scholarly journals Evaluation of Affymetrix Gene Chip sensitivity in rat hippocampal tissue using SAGE analysis*

2002 ◽  
Vol 16 (3) ◽  
pp. 409-413 ◽  
Author(s):  
Simon J. Evans ◽  
Nicole A. Datson ◽  
Mohamed Kabbaj ◽  
Robert C. Thompson ◽  
Erno Vreugdenhil ◽  
...  
Keyword(s):  
Gene Chip ◽  
Hippocampal Tissue ◽  
Affymetrix Gene Chip

03 Affymetrix gene chip analysis of differentially expressed genes in rat post MI CHF model

2002 ◽  
Vol 34 (7) ◽  
pp. A11 ◽  
Author(s):  
Jian Wang ◽  
Lawrence Gelbert ◽  
Chad D. Geringer ◽  
Todd ◽  
G. Cook ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Gene Chip ◽  
Differentially Expressed ◽  
Affymetrix Gene Chip ◽  
Chip Analysis

Pre-Processing of Affymetrix Gene Chip Microarray Data

2010 ◽  
Vol 5 (4) ◽  
pp. 270-279
Author(s):  
Ahmed R. Hasan ◽  
John E. Pattison ◽  
Alex Hariz
Keyword(s):  
Microarray Data ◽  
Gene Chip ◽  
Affymetrix Gene Chip

scholarly journals Análise diferencial de genes em linhagens de células de leucemia

2020 ◽  
Vol 16 (6) ◽  
Author(s):  
Adilis Rodrigues da Silva ◽  
Andressa De Solza Fernandes ◽  
Viviane Paraboni Fiorin ◽  
Tuane Zeppenfeld ◽  
Guilherme Brum França ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Omnibus ◽  
Gene Chip ◽  
Terapia Génica ◽  
Leucemia Mieloide Crónica ◽  
Affymetrix Gene Chip

O câncer manifesta-se a partir de uma transmutação genética, ou seja, uma modificação no DNA que passa a transmitir informações erradas para o desenvolvimento de suas atividades, ocorrendo assim em genes especiais nomeados como proto-oncogenes. A leucemia é um dos cânceres mais conhecidos e acometem os glóbulos brancos que perdem suas funções e passam a se multiplicar descontroladamente. No entanto a terapia gênica, ainda experimental, busca substituir o gene defeituoso, pelo gene normal. O uso terapêutico de inibidores da tirosino quinase (mesilato de imatinibe), reduz significativamente a progressão da doença e elimina os principais sintomas da fase crônica da leucemia, aumentando assim a expectativa de vida. Os objetivos deste trabalho são analisar a expressão diferencial que estão relacionadas ao desenvolvimento da leucemia mediante a sua evolução ou tratamento usando Imatinibe. Os estudos foram realizados em linhagens celulares submetidos por análises de microarranjos de amostras de Leucemia Mielóide Aguda Bialélica e Leucemia Mielóide Crônica.  Foram utilizadas microarranjos da Affymetrix Gene Chip HU133 de células de Leucemia Mieloide extraídos do banco de dados Gene Expression Omnibus. Dentre os resultados obtidos o gene FCAR atua no sistema imune de pacientes com Leucemia, ele é um receptor da glicoproteína transmembrana presente, genes dessa família são usados em imunoterapia usando células T, diferencialmente expresso e está atuando na ativação do sistema imunológico no tratamento da Leucemia Mielóide Aguda. Com isso é possível utilizar as ferramentas de análise de expressão gênica como meio para localizar genes diferencialmente expressos e assim determinar novas terapias gênicas.

The Reaction of Brain Structures with OsO4-Zinc-Iodide Stain

1971 ◽  
Vol 29 ◽  
pp. 272-273
Author(s):  
Werner J. Niklowitz
Keyword(s):  
Electron Microscope ◽  
Structural Changes ◽  
Mossy Fiber ◽  
Toxic Metal ◽  
Staining Technique ◽  
Brain Structures ◽  
Oxidase Inhibitor ◽  
Fiber Layer ◽  
Hippocampal Tissue ◽  
Zinc Iodide

After intoxication of rabbits with certain substances such as convulsant agents (3-acetylpyridine), centrally acting drugs (reserpine), or toxic metal compounds (tetraethyl lead) a significant observation by phase microscope is the loss of contrast of the hippocampal mossy fiber layer. It has been suggested that this alteration, as well as changes seen with the electron microscope in the hippocampal mossy fiber boutons, may be related to a loss of neurotransmitters. The purpose of these experiments was to apply the OsO4-zinc-iodide staining technique to the study of these structural changes since it has been suggested that OsO4-zinc-iodide stain reacts with neurotransmitters (acetylcholine, catecholamines).Domestic New Zealand rabbits (2.5 to 3 kg) were used. Hippocampal tissue was removed from normal and experimental animals treated with 3-acetylpyridine (antimetabolite of nicotinamide), reserpine (anti- hypertensive/tranquilizer), or iproniazid (antidepressant/monamine oxidase inhibitor). After fixation in glutaraldehyde hippocampal tissue was treated with OsO4-zinc-iodide stain and further processed for phase and electron microscope studies.

Local mechanisms for acupoint sensitization in gall bladder meridian of foot shaoyang-a gene chip study

2019 ◽  
Vol 44 (2) ◽  
pp. 77-87
Author(s):  
Koichi Ishida ◽  
Liyue Qin ◽  
Ting Wang ◽  
Ying Lei ◽  
Weiwei Hu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gall Bladder ◽  
Interleukin 1 ◽  
Gene Chip ◽  
Molecular Evidence ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Bladder Meridian ◽  
Integrin Linked Kinase ◽  
Necrosis Factor

Acupuncture manipulations are clinically important to traditional Chinese medicine, yet the biological mechanisms have not been fully understood. This study aimed to investigate continuous stimulation-induced gene expression changes at stimulated and non-stimulated adjacent acupoints in the same meridian. Catgut embedding into acupoint (CEP) was conducted at acupoint Yanglingquan (gall bladder meridian of foot-shaoyang 34, GB34) of Sprague Dawley rats once or continuously for eight weeks, and gene expression changes at GB34 were assessed by gene chip array analysis 72 h after the last CEP treatment. A total of 688 genes exhibited opposite changes in expression between the two treatments, and 1,336 genes were regulated only by the eight-week CEP treatment. Ingenuity Pathway Analysis revealed that among these differentially regulated genes by one-time and eight-week CEP treatment, insulin-like growth factor-1 pathway and integrin-linked kinase pathway, and Wnt/~ catenin signaling pathway match the observed gene changes to predicted up/down regulation patterns. Upstream analysis further predicted six molecules, namely, tumor necrosis factor, interleukin 1~, interleukin la, kallikrein-related peptidase 5, protein kinase Ca, and catenin ~1. On the other hand, continuous eight-week CEP stimulation at acupoint Xuanzhong (GB39) caused similar changes in the expression of 32 genes at acupoints GB34 and Fengshi (GB31) on the same meridian. Taken together, our results provide the first molecular evidence for the local acupoints' mechanisms for acupoint sensitization theory, and implicate the existence of signaling pathways, either direct or indirect, between acupoints within the meridian GB.

scholarly journals Distributive characteristics of the CYP2C9 and AGTR1 genetic polymorphisms in Han Chinese hypertensive patients: a retrospective study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Keping Chen ◽  
Peng Xiao ◽  
Guochun Li ◽  
Chunling Wang ◽  
Chuankun Yang
Keyword(s):  
Genetic Polymorphisms ◽  
Venous Blood ◽  
Gene Chip ◽  
Han Chinese ◽  
Genotype Frequency ◽  
Wild Type ◽  
Angiotensin Ii Receptor ◽  
Hypertensive Patients ◽  
Genotype Frequencies ◽  
Combined Genotypes

Abstract Background There is an individual variation in response to antihypertensive effect of the angiotensin II receptor antagonist. This study aimed to determine the allele and genotype frequencies of CYP2C9 and AGTR1 genetic polymorphisms and explore the potential role of these polymorphisms in guiding the selection of angiotensinIIreceptor antagonist in Han Chinese hypertensive patients. Methods Totally 2419 Han Chinese hypertensive patients and 126 normotensive controls were recruited in this study. Venous blood samples were collected from each patient, and the genetic polymorphisms of CYP2C9 and AGTR1 were assessed using a gene chip platform. The allele and genotype frequency of each gene and the combined genotypes in this study were analyzed respectively. Results The gene chip analysis identified an allelic frequency of 96.51% for CYP2C9*1 and 3.49% for CYP2C9*3 in the cohort of Han Chinese hypertensive patients. Statistical analysis showed that the frequency of wild-type homozygous for CYP2C9*1/*1 was 93.30%, while the frequency of heterozygous for *1/*3 or mutant homozygous for *3/*3 was 6.41% or 0.29%. Meanwhile, we detected allelic frequencies of 95.06% and 4.94% for the A and C allele of AGTR1, respectively. While the genotype frequency of wild-type homozygous for AA was 90.41%, the frequency of heterozygous for AC or mutant homozygous for CC was 9.30% or 0.29%. Notably, we observed that 84.66% (2048/2419) of the subjects exhibited a combined genotype of CYP2C9 and AGTR1 as *1/*1 + AA, while the combined genotypes *3/*3 + AC or *3/*3 + CC were not detected in hypertension patients. Besides, no significant association was found between normotensive controls and hypertensive patients, or among the three grades of hypertensive patients. Conclusions These data revealed the polymorphisms characteristics of CYP2C9 and AGTR1 in Han Chinese hypertensive patients, providing valuable information for genotype-based antihypertension therapy in prospective clinical studies in the future.

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