Genetic diversity of barley landrace accessions (Hordeum vulgare ssp. vulgare) conserved for different lengths of time in ex situ gene banks

Heredity ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 476-486 ◽  
Author(s):  
H K Parzies ◽  
W Spoor ◽  
R A Ennos
2017 ◽  
Vol 16 (2) ◽  
pp. 112-126 ◽  
Author(s):  
I. Thormann ◽  
P. Reeves ◽  
S. Thumm ◽  
A. Reilley ◽  
J. M. M. Engels ◽  
...  

AbstractIn many regions of the world, the cultivation of landraces is still common, in particular in centres of crop diversity. Significant effort has been put into ex situ conservation of landraces but limited data exist on the changes in genetic diversity that occur over time in farmers’ fields. We assessed temporal changes in barley landrace diversity in Jordan using seed samples collected in 1981 and 2012 from the same locations. We did not observe significant changes in the amount of genetic diversity, but samples collected in 2012 were more homogenous and less locally distinct. In two sites, we observed replacement of the old material. We observed a change in phenotype, and phenotypes were found to be more homogeneous among sites in 2012. Climate changed significantly over the study period, becoming hotter and dryer, but we did not identify any correlation between the changes in climate and genetic and phenotypic variations. While the amount of genetic diversity in terms of allelic richness and number of multi-locus genotypes has been maintained, local distinctiveness among landrace barley populations in Jordan was reduced.


2022 ◽  
Vol 12 ◽  
Author(s):  
Muluken Enyew ◽  
Tileye Feyissa ◽  
Anders S. Carlsson ◽  
Kassahun Tesfaye ◽  
Cecilia Hammenhag ◽  
...  

Ethiopia is the center of origin for sorghum [Sorghum bicolor (L.) Moench], where the distinct agro-ecological zones significantly contributed to the genetic diversity of the crops. A large number of sorghum landrace accessions have been conserved ex situ. Molecular characterization of this diverse germplasm can contribute to its efficient conservation and utilization in the breeding programs. This study aimed to investigate the genetic diversity of Ethiopian sorghum using gene-based single nucleotide polymorphism (SNP) markers. In total, 359 individuals representing 24 landrace accessions were genotyped using 3,001 SNP markers. The SNP markers had moderately high polymorphism information content (PIC = 0.24) and gene diversity (H = 0.29), on average. This study revealed 48 SNP loci that were significantly deviated from Hardy–Weinberg equilibrium with excess heterozygosity and 13 loci presumed to be under selection (P < 0.01). The analysis of molecular variance (AMOVA) determined that 35.5% of the total variation occurred within and 64.5% among the accessions. Similarly, significant differentiations were observed among geographic regions and peduncle shape-based groups. In the latter case, accessions with bent peduncles had higher genetic variation than those with erect peduncles. More alleles that are private were found in the eastern region than in the other regions of the country, suggesting a good in situ conservation status in the east. Cluster, principal coordinates (PCoA), and STRUCTURE analyses revealed distinct accession clusters. Hence, crossbreeding genotypes from different clusters and evaluating their progenies for desirable traits is advantageous. The exceptionally high heterozygosity observed in accession SB4 and SB21 from the western geographic region is an intriguing finding of this study, which merits further investigation.


2007 ◽  
Vol 58 (12) ◽  
pp. 1174 ◽  
Author(s):  
B. J. Stodart ◽  
M. C. Mackay ◽  
H. Raman

Diversity Arrays Technology (DArT™) was evaluated as a tool for determining molecular diversity of wheat landraces held within the Australian Winter Cereals Collection (AWCC). Initially, a set of 44 wheat landraces was evaluated with 256 DArT markers. The dataset was compared with the results obtained using 16 amplified fragment length polymorphism (AFLP) primer combinations and 63 simple sequence repeat (SSR) markers, mapped on the 21 chromosomes, from a previous study. The DArT markers exhibited a strong positive correlation with AFLP and SSR, with each marker type distinguishing similar relationships among the 44 landrace accessions. The DArT markers exhibited a higher polymorphic information content than AFLP, and were comparable with that obtained with SSR. Three hundred and fifty-five DArT markers were then used to evaluate genetic diversity among 705 wheat landrace accessions from within the AWCC, chosen to represent 5 world regions. DArT analysis was capable of distinguishing accessions from different geographic regions, and suggested that accessions originating from Nepal represent a unique gene pool within the collection. A statistical resampling of DArT loci indicated that 10–20 loci were enough to distinguish the maximum molecular diversity present within the collection. This research demonstrates the efficacy of the DArT platform as a tool for efficient examination of wheat diversity. As an ex situ germplasm repository, the AWCC contains wheat accessions of high genetic diversity, from genetically differentiated collection sites, even though diversity was under-represented in some countries represented in the repository.


1997 ◽  
Vol 95 (1-2) ◽  
pp. 199-204 ◽  
Author(s):  
A. H. del Rio ◽  
J. B. Bamberg ◽  
Z. Huaman ◽  
A. Salas ◽  
S. E. Vega

Hereditas ◽  
2002 ◽  
Vol 136 (1) ◽  
pp. 67-73 ◽  
Author(s):  
FANG LIU ◽  
GEN-LOU SUN ◽  
BJORN SALOMON ◽  
ROLAND VON BOTHMER

Diversity ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 315
Author(s):  
Martin N. A. Larsson ◽  
Matti W. Leino ◽  
Jenny Hagenblad

Landrace crops are important genetic resources, both for plant breeding efforts and for studying agrarian history. The distribution of genetic diversity among landraces can reflect effects of climate, economic structure, and trade also over a limited spatial and temporal scale. In this study, we have SNP genotyped historical barley seed samples from the late 19th century, together with extant barley landrace accessions from Jämtland, Sweden, a county centrally located, situated between Sweden and Norway. We found two main genetic clusters, one associated with the main agricultural district around lake Storsjön and one in the peripheral areas. Data was also compared with genotypes from landraces from across the Scandinavian peninsula. Accessions from the peripheral part of Jämtland show genetic similarity to accessions from a large part of central Scandinavia, while the accessions from the Storsjön district are more differentiabted. We suggest that these dissimilarities in genetic diversity distribution are explained by differences in the relative importance of agriculture and trading. We further compared the historical material with ex situ preserved extant landraces from the same region and found that their genetic diversity was not always representative of the given provenience. The historical material, in contrast, proved particularly valuable for assessing how crop genetic diversity has historically been influenced by economic focus.


Crop Science ◽  
2004 ◽  
Vol 44 (3) ◽  
pp. 997 ◽  
Author(s):  
A. A. Jaradat ◽  
M. Shahid ◽  
A. Al-Maskri

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