IFN‐γ Restores HIV‐ and Non‐HIV‐Specific Cell Mediated Immune Response
            In Vitro
            and Its Activity is Neutralized by Antibodies from Patients with AIDS

Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.

Download Full-text

CELL-MEDIATED IMMUNE RESPONSE IN VITRO

Journal of Experimental Medicine ◽

10.1084/jem.136.2.331 ◽

1972 ◽

Vol 136 (2) ◽

pp. 331-343 ◽

Cited By ~ 124

Author(s):

Hermann Wagner ◽

Marc Feldmann ◽

W. Boyle ◽

J. W. Schrader

Keyword(s):

Immune Response ◽

Peritoneal Macrophages ◽

Lymphoid Cells ◽

Cytotoxic Lymphocytes ◽

Separation Procedure ◽

Macrophage Depletion ◽

Cell Mediated Immune Response ◽

Immune Response In Vitro ◽

Allograft Response

All efficient cell separation procedure and specific anti-macrophage serum were used to investigate the requirement of macrophages in the in vitro allograft response of mouse lymphoid cells. The efficiency of the macrophage-depletion procedure used and the undiminished capacity of the purified lymphocytes to respond were verified by also testing the antibody responses to sheep red cells (SRC) and dinitrophenylated polymeric flagellin (DNP POL) as well as the proliferative response to allogeneic cells. It was found that the generation of cytotoxic lymphocytes were diminished after macrophage depletion by surface adherence. The combination of anti-macrophage serum and column purification resulted in the total abolition of cytotoxic activity. The cell-mediated immune response was restored completely by addition of peritoneal macrophages, with as few as 1 macrophage to 600 lymphocytes permitting a significant restoration. Macrophages were not involved in the cytotoxic effector phase, but were essential in immune induction. A subcellular H-2 alloantigen preparation was only immunogenic in the presence of macrophages, indicating that a mere reduction in the size of the antigen from cell-bound alloantigens to membrane fragments was not the sole function of macrophages. The results suggest that macrophages collaborate with T cells in the initiation of an allograft response in vitro.

Download Full-text

Cell-mediated immune response in vitro

Cellular Immunology ◽

10.1016/0008-8749(72)90004-4 ◽

1972 ◽

Vol 4 (1) ◽

pp. 39-50 ◽

Cited By ~ 78

Author(s):

Hermann Wagner ◽

Alan W. Harris ◽

Marc Feldmann

Keyword(s):

Immune Response ◽

Cell Mediated Immune Response ◽

Immune Response In Vitro

Download Full-text