Histamine H2-like receptors in chick cerebral cortex: effects on cyclic AMP synthesis and characterization by [3H]tiotidine binding

SummaryThe radioactive adenosine 3′,5′-monophosphate (cyclic AMP) level derived from 8-14C adenine in intact rabbit platelets decreased in the presence of mitochondrial inhibitor (potassium cyanide) or uncoupler (sodium azide), and markedly increased by the addition of NaF, monoiodoacetic acid (MIA), or 2-deoxy-D-glucose. The stimulative effect of the glycolytic inhibitors was distinctly enhanced by the simultaneous addition of sodium succinate. MIA did neither directly stimulate the adenyl cyclase activity nor inhibit the phosphodiesterase activity. These results suggest that cyclic AMP synthesis in platelets is closely linked to mitochondrial oxidative phosphorylation.

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Expression of a Gαs/Gαi Chimera That Constitutively Activates Cyclic AMP Synthesis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)83603-1 ◽

1989 ◽

Vol 264 (10) ◽

pp. 5687-5693

Author(s):

C W Woon ◽

S Soparkar ◽

L Heasley ◽

G L Johnson

Keyword(s):

Cyclic Amp ◽

Cyclic Amp Synthesis

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Interactions between adenylate cyclase and the yeast GTPase-activating protein IRA1.

Molecular and Cellular Biology ◽

10.1128/mcb.11.9.4591 ◽

1991 ◽

Vol 11 (9) ◽

pp. 4591-4598 ◽

Cited By ~ 30

Author(s):

M R Mitts ◽

J Bradshaw-Rouse ◽

W Heideman

Keyword(s):

Cyclic Amp ◽

Adenylate Cyclase ◽

Adenylate Cyclase System ◽

Gtpase Activating Protein ◽

Yeast Saccharomyces Cerevisiae ◽

Strong Candidate ◽

Sepharose 4B ◽

Stimulation Of ◽

Cyclic Amp Synthesis

The adenylate cyclase system of the yeast Saccharomyces cerevisiae contains many proteins, including the CYR1 polypeptide, which is responsible for catalyzing the formation of cyclic AMP from ATP, RAS1 and RAS2 polypeptides, which mediate stimulation of cyclic AMP synthesis by guanine nucleotides, and the yeast GTPase-activating protein analog IRA1. We have previously reported that adenylate cyclase is only peripherally bound to the yeast membrane. We have concluded that IRA1 is a strong candidate for a protein involved in anchoring adenylate cyclase to the membrane. We base this conclusion on the following criteria: (i) a disruption of the IRA1 gene produced a mutant with very low membrane-associated levels of adenylate cyclase activity, (ii) membranes made from these mutants were incapable of binding adenylate cyclase in vitro, (iii) IRA1 antibodies inhibit binding of adenylate cyclase to the membrane, and (iv) IRA1 and adenylate cyclase comigrate on Sepharose 4B.

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Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

Biochemical Journal ◽

10.1042/bj1320475 ◽

1973 ◽

Vol 132 (3) ◽

pp. 475-482 ◽

Cited By ~ 30

Author(s):

Martin Reddington ◽

Richard Rodnight ◽

Michael Williams

Keyword(s):

Cerebral Cortex ◽

Cyclic Amp ◽

Guinea Pig ◽

Electrical Pulses ◽

Phosphorus Turnover ◽

Serine Phosphate ◽

Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

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Effects of Ca ions on action potentials in immature cultured neurons from chick cerebral cortex

Journal of Cellular Physiology ◽

10.1002/jcp.1041100303 ◽

1982 ◽

Vol 110 (3) ◽

pp. 241-244 ◽

Cited By ~ 5

Author(s):

Junko Mori ◽

Hiroshi Ashida ◽

Eiichi Maru ◽

Jiro Tatsuno

Keyword(s):

Cerebral Cortex ◽

Action Potentials ◽

Cultured Neurons ◽

Ca Ions ◽

Chick Cerebral Cortex

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Involvement of adenosine-sensitive cyclic AMP-generating systems in epileptic activity of rat cerebral cortex

Neuroscience Research Supplements ◽

10.1016/0921-8696(92)90923-o ◽

1992 ◽

Vol 17 ◽

pp. 144

Author(s):

Yukio Hattori ◽

Akiyoshi Moriwaki ◽

Yasushi Hayashi ◽

Yasuo Hori

Keyword(s):

Cerebral Cortex ◽

Cyclic Amp ◽

Epileptic Activity ◽

Rat Cerebral Cortex

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Mutant analysis suggests that cyclic GMP mediates the cyclic AMP-induced Ca2+ uptake in Dictyostelium

Journal of Cell Science ◽

10.1242/jcs.99.1.187 ◽

1991 ◽

Vol 99 (1) ◽

pp. 187-191

Author(s):

S. Menz ◽

J. Bumann ◽

E. Jaworski ◽

D. Malchow

Keyword(s):

Cyclic Amp ◽

Mutant Strain ◽

Cyclic Gmp ◽

Parental Strain ◽

Dependent Manner ◽

Heavy Chains ◽

Signal Relay ◽

Sensitive Electrode ◽

Dose Dependent ◽

Cyclic Amp Synthesis

Previous work has shown that streamer F (stmF) mutants of Dictyostelium discoideum exhibit prolonged chemotactic elongation in aggregation fields. The mutants carry an altered structural gene for cyclic GMP phosphodiesterase resulting in low activities of this enzyme. Chemotactic stimulation by cyclic AMP causes a rapid transient increase in the cyclic GMP concentration followed by association of myosin heavy chains with the cytoskeleton. Both events persist several times longer in stmF mutants than in the parental strain, indicating that the change in association of myosin with the cytoskeleton is transmitted directly or indirectly by cyclic GMP. We measured the cyclic AMP-induced Ca2+ uptake with a Ca(2+)-sensitive electrode and found that Ca2+ uptake was prolonged in stmF mutants but not in the parental strain. The G alpha 2 mutant strain HC33 (fgdA), devoid of InsP3 release and receptor/guanylate cyclase coupling, lacked Ca2+ uptake. However, the latter response and cyclic GMP formation were normal in the signal-relay mutant strain agip 53 where cyclic AMP-stimulated cyclic AMP synthesis is absent. LiCl, which inhibits InsP3 formation in Dictyostelium, blocked Ca2+ uptake in a dose-dependent manner. The data indicate that the receptor-mediated Ca2+ uptake depends on the InsP3 pathway and is regulated by cyclic GMP. The rate of Ca2+ uptake was correlated in time with the association of myosin with the cytoskeleton, suggesting that Ca2+ uptake is involved in the motility response of the cells.

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Spike-shaped oscillations in the absence of measurable changes in cyclic AMP concentration in a mutant of Dictyostelium discoideum

Journal of Cell Science ◽

10.1242/jcs.87.5.723 ◽

1987 ◽

Vol 87 (5) ◽

pp. 723-730

Author(s):

B. Wurster ◽

R. Mohn

Keyword(s):

Light Scattering ◽

Cyclic Amp ◽

Cell Surface ◽

Dictyostelium Discoideum ◽

Cyclic Gmp ◽

Parent Strain ◽

Reaction System ◽

Cell Suspensions ◽

Deficient Mutant ◽

Cyclic Amp Synthesis

Periodic activities of Dictyostelium discoideum cells involve two types of oscillations, spike-shaped and sinusoidal. Spike-shaped oscillations are accompanied by the periodic synthesis and release of cyclic AMP, and cyclic AMP-activated cyclic AMP synthesis is believed to control these oscillations. Experiments described here call into question the importance of cyclic AMP in spike-shaped oscillations. Cell suspensions of strain agip43, an aggregation-deficient mutant of D. discoideum, displayed spike-shaped oscillations in light scattering with period lengths about 1.5 times larger than those of the parent strain. These oscillations were not accompanied by measurable oscillations of cyclic AMP and cyclic GMP. Applied cyclic AMP pulses elicited increases of two- to threefold in the cyclic AMP level and increases of seven- to ninefold in the cyclic GMP concentration. Cyclic AMP additions caused phase shifts in the oscillations of agip43 cells, suggesting that cyclic AMP receptors at the cell surface communicate with the oscillator. We interpret these results in terms of an oscillator not based on cyclic AMP. This oscillator should be coupled to the reaction system involving cyclic AMP synthesis and release. The latter can operate in an oscillatory manner in the parent strain Ax2 but not in mutant agip43.

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