Marker-assisted selection of common beans for resistance to common bacterial blight: efficacy and economics

2000 ◽  
Vol 119 (5) ◽  
pp. 411-415 ◽  
Author(s):  
K. Yu ◽  
S.J. Park ◽  
V. Poysa
Keyword(s):  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Common Beans ◽  
Selection Of

scholarly journals Use of Marker-assisted Selection to Breed for Resistance to Common Bacterial Blight in Common Bean

2007 ◽  
Vol 132 (3) ◽  
pp. 381-386 ◽  
Author(s):  
Patrick D. O'Boyle ◽  
James D. Kelly ◽  
William W. Kirk
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Field Experiments ◽  
Selection Process ◽  
Bacterial Pathogen ◽  
Common Bacterial Blight ◽  
Epistatic Interactions ◽  
Sequence Characterized Amplified Region ◽  
Selection Of

Common bacterial blight (CBB), incited by the bacterial pathogen Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin et al., is one of the most serious seed-borne diseases of common bean (Phaseolus vulgaris L.) worldwide. Although previous attempts to breed CBB-resistant bean cultivars have had limited success due to the quantitative nature of resistance, progress in marker-assisted selection has created new opportunities for breeders to improve the selection process. The potential of independent linked markers for indirect selection of CBB resistance was evaluated in field experiments in East Lansing and Saginaw, Mich. The presence of the SU91 marker was correlated with lower CBB leaf scores in East Lansing (r = −0.50***) and Saginaw (r = −0.59***) and correlated with pod resistance in Saginaw (r = −0.48***). SU91 exhibited a slight negative correlation with yield (r = −0.20*) in East Lansing but showed no association with yield in Saginaw. Plant selections carrying SU91 were crossed with a different source of CBB resistance linked to the sequence-characterized amplified region marker BC420 on bean linkage group B6. The effects of SU91 and BC420 markers were examined in two greenhouse studies. The presence of SU91 was correlated with lower CBB disease ratings for leaves (r = −0.20*) and pods (r = −0.27***). Presence of BC420 was only correlated with low pod ratings (r = −0.19*) in one experiment and CBB leaf resistance (r = −0.18*) in the second experiment. Presence of both markers resulted in lower levels of CBB resistance than provided by either marker alone, possibly indicating epistatic interactions between the independent loci conditioning CBB resistance in common bean.

Direct and Marker-Assisted Selection for Resistance to Common Bacterial Blight in Common Bean

Crop Science ◽  
2012 ◽  
Vol 52 (4) ◽  
pp. 1511-1521 ◽  
Author(s):  
Robert W. Duncan ◽  
Robert L. Gilbertson ◽  
Shree P. Singh
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Selection For

scholarly journals Detection and quantification of Xanthomonas axonopodis pv. phaseoli and its variant fuscans in common bean seeds

2013 ◽  
Vol 35 (4) ◽  
pp. 428-434 ◽  
Author(s):  
Norimar D'Ávila Denardin ◽  
Vanessa Andréia Agostini
Keyword(s):  
Bacterial Blight ◽  
Seed Quality ◽  
Culture Media ◽  
Common Bacterial Blight ◽  
Common Beans ◽  
Xanthomonas Axonopodis ◽  
Overnight Incubation ◽  
Efficient Detection ◽  
The Common

The common bacterial blight of common beans (CBCF), a disease caused by Xanthomonas axonopodis pv. phaseoli (Xap) and Xanthomonas axonopodis pv. phaseoli var. fuscans (Xapf), significantly reduces grain yield and seed quality. Because this bacterium is mainly disseminated through infected seeds, efficient detection of Xap and Xapf is important to assure the productivity and quality of the crop. In this study, various techniques that included different extraction techniques (two different incubation times, with and without centrifugation) and five culture media (Kado 523, GYCA, MXP, NSA, and PTSA) were tested for the detection of the seed-borne inoculum, using three different seed samples. Overnight incubation of the seeds, followed by centrifugation and incubation in Kado 523 resulted in higher extraction of Xap and Xapf. The best extraction technique was overnight incubation followed by centrifugation, and the best medium was PTSA. Among the tested culture media, PTSA provided better identification and counting of the bacterial colonies, thus allowing the quantification of the seed infection levels.

Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight

2015 ◽  
Vol 184 ◽  
pp. 1-8 ◽  
Author(s):  
Dahu Ni ◽  
Fengshun Song ◽  
Jinlong Ni ◽  
Aifang Zhang ◽  
Chunlian Wang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Marker Assisted Selection ◽  
Rice Blast ◽  
Bacterial Blight ◽  
Hybrid Rice ◽  
Selection Of

scholarly journals Inheritance of Resistance to Common Bacterial Blight in Tepary Beans

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 839F-840
Author(s):  
A. Dursun ◽  
D.P. Coyne ◽  
M.F. Mohamed ◽  
G. Jung
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Transgressive Segregation ◽  
Inheritance Of Resistance ◽  
Common Bacterial Blight ◽  
Common Beans ◽  
Serious Disease ◽  
Different Strains ◽  
Dominant Genes

Common bacterial blight, incited by the bacterium Xanthomonas campestris pv. phaseoli (Xcp), is a serious disease of common beans [Phaseolus vulgaris (P. v.)]. Some tepary beans (P. acutifolius) are resistant (R) to Xcp and used to breed P. v. with R to Xcp. The objective was to determine the inheritance of the reaction to different strains of Xcp in crosses between susceptible (S) and R tepary lines. The parents, F2, and F3 populations from six tepary crosses involving 3 R × S, 1 R × moderately (M) R, and 2 R × R were inoculated with Xcp strains EK-11, LB-2, and SC-4A. Different single dominant genes controlled the reaction to different Xcp isolates in R × S crosses. Coupling linkage was detected between the genes controlling the reactions to each of the Xcp strains in the crosses NE #4B(s) × NE #19(R) and NE #4B(S) × CIAT-640005(R), except for NE #8A(MR) × NE #4B(S) with strains EK-11 and LB-2 and EK-11 and SC-4A. Transgressive segregation for S was observed in the F2 and F3 NE #8A × NE #8B(R), indicating that the parents possessed different genes for R. No segregation for reactions occurred n the F2 NE #8B × NE #19 and NE #19 × CIAT-640005, indicating that these parents possessed the same genes for R to the three strains.

scholarly journals Marker assisted selection of xa5, xa13 and Xa21 gene in breeding populations derived from Karma Mahsuri x IRBB 59

2021 ◽  
Vol 4 (1) ◽  
pp. 108-116
Author(s):  
A. J. Kotasthane ◽  
N. J. Gaikwad
Keyword(s):  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Durable Resistance ◽  
Gene Pyramiding ◽  
Effective Means ◽  
Rice Cultivar ◽  
Breeding Populations ◽  
Broad Spectrum Resistance ◽  
Serious Disease ◽  
Selection Of

Bacterial leaf blight, caused by the Gram negative bacterium Xanthomonas oryzae pv. oryzae (Xoo), is a serious disease throughout the rice growing world. Resistant cultivars are the primary and most effective means of control. Marker assisted selection (MAS) can help in screening more efficiently for the presence or absence of resistant genes. Molecular markers have made it possible to identify and pyramid valuable genes of agronomic importance in resistance rice breeding. In the present study, to incorporate durable resistance against bacterial blight three resistance genes, xa 5, xa13 and Xa21, from an indica donor IRBB 59 were introgressed into high yielding susceptible rice cultivar Karma Mahsuri. Karma Mahsuri is one of the most popular varieties of Chhattisgarh and mega varieties of India. These three genes were pyramided through marker-assisted breeding. For MAS of xa5:- RG556, RM122, RM390, RM13;  xa13:-RG136 and RM 230 and  Xa21: Xa21 and RM21 are the known linked markers. Markers xa5R and xa5S specific for xa5 resistant and susceptible genes respectively, xa13Pro for xa13 gene and PT248 for Xa21 gene obtained from Dr Sundaram (DRR, Hyderabad) were also used in the present study for MAS. High-resolution maps generated in silico around xa5 and xa13 will be useful for the precise placement of a gene of interest and the analysis of regional and sub-regional rates of recombination and appropriate combinations of markers for marker assisted selection in plant-breeding. In Karma Mahsuri X IRBB 59 cross we got Three lines (03)containing three gene (xa5, xa13 and Xa21), Twenty three (23) line contain a combination of xa5 & xa13,  only one (01) with xa5 and Xa21. There were eight lines with xa5 gene Seventeen (17) lines with xa13 gene. We therefore report herein the development of nil, two and three gene pyramids of  xa5, xa13 and Xa21 in the background of Karma Mahsuri. Key words: bacterial blight (BB), Broad-spectrum resistance, Gene pyramiding marker-assisted selection (MAS), Rice.

scholarly journals Photoperiod Sometimes Influences Common Bacterial Blight Disease of Common Beans

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 551-553 ◽  
Author(s):  
Mohamed F. Mohamed ◽  
Dermot P. Coyne
Keyword(s):  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Hypersensitive Reaction ◽  
Short Photoperiod ◽  
Post Inoculation ◽  
Common Bacterial Blight ◽  
Common Beans ◽  
Flower Bud ◽  
Pod Development ◽  
High Level

Common bacterial blight, incited by Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp), is a serious disease of common beans (Phaseolus vulgaris L.). Three experiments were conducted twice in growth chambers at 26 ± 1C under short (10 hours light/14 hours dark) and long (16 hours light/8 hours dark) photoperiods to determine the influence of these photoperiods, flower bud removal, pod development, and pre- and post-inoculation photoperiods on the reaction of common beans to Xcp. In one test, `PC-50' (susceptible; S) flowered earlier and was more susceptible to Xcp under the short photoperiod than under the long photoperiod. BAC-6 (resistant; R) flowered at the same time under both photoperiods but developed rapid leaf chlorosis (RLC) (hypersensitive reaction) under long photoperiods. Flowering and disease reactions to Xcp by XAN-159 (R) were similar under both photoperiods. In a second test, daily removal of flower buds of `PC-50' decreased its susceptibility to Xcp under the short photoperiod. RLC of inoculated leaves of BAC-6 occurred during flowering and pod development under both photoperiods. XAN-159 expressed a high level of resistance to Xcp but showed RLC at later pod development stages. In a third test, the disease reaction of `PC-50' was affected by the particular photoperiod applied post-inoculation but was not influenced by the photoperiod applied before inoculation with Xcp. The implications of these results in breeding beans for resistance to Xcp are discussed.

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