scholarly journals Functional characteristics of two BKCa channel variants differentially expressed in rat brain tissues

2000 ◽  
Vol 267 (3) ◽  
pp. 910-918 ◽  
Author(s):  
Tal Soo Ha ◽  
Soon Youn Jeong ◽  
Suk-Woo Cho ◽  
Hyun-kyu Jeon ◽  
Gu Seob Roh ◽  
...  
Keyword(s):  
Rat Brain ◽  
Differentially Expressed ◽  
Bkca Channel ◽  
Functional Characteristics ◽  
Brain Tissues

scholarly journals Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Litao Li ◽  
Lipeng Dong ◽  
Jingru Zhao ◽  
Weiliang He ◽  
Bao Chu ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Rat Brain ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Blood Samples ◽  
Rat Blood ◽  
Target Mrnas ◽  
Potential Biomarker ◽  
Differentially Expressed Mirnas ◽  
Brain Tissues

Objective. With the growing incidence of ischemic stroke worldwide, there is an urgent need to identify blood biomarkers for ischemic stroke patients. Thus, our aim was to identify potential circulating microRNA (miRNA) as a potential biomarker and to explore its potential mechanism for ischemic stroke in rats. Methods. The mRNA dataset GSE97537 and miRNA dataset GSE97532 were downloaded from the Gene Expression Omnibus (GEO) GSE97537 including 7 middle cerebral artery occlusion (MCAO) rat brain tissues and 5 sham-operated rat brain tissues GSE97532 including 6 MCAO rat blood samples and 3 sham-operated rat blood samples. Differentially expressed mRNAs and miRNAs with corrected p value ≤ 0.01 and fold change ≥2 or ≤0.05 were identified. To explore potential biological processes and pathways of differentially expressed mRNAs, functional enrichment analysis was performed. The target mRNAs of differentially expressed miRNAs were predicted using DNA Intelligent Analysis (DIANA)-microT tools. The target mRNAs and differentially expressed mRNAs were intersected. Results. 1228 differentially expressed mRNAs in MCAO rat brain tissues were identified. Highly expressed mRNAs were mainly enriched in the inflammatory responses. Nine differentially expressed miRNAs were identified in MCAO rat blood samples. A total of 673 target mRNAs were predicted to significantly bind these differentially expressed miRNAs. Among them, 54 target mRNAs were differentially expressed in MCAO rat blood samples. Enrichment analysis results showed that these 54 target mRNAs were closely related to neurological diseases and immune responses. Among all miRNA-mRNA relationship, miR-3552-CASP3 interaction was identified, indicating that CASP3 might be mediated by miR-3552. Functional enrichment analysis revealed that CASP3 was involved in the apoptosis pathway, indicating that miR-3552 might participate in apoptosis by CASP3. Conclusion. Our findings reveal that circulating miR-3552 shows promise as a potential biomarker for ischemic stroke in rats.

Nerve growth factor (NGF) content in adult rat brain tissues is several-fold higher than generally reported and is largely associated with sedimentable fractions

1996 ◽  
Vol 728 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Marius C. Hoener ◽  
Eleanore Hewitt ◽  
James M. Conner ◽  
James W. Costello ◽  
Silvio Varon
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Nerve Growth ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Brain Tissues

Determination of Protopine in Rat Brain Tissues by RRLC-ESI/Q-TOF-MS Method

2014 ◽  
Vol 6 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Pei-xiang Wang ◽  
Yong-hui Li ◽  
Yan-jing Li ◽  
Ting Geng ◽  
Ming-li Li ◽  
...  
Keyword(s):  
Rat Brain ◽  
Tof Ms ◽  
Brain Tissues

scholarly journals Proteomics Analysis Reveals the Implications of Cytoskeleton and Mitochondria in the Response of the Rat Brain to Starvation

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 219 ◽  
Author(s):  
Beatriz Cuevas-Fernández ◽  
Carlos Fuentes-Almagro ◽  
Juan Peragón
Keyword(s):  
Mass Spectrometry ◽  
Rat Brain ◽  
Food Deprivation ◽  
Metabolic Response ◽  
Differentially Expressed ◽  
Nervous Impulse ◽  
Altered Expression ◽  
Peptide Mass ◽  
The Brain

Long-term starvation provokes a metabolic response in the brain to adapt to the lack of nutrient intake and to maintain the physiology of this organ. Here, we study the changes in the global proteomic profile of the rat brain after a seven-day period of food deprivation, to further our understanding of the biochemical and cellular mechanisms underlying the situations without food. We have used two-dimensional electrophoresis followed by mass spectrometry (2D-MS) in order to identify proteins differentially expressed during prolonged food deprivation. After the comparison of the protein profiles, 22 brain proteins were found with altered expression. Analysis by peptide mass fingerprinting and MS/MS (matrix-assisted laser desorption-ionization-time of flight mass spectrometer, MALDI-TOF/TOF) enabled the identification of 14 proteins differentially expressed that were divided into 3 categories: (1) energy catabolism and mitochondrial proteins; (2) chaperone proteins; and (3) cytoskeleton, exocytosis, and calcium. Changes in the expression of six proteins, identified by the 2D-MS proteomics procedure, were corroborated by a nanoliquid chromatography-mass spectrometry proteomics procedure (nLC-MS). Our results show that long-term starvation compromises essential functions of the brain related with energetic metabolism, synapsis, and the transmission of nervous impulse.

Improved approach for comprehensive profiling of gangliosides and sulfatides in rat brain tissues by using UHPLC-Q-TOF-MS

2019 ◽  
Vol 225 ◽  
pp. 104813
Author(s):  
Xiong-Yu Meng ◽  
Lee-Fong Yau ◽  
Hao Huang ◽  
Wai-Him Chan ◽  
Pei Luo ◽  
...  
Keyword(s):  
Rat Brain ◽  
Tof Ms ◽  
Brain Tissues
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