Changes in Oxygenation States of Rat Brain Tissues during Glutamate-Related Epileptic Seizures - Near-Infrared Study

Rodents are popular biological models for physiological and behavioral research in neuroscience and rats are better models than mice due to their higher genome similarity to human and more accessible surgical procedures. However, rat brain is larger than mice brain and it needs powerful imaging tools to implement better penetration against the scattering of the thicker brain tissue. Three-photon fluorescence microscopy (3PFM) combined with near-infrared (NIR) excitation has great potentials for brain circuits imaging because of its abilities of anti-scattering, deep-tissue imaging, and high signal-to-noise ratio (SNR). In this work, a type of AIE luminogen with red fluorescence was synthesized and encapsulated with Pluronic F-127 to make up form nanoparticles (NPs). Bright DCDPP-2TPA NPs were employed for in vivo three-photon fluorescent laser scanning microscopy of blood vessels in rats brain under 1550[Formula: see text]nm femtosecond laser excitation. A fine three-dimensional (3D) reconstruction up to the deepness of 600[Formula: see text][Formula: see text]m was achieved and the blood flow velocity of a selected vessel was measured in vivo as well. Our 3PFM deep brain imaging method simultaneously recorded the morphology and function of the brain blood vessels in vivo in the rat model. Using this angiography combined with the arsenal of rodent’s brain disease, models can accelerate the neuroscience research and clinical diagnosis of brain disease in the future.

Download Full-text

Nerve growth factor (NGF) content in adult rat brain tissues is several-fold higher than generally reported and is largely associated with sedimentable fractions

Brain Research ◽

10.1016/0006-8993(96)00386-1 ◽

1996 ◽

Vol 728 (1) ◽

pp. 47-56 ◽

Cited By ~ 27

Author(s):

Marius C. Hoener ◽

Eleanore Hewitt ◽

James M. Conner ◽

James W. Costello ◽

Silvio Varon

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Rat Brain ◽

Nerve Growth ◽

Adult Rat ◽

Adult Rat Brain ◽

Brain Tissues

Download Full-text

Plucking a hydrogen bond: A near infrared study of all four intermolecular modes in (DF)2

The Journal of Chemical Physics ◽

10.1063/1.471978 ◽

1996 ◽

Vol 105 (16) ◽

pp. 6645-6664 ◽

Cited By ~ 21

Author(s):

Scott Davis ◽

David T. Anderson ◽

David J. Nesbitt

Keyword(s):

Hydrogen Bond ◽

Near Infrared ◽

Infrared Study

Download Full-text

Optical properties of selected native and coagulated human brain tissues in vitro in the visible and near infrared spectral range

Physics in Medicine and Biology ◽

10.1088/0031-9155/47/12/305 ◽

2002 ◽

Vol 47 (12) ◽

pp. 2059-2073 ◽

Cited By ~ 341

Author(s):

A N Yaroslavsky ◽

P C Schulze ◽

I V Yaroslavsky ◽

R Schober ◽

F Ulrich ◽

...

Keyword(s):

Optical Properties ◽

Human Brain ◽

Spectral Range ◽

Near Infrared ◽

Infrared Spectral Range ◽

Infrared Spectral ◽

Brain Tissues

Download Full-text

Water Properties of Soft Contact Lenses: A Comparative Near-Infrared Study of Two Hydrogel Materials

International Journal of Polymer Science ◽

10.1155/2016/3737916 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Jelena Munćan ◽

Ivana Mileusnić ◽

Jovana Šakota Rosić ◽

Aleksandra Vasić-Milovanović ◽

Lidija Matija

Keyword(s):

Water Content ◽

Near Infrared ◽

Contact Lenses ◽

Polymer Network ◽

The State ◽

Vinyl Pyrrolidone ◽

Soft Contact ◽

Infrared Study ◽

Soft Contact Lenses ◽

State Of Water

The functionality of soft contact lenses depends strongly on the water content and their water-transport ability. This study was conducted in order to examine the state of water in two sets of soft contact lenses: VSO38, pHEMA Filcon I 1, and VSO50, copolymer of HEMA and VP Filcon II 1 (HEMA = 2-hydroxy-ethyl methacrylate; VP = vinyl pyrrolidone). Hydrogel lenses were studied using near-infrared spectroscopy and the novel Aquaphotomics approach in order to determine the state of water in materials based on their near-infrared spectra. Aquaphotomics approach investigates absorption at specific vibrational bands of water’s covalent and hydrogen bonds which can provide information on how the water structure changes with the structural change of the polymer network. Principal component analysis and specific star-chart “aquagram” were used to analyse water spectral pattern in hydrogel materials. The findings show that material VSO38 has water predominantly organized in bound state, while material with higher water content, VSO50, has more free and weakly hydrogen bonded water. Our findings define in detail exact water species existing and interacting with the polymer network. The results show qualitative and quantitative possibilities of Aquaphotomics for better modelling and understanding water behaviour in hydrogel materials.

Download Full-text