices were similar in vitro and in vivo for nine of 12 adenomas. It is concluded that Ki-67 proliferative indices in vivo reflect those found in vitro, at least after 4 days in dispersed culture, but that PCNA overestimates pituitary adenoma proliferation in histological sections as well as in dispersed culture.

Apoptosis and p53 suppressor gene protein expression in human anterior pituitary adenomas

1997 ◽  
Vol 136 (4) ◽  
pp. 382-387 ◽  
Author(s):  
Victoria L Green ◽  
Michael C White ◽  
Leslie J Hipkin ◽  
Richard V Jeffreys ◽  
Patrick M Foy ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
P53 Protein ◽  
Tumour Size ◽  
Suppressor Gene ◽  
Tumour Suppressor ◽  
Tumour Type ◽  
P53 Protein Expression ◽  
Human Anterior Pituitary

Abstract Human anterior pituitary adenomas proliferate and express the p53 tumour suppressor gene protein, but it is not known if apoptosis (programmed cell death) occurs. Therefore, the detection of apoptosis was undertaken in tumorous human anterior pituitary tissue and compared with p53 protein expression, tumour type and tumour size. Apoptosis (detected by the in situ end labelling technique) and p53 suppressor gene protein (detected by DO. 1-antibody immunocytochemistry) were determined in formalin-fixed and paraffin-embedded tissue from 37 human pituitary adenomas (2 macroprolactinomas, 9 somatotrophinomas and 26 non-functioning adenomas). Two normal anterior pituitaries were also included in this study. Pre-operative tumour size was scored 1 to 4 from magnetic resonance imaging radiology. Apoptosis was found in 7 of 29 tumours (24%), 11% of somatotrophinomas and 33% of non-functioning adenomas, although this difference was not significant. The p53 tumour suppressor protein was found in 7 of 31 tumours (23%), 33% of somatotrophinomas and 19% of nonfunctioning adenomas. Apoptosis and p53 protein expression were not found in normal anterior pituitary. In conclusion, apoptosis occurs in human anterior pituitary adenomas, but no significant association was found between apoptosis and p53 protein expression, tumour type or tumour size. European Journal of Endocrinology 136 382–387

Sign in / Sign up

Export Citation Format

Share Document