scholarly journals Role of seminal osmolarity in the regulation of human sperm motility

2002 ◽  
Vol 25 (4) ◽  
pp. 230-235 ◽  
Author(s):  
M. Rossato ◽  
G. Balercia ◽  
G. Lucarelli ◽  
C. Foresta ◽  
F. Mantero
Keyword(s):  
Sperm Motility ◽  
Human Sperm

Lysine glutarylation in human sperm is associated with progressive motility

2019 ◽  
Vol 34 (7) ◽  
pp. 1186-1194 ◽  
Author(s):  
Yi-min Cheng ◽  
Xiao-nian Hu ◽  
Zhen Peng ◽  
Ting-ting Pan ◽  
Fang Wang ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Natural Science ◽  
Hot Spot ◽  
Human Sperm ◽  
Mature Sperm ◽  
Computer Assisted ◽  
Structured Illumination Microscopy ◽  
Progressive Motility ◽  
Wide Range

AbstractSTUDY QUESTIONIs there a role for lysine glutarylation (Kglu), a newly identified protein post-translational modification (PTM), in human sperm?SUMMARY ANSWERKglu occurs in several proteins located in the tail of human sperm, and it was reduced in asthenozoospermic (A) men and positively correlated with progressive motility of human sperm, indicating its important role in maintaining sperm motility.WHAT IS KNOWN ALREADYSince mature sperm are almost transcriptionally silent, PTM is regarded as an important pathway in regulating sperm function. However, only phosphorylation has been extensively studied in mature sperm to date. Protein lysine modification (PLM), a hot spot of PTMs, was rarely studied except for a few reports on lysine methylation and acetylation. As a newly identified PLM, Kglu has not been well characterized, especially in mature sperm.STUDY DESIGN, SIZE, DURATIONSperm samples were obtained from normozoospermic (N) men and A men who visited the reproductive medical center between February 2016 and January 2018. In total, 61 N men and 59 A men were recruited to participate in the study.PARTICIPANTS/MATERIALS, SETTING, METHODSKglu was examined by immunoblotting and immunofluorescence assays using a previously qualified pan-anti-glutaryllysine antibody that recognizes glutaryllysine in a wide range of sequence contexts (both in histones and non-histone substrates) but not the structurally similar malonyllysine and succinyllysine. The immunofluorescence assay was imaged using laser scanning confocal microscopy and super-resolution structured illumination microscopy. Sperm motility parameters were examined by computer-assisted sperm analysis.MAIN RESULTS AND THE ROLE OF CHANCEKglu occurs in several proteins (20–150 kDa) located in the tail of human sperm, especially in the middle piece and the latter part of the principal piece. Sperm Kglu was modulated by regulatory systems (enzymes and glutaryl-CoA) similar to those in HeLa cells. The mean level of sperm Kglu was significantly reduced in A men compared with N men (P < 0.001) and was positively correlated with progressive motility (P < 0.001). The sodium glutarate-induced elevation of Kglu levels in A men with lower Kglu levels in sperm significantly improved the progressive motility (P < 0.001). Furthermore, the reduced sperm Kglu levels in A men was accompanied by an increase in sperm glutaryl-CoA dehydrogenase (a regulatory enzyme of Kglu).LARGE SCALE DATAN/ALIMITATIONS, REASONS FOR CAUTIONAlthough the present study indicated the involvement of sperm Kglu in maintaining progressive motility of human sperm, the underlying mechanism needs to be investigated further.WIDER IMPLICATIONS OF THE FINDINGSThe findings of this study provide an insight into the novel role of Kglu in human sperm and suggest that abnormality of sperm PLMs may be one of the causes of asthenozoospermia.STUDY FUNDING/COMPETING INTEREST(S)National Natural Science Foundation of China (81 771 644 to T.L.; 31 671 204 to X.Z. and 81 871 207 to H.C.); National Basic Research Program of China (973 Program, 2015CB943003 to X.Z.); Natural Science Foundation of Jiangxi, China (20171ACB21006 and 20161BAB204167 to T.L.; 20165BCB18001 to X.Z.). The authors have no conflicts of interest to declare.

scholarly journals The role of Na,K-ATPase in human sperm motility

2002 ◽  
Vol 25 (3) ◽  
pp. 180-185 ◽  
Author(s):  
N. Kocak-toker ◽  
G. Aktan ◽  
G. Aykac-toker
Keyword(s):  
Sperm Motility ◽  
Human Sperm

scholarly journals Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Hwang I. S. Thomas ◽  
Ying-Shiuan Chen ◽  
Ching-Han Hung ◽  
Dilip Bhargava Sreerangaraja Urs ◽  
Tien-Ling Liao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Sperm Motility ◽  
Manganese Superoxide Dismutase ◽  
Uncoupling Protein ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Oxygen Species ◽  
Genotype Frequencies ◽  
The Impact

Sperm motility is one of the major determinants of male fertility. Since sperm need a great deal of energy to support their fast movement by active metabolism, they are thus extremely vulnerable to oxidative damage by the reactive oxygen species (ROS) and other free radicals generated as byproducts in the electron transport chain. The present study is aimed at understanding the impact of a mitochondrial oxidizing/reducing microenvironment in the etiopathology of male infertility. We detected the mitochondrial DNA (mtDNA) 4,977 bp deletion in human sperm. We examined the gene mutation of ATP synthase 6 (ATPase6 m.T8993G) in ATP generation, the gene polymorphisms of uncoupling protein 2 (UCP2, G-866A) in the uncoupling of oxidative phosphorylation, the role of genes such as manganese superoxide dismutase (MnSOD, C47T) and catalase (CAT, C-262T) in the scavenging system in neutralizing reactive oxygen species, and the role of human 8-oxoguanine DNA glycosylase (hOGG1, C1245G) in 8-hydroxy-2 ′ -deoxyguanosine (8-OHdG) repair. We found that the sperm with higher motility were found to have a higher mitochondrial membrane potential and mitochondrial bioenergetics. The genotype frequencies of UCP2 G-866A, MnSOD C47T, and CAT C-262T were found to be significantly different among the fertile subjects, the infertile subjects with more than 50% motility, and the infertile subjects with less than 50% motility. A higher prevalence of the mtDNA 4,977 bp deletion was found in the subjects with impaired sperm motility and fertility. Furthermore, we found that there were significant differences between the occurrences of the mtDNA 4,977 bp deletion and MnSOD (C47T) and hOGG1 (C1245G). In conclusion, the maintenance of the mitochondrial redox microenvironment and genome integrity is an important issue in sperm motility and fertility.

scholarly journals Energetic Metabolism and Human Sperm Motility: Impact of CB1 Receptor Activation

Endocrinology ◽  
2010 ◽  
Vol 151 (12) ◽  
pp. 5882-5892 ◽  
Author(s):  
A. Barbonetti ◽  
M. R. C. Vassallo ◽  
D. Fortunato ◽  
S. Francavilla ◽  
M. Maccarrone ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Sperm Motility ◽  
Cannabinoid Receptor ◽  
Human Sperm ◽  
Receptor Activation ◽  
Mitochondrial Activity ◽  
Energetic Metabolism ◽  
Cb1 Cannabinoid Receptor ◽  
High Doses

It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role of AEA-binding receptors in mediating mitochondrial inhibition has not yet been explored. In this study, human sperm exposure to Met-AEA (methanandamide, nonhydrolyzable analog of AEA) in the micromolar range significantly decreased mitochondrial transmembrane potential (ΔΨm), similarly to rotenone, mitochondrial complex I inhibitor. The effect of Met-AEA (1 μm) was prevented by SR141716, CB1 cannabinoid receptor antagonist, but not by SR144528, CB2 antagonist, nor by iodoresiniferatoxin, vanilloid receptor antagonist. The effect of Met-AEA did not involve activation of caspase-9 or caspase-3 and was reverted by washing. In the presence of glucose, sperm exposure either to Met-AEA up to 1 μm or to rotenone for up to 18 h did not affect sperm motility. At higher doses Met-AEA produced a CB1-independent poisoning of spermatozoa, reducing their viability. Under glycolysis blockage, 1 μm Met-AEA, similarly to rotenone, dramatically abolished sperm motility, an effect that was prevented by SR1 and reverted by washing. In conclusion, CB1 activation induced a nonapoptotic decrease of ΔΨm, the detrimental reflection on sperm motility of which could be revealed only under glycolysis blockage, unless very high doses of Met-AEA, producing CB1-independent sperm toxicity, were used. The effects of CB1 activation reported here contribute to elucidate the relationship between energetic metabolism and human sperm motility.

scholarly journals Role of Nitric Oxide Concentrations on Human Sperm Motility

2004 ◽  
Vol 25 (2) ◽  
pp. 245-249 ◽  
Author(s):  
Giancarlo Balercia ◽  
Simona Moretti ◽  
Arianna Vignini ◽  
Mattia Magagnini ◽  
Franco Mantero ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Sperm Motility ◽  
Human Sperm

Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation

2006 ◽  
Vol 63 (2) ◽  
pp. 66-76 ◽  
Author(s):  
Zoltán Krasznai ◽  
Zoárd Tibor Krasznai ◽  
Masaaki Morisawa ◽  
Zsuzsa Kassai Bazsáné ◽  
Zoltán Hernádi ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Calcium Homeostasis ◽  
Human Sperm ◽  
Motility Regulation

scholarly journals The role of mitochondria in energy production for human sperm motility

2011 ◽  
Vol 35 (2) ◽  
pp. 109-124 ◽  
Author(s):  
P. Piomboni ◽  
R. Focarelli ◽  
A. Stendardi ◽  
A. Ferramosca ◽  
V. Zara
Keyword(s):  
Sperm Motility ◽  
Energy Production ◽  
Human Sperm

scholarly journals Role of Angiotensin-(1–7) via MAS receptor in human sperm motility and acrosome reaction

Reproduction ◽  
2020 ◽  
Vol 159 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Asier Valdivia ◽  
Lorea Cortés ◽  
Maider Beitia ◽  
Lide Totorikaguena ◽  
Naiara Agirregoitia ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Acrosome Reaction ◽  
Mrna Level ◽  
Human Sperm ◽  
Human Spermatozoa ◽  
Dependent Manner ◽  
Mas Receptor ◽  
Sperm Mobility ◽  
Specific Antagonist

Rennin-angiotensin system (RAS) has been involved in sperm function, even so, little is known about the implication of one of the RAS axis formed by Ang-(1–7) (angiotensin-(1–7)) and MAS receptor. Hence, in the present work, we focused on elucidating the function of the MAS receptor in human spermatozoa. We analyzed the expression and localization of MAS receptor in human spermatozoa and we observed if its activation is able to modulate the sperm motility of normal motility and/or asthenozoospermic patients, as well as, the acrosome reaction of the spermatozoa. MAS receptor is present in human mature spermatozoa, not only at the mRNA level but also at protein level. MAS is localized at the acrosome region, as well as, in the tail of spermatozoa. The sperm incubation with MAS agonist Ang-(1–7) activates at dose-dependent manner the PI3K/AKT pathway (P < 0.01 vs control) and improves the motility of asthenozoospermic patients (P < 0.01 vs control), which is blocked by the specific antagonist (A779) (P < 0.01), but it do not modulate the acrosome reaction. These findings suggest that the ACE2/Ang-(1–7)/Mas axis may be a useful biochemical tool for the treatment of male infertility related to sperm mobility.

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